Experiment: L-Lysine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02567 and SMc02568 overlap by 4 nucleotides SMc02568 and SMc02569 are separated by 64 nucleotides SMc02569 and SMc02570 overlap by 4 nucleotides SMc02570 and SMc02572 are separated by 32 nucleotides
SMc02567: SMc02567 - pantothenate kinase, at 56,529 to 57,524
SMc02567
SMc02568: SMc02568 - phosphoribosyl-ATP pyrophosphatase, at 57,521 to 57,844
SMc02568
SMc02569: SMc02569 - imidazole glycerol phosphate synthase subunit HisF, at 57,909 to 58,685
SMc02569
SMc02570: SMc02570 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 58,682 to 59,425
SMc02570
SMc02572: SMc02572 - imidazole glycerol phosphate synthase subunit HisH, at 59,458 to 60,108
SMc02572
Position (kb)
57
58
59 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 57.632 kb on - strand, within SMc02568 at 57.639 kb on + strand, within SMc02568 at 57.864 kb on + strand at 57.865 kb on - strand at 57.873 kb on + strand at 57.874 kb on - strand at 57.903 kb on - strand at 57.971 kb on - strand at 58.248 kb on + strand, within SMc02569 at 58.248 kb on + strand, within SMc02569 at 58.248 kb on + strand, within SMc02569 at 58.248 kb on + strand, within SMc02569 at 58.567 kb on + strand, within SMc02569 at 58.665 kb on + strand at 58.685 kb on + strand at 58.685 kb on + strand at 58.686 kb on - strand at 58.686 kb on - strand at 58.932 kb on + strand, within SMc02570 at 58.932 kb on + strand, within SMc02570 at 58.932 kb on + strand, within SMc02570 at 58.932 kb on + strand, within SMc02570 at 58.933 kb on - strand, within SMc02570 at 58.933 kb on - strand, within SMc02570 at 59.343 kb on + strand, within SMc02570 at 59.343 kb on + strand, within SMc02570 at 59.344 kb on - strand, within SMc02570 at 59.344 kb on - strand, within SMc02570 at 59.627 kb on + strand, within SMc02572 at 59.627 kb on + strand, within SMc02572 at 59.628 kb on - strand, within SMc02572
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine remove 57,632 - SMc02568 0.34 -2.7 57,639 + SMc02568 0.36 -2.5 57,864 + -4.5 57,865 - +0.6 57,873 + -3.0 57,874 - +0.0 57,903 - +0.1 57,971 - -2.7 58,248 + SMc02569 0.44 -3.5 58,248 + SMc02569 0.44 -4.4 58,248 + SMc02569 0.44 -4.4 58,248 + SMc02569 0.44 -2.9 58,567 + SMc02569 0.85 -4.0 58,665 + -5.3 58,685 + -3.7 58,685 + -0.8 58,686 - -1.0 58,686 - -0.3 58,932 + SMc02570 0.34 -5.8 58,932 + SMc02570 0.34 -3.2 58,932 + SMc02570 0.34 -3.5 58,932 + SMc02570 0.34 -0.8 58,933 - SMc02570 0.34 -0.2 58,933 - SMc02570 0.34 -5.7 59,343 + SMc02570 0.89 -5.9 59,343 + SMc02570 0.89 -2.5 59,344 - SMc02570 0.89 -2.0 59,344 - SMc02570 0.89 -2.8 59,627 + SMc02572 0.26 -4.0 59,627 + SMc02572 0.26 -4.7 59,628 - SMc02572 0.26 -6.2
Or see this region's nucleotide sequence