Experiment: L-Lysine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1525 and SMa1526 are separated by 3 nucleotides SMa1526 and SMa1529 are separated by 18 nucleotides SMa1529 and SMa1531 are separated by 16 nucleotides SMa1531 and SMa1532 overlap by 4 nucleotides
SMa1525: SMa1525 - NuoF2 NADH I chain F, at 841,280 to 842,545
SMa1525
SMa1526: SMa1526 - NuoE2 NADH I chain E, at 842,549 to 843,055
SMa1526
SMa1529: SMa1529 - NuoD2 NADH I chain D, at 843,074 to 844,288
SMa1529
SMa1531: SMa1531 - NuoC2 NADH I chain C, at 844,305 to 844,853
SMa1531
SMa1532: SMa1532 - NADH dehydrogenase subunit B, at 844,850 to 845,353
SMa1532
Position (kb) 843
844
845 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 842.153 kb on + strand, within SMa1525 at 842.166 kb on + strand, within SMa1525 at 842.167 kb on - strand, within SMa1525 at 842.167 kb on - strand, within SMa1525 at 842.182 kb on - strand, within SMa1525 at 842.182 kb on - strand, within SMa1525 at 842.187 kb on + strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.211 kb on + strand, within SMa1525 at 842.211 kb on + strand, within SMa1525 at 842.316 kb on + strand, within SMa1525 at 842.317 kb on - strand, within SMa1525 at 842.317 kb on - strand, within SMa1525 at 842.434 kb on + strand at 842.434 kb on + strand at 842.550 kb on + strand at 842.550 kb on + strand at 842.675 kb on - strand, within SMa1526 at 842.874 kb on + strand, within SMa1526 at 842.892 kb on + strand, within SMa1526 at 843.075 kb on + strand at 843.138 kb on + strand at 843.139 kb on - strand at 843.238 kb on - strand, within SMa1529 at 843.238 kb on - strand, within SMa1529 at 843.238 kb on - strand, within SMa1529 at 843.238 kb on - strand, within SMa1529 at 843.277 kb on - strand, within SMa1529 at 843.391 kb on + strand, within SMa1529 at 843.538 kb on - strand, within SMa1529 at 843.538 kb on - strand, within SMa1529 at 843.655 kb on + strand, within SMa1529 at 843.684 kb on - strand, within SMa1529 at 843.696 kb on + strand, within SMa1529 at 843.719 kb on + strand, within SMa1529 at 843.793 kb on - strand, within SMa1529 at 843.865 kb on - strand, within SMa1529 at 843.895 kb on + strand, within SMa1529 at 843.961 kb on + strand, within SMa1529 at 843.963 kb on + strand, within SMa1529 at 843.964 kb on - strand, within SMa1529 at 843.987 kb on - strand, within SMa1529 at 844.047 kb on + strand, within SMa1529 at 844.125 kb on + strand, within SMa1529 at 844.133 kb on - strand, within SMa1529 at 844.156 kb on - strand, within SMa1529 at 844.156 kb on - strand, within SMa1529 at 844.323 kb on + strand at 844.324 kb on - strand at 844.325 kb on + strand at 844.326 kb on - strand at 844.378 kb on - strand, within SMa1531 at 844.397 kb on - strand, within SMa1531 at 844.453 kb on + strand, within SMa1531 at 844.453 kb on + strand, within SMa1531 at 844.453 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.496 kb on - strand, within SMa1531 at 844.496 kb on - strand, within SMa1531 at 844.500 kb on - strand, within SMa1531 at 844.500 kb on - strand, within SMa1531 at 844.618 kb on + strand, within SMa1531 at 844.618 kb on + strand, within SMa1531 at 844.618 kb on + strand, within SMa1531 at 844.627 kb on + strand, within SMa1531 at 844.627 kb on + strand, within SMa1531 at 844.628 kb on - strand, within SMa1531 at 844.674 kb on + strand, within SMa1531 at 844.674 kb on + strand, within SMa1531 at 844.675 kb on - strand, within SMa1531 at 844.675 kb on - strand, within SMa1531 at 