Experiment: D-Salicin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27410 and BWI76_RS27415 overlap by 6 nucleotides BWI76_RS27415 and BWI76_RS27420 are separated by 83 nucleotides BWI76_RS27420 and BWI76_RS27425 are separated by 54 nucleotides BWI76_RS27425 and BWI76_RS27430 are separated by 18 nucleotides
BWI76_RS27410: BWI76_RS27410 - DNA ligase B, at 5,687,041 to 5,688,717
_RS27410
BWI76_RS27415: BWI76_RS27415 - hypothetical protein, at 5,688,712 to 5,688,891
_RS27415
BWI76_RS27420: BWI76_RS27420 - guanylate kinase, at 5,688,975 to 5,689,598
_RS27420
BWI76_RS27425: BWI76_RS27425 - DNA-directed RNA polymerase subunit omega, at 5,689,653 to 5,689,928
_RS27425
BWI76_RS27430: BWI76_RS27430 - (p)ppGpp synthetase, at 5,689,947 to 5,692,067
_RS27430
Position (kb) 5689
5690 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5688.693 kb on - strand at 5688.900 kb on + strand at 5688.900 kb on + strand at 5688.908 kb on - strand at 5688.926 kb on - strand at 5688.926 kb on - strand at 5688.926 kb on - strand at 5689.709 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425
Per-strain Table
Position Strand Gene LocusTag Fraction D-Salicin (C) remove 5,688,693 - +2.0 5,688,900 + -0.5 5,688,900 + -0.7 5,688,908 - -0.2 5,688,926 - -1.5 5,688,926 - +0.1 5,688,926 - +1.2 5,689,709 + BWI76_RS27425 0.20 -1.2 5,689,821 + BWI76_RS27425 0.61 -0.6 5,689,821 + BWI76_RS27425 0.61 -1.8
Or see this region's nucleotide sequence