Experiment: Ceftazidime 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24720 and AO356_24725 are separated by 195 nucleotides AO356_24725 and AO356_24730 are separated by 155 nucleotides AO356_24730 and AO356_24735 are separated by 205 nucleotides AO356_24735 and AO356_24740 overlap by 4 nucleotides
AO356_24720: AO356_24720 - DNA-binding protein, at 5,593,410 to 5,594,843
_24720
AO356_24725: AO356_24725 - alkylhydroperoxidase, at 5,595,039 to 5,595,479
_24725
AO356_24730: AO356_24730 - hypothetical protein, at 5,595,635 to 5,595,961
_24730
AO356_24735: AO356_24735 - TonB-dependent outer membrane receptor, at 5,596,167 to 5,596,799
_24735
AO356_24740: AO356_24740 - RNA polymerase subunit sigma-24, at 5,596,796 to 5,597,341
_24740
Position (kb) 5595
5596 Strain fitness (log2 ratio) -1
0
1 at 5594.660 kb on - strand, within AO356_24720 at 5594.674 kb on + strand, within AO356_24720 at 5594.675 kb on - strand, within AO356_24720 at 5594.675 kb on - strand, within AO356_24720 at 5594.791 kb on + strand at 5594.917 kb on + strand at 5594.918 kb on - strand at 5594.918 kb on - strand at 5595.060 kb on + strand at 5595.060 kb on + strand at 5595.061 kb on - strand at 5595.061 kb on - strand at 5595.061 kb on - strand at 5595.246 kb on + strand, within AO356_24725 at 5595.360 kb on + strand, within AO356_24725 at 5595.360 kb on + strand, within AO356_24725 at 5595.361 kb on - strand, within AO356_24725 at 5595.688 kb on + strand, within AO356_24730 at 5595.689 kb on - strand, within AO356_24730 at 5595.841 kb on - strand, within AO356_24730 at 5595.856 kb on - strand, within AO356_24730 at 5596.014 kb on + strand at 5596.015 kb on - strand at 5596.036 kb on - strand at 5596.401 kb on + strand, within AO356_24735 at 5596.402 kb on - strand, within AO356_24735 at 5596.494 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.532 kb on - strand, within AO356_24735 at 5596.844 kb on + strand at 5596.845 kb on - strand at 5596.920 kb on - strand, within AO356_24740
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 0.2 mg/ml remove 5,594,660 - AO356_24720 0.87 +0.1 5,594,674 + AO356_24720 0.88 -0.1 5,594,675 - AO356_24720 0.88 +0.1 5,594,675 - AO356_24720 0.88 +0.6 5,594,791 + +0.1 5,594,917 + -0.5 5,594,918 - +0.5 5,594,918 - +0.8 5,595,060 + -0.3 5,595,060 + +1.4 5,595,061 - +1.0 5,595,061 - +0.5 5,595,061 - -0.6 5,595,246 + AO356_24725 0.47 +0.5 5,595,360 + AO356_24725 0.73 +0.9 5,595,360 + AO356_24725 0.73 -0.1 5,595,361 - AO356_24725 0.73 +0.0 5,595,688 + AO356_24730 0.16 +1.0 5,595,689 - AO356_24730 0.17 +1.2 5,595,841 - AO356_24730 0.63 -0.8 5,595,856 - AO356_24730 0.68 -0.1 5,596,014 + -0.5 5,596,015 - +0.1 5,596,036 - +0.4 5,596,401 + AO356_24735 0.37 +0.6 5,596,402 - AO356_24735 0.37 +0.6 5,596,494 + AO356_24735 0.52 -0.6 5,596,531 + AO356_24735 0.58 -0.8 5,596,531 + AO356_24735 0.58 -0.4 5,596,531 + AO356_24735 0.58 -0.4 5,596,532 - AO356_24735 0.58 -0.6 5,596,844 + -0.1 5,596,845 - +0.4 5,596,920 - AO356_24740 0.23 +0.9
Or see this region's nucleotide sequence