844.765 kb on + strand, within SMa1531 at 844.765 kb on + strand, within SMa1531 at 844.765 kb on + strand, within SMa1531 at 844.766 kb on - strand, within SMa1531 at 844.766 kb on - strand, within SMa1531 at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.861 kb on + strand at 845.016 kb on + strand, within SMa1532 at 845.016 kb on + strand, within SMa1532 at 845.016 kb on + strand, within SMa1532 at 845.017 kb on - strand, within SMa1532 at 845.017 kb on - strand, within SMa1532 at 845.017 kb on - strand, within SMa1532 at 845.025 kb on + strand, within SMa1532 at 845.025 kb on + strand, within SMa1532 at 845.026 kb on - strand, within SMa1532 at 845.202 kb on + strand, within SMa1532
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine remove 842,153 + SMa1525 0.69 -0.3 842,166 + SMa1525 0.70 +0.4 842,167 - SMa1525 0.70 -0.1 842,167 - SMa1525 0.70 -0.2 842,182 - SMa1525 0.71 +1.0 842,182 - SMa1525 0.71 -1.8 842,187 + SMa1525 0.72 -0.2 842,188 - SMa1525 0.72 -0.1 842,188 - SMa1525 0.72 -2.5 842,188 - SMa1525 0.72 -0.7 842,188 - SMa1525 0.72 +1.1 842,211 + SMa1525 0.74 -0.8 842,211 + SMa1525 0.74 +1.3 842,316 + SMa1525 0.82 -0.0 842,317 - SMa1525 0.82 +1.7 842,317 - SMa1525 0.82 +0.3 842,434 + +1.6 842,434 + -0.8 842,550 + -0.1 842,550 + +0.9 842,675 - SMa1526 0.25 +0.3 842,874 + SMa1526 0.64 -0.6 842,892 + SMa1526 0.68 -0.2 843,075 + +2.0 843,138 + -0.8 843,139 - +0.5 843,238 - SMa1529 0.13 +0.5 843,238 - SMa1529 0.13 +0.2 843,238 - SMa1529 0.13 +0.2 843,238 - SMa1529 0.13 -0.1 843,277 - SMa1529 0.17 +0.4 843,391 + SMa1529 0.26 +0.1 843,538 - SMa1529 0.38 +0.6 843,538 - SMa1529 0.38 +0.0 843,655 + SMa1529 0.48 -0.3 843,684 - SMa1529 0.50 -0.1 843,696 + SMa1529 0.51 -0.1 843,719 + SMa1529 0.53 +0.2 843,793 - SMa1529 0.59 +0.1 843,865 - SMa1529 0.65 +1.1 843,895 + SMa1529 0.68 +0.7 843,961 + SMa1529 0.73 +0.7 843,963 + SMa1529 0.73 +0.3 843,964 - SMa1529 0.73 +0.6 843,987 - SMa1529 0.75 -0.2 844,047 + SMa1529 0.80 -0.4 844,125 + SMa1529 0.87 +0.1 844,133 - SMa1529 0.87 -2.1 844,156 - SMa1529 0.89 +0.7 844,156 - SMa1529 0.89 +0.5 844,323 + +0.6 844,324 - +0.2 844,325 + +0.1 844,326 - +0.1 844,378 - SMa1531 0.13 -0.2 844,397 - SMa1531 0.17 +0.0 844,453 + SMa1531 0.27 -0.0 844,453 + SMa1531 0.27 -0.8 844,453 + SMa1531 0.27 +1.3 844,495 + SMa1531 0.35 -0.3 844,495 + SMa1531 0.35 +0.2 844,495 + SMa1531 0.35 -0.3 844,495 + SMa1531 0.35 +0.2 844,496 - SMa1531 0.35 -0.0 844,496 - SMa1531 0.35 -0.0 844,500 - SMa1531 0.36 -0.3 844,500 - SMa1531 0.36 -0.9 844,618 + SMa1531 0.57 -0.3 844,618 + SMa1531 0.57 +0.7 844,618 + SMa1531 0.57 +0.1 844,627 + SMa1531 0.59 +0.1 844,627 + SMa1531 0.59 +0.3 844,628 - SMa1531 0.59 -0.1 844,674 + SMa1531 0.67 +1.1 844,674 + SMa1531 0.67 -0.0 844,675 - SMa1531 0.67 +1.2 844,675 - SMa1531 0.67 +1.7 844,765 + SMa1531 0.84 +1.3 844,765 + SMa1531 0.84 +0.4 844,765 + SMa1531 0.84 +0.3 844,766 - SMa1531 0.84 -0.3 844,766 - SMa1531 0.84 -0.9 844,859 + -0.5 844,859 + +0.4 844,859 + -0.9 844,859 + +2.3 844,859 + +0.5 844,859 + -1.6 844,860 - -0.1 844,860 - +0.9 844,860 - -1.0 844,860 - +0.1 844,860 - +0.8 844,860 - -0.4 844,860 - +0.3 844,860 - -0.0 844,860 - -0.5 844,861 + -0.6 845,016 + SMa1532 0.33 -1.0 845,016 + SMa1532 0.33 -0.7 845,016 + SMa1532 0.33 +0.1 845,017 - SMa1532 0.33 -2.0 845,017 - SMa1532 0.33 +2.5 845,017 - SMa1532 0.33 -3.1 845,025 + SMa1532 0.35 -2.3 845,025 + SMa1532 0.35 -0.4 845,026 - SMa1532 0.35 -2.0 845,202 + SMa1532 0.70 -0.9
Or see this region's nucleotide sequence