Experiment: L-Lysine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20181 and SM_b20182 overlap by 26 nucleotides SM_b20182 and SM_b20183 overlap by 4 nucleotides SM_b20183 and SM_b20184 overlap by 4 nucleotides SM_b20184 and SM_b20185 overlap by 4 nucleotides
SM_b20181: SM_b20181 - ABC transporter substrate-binding protein, at 198,276 to 199,247
_b20181
SM_b20182: SM_b20182 - ABC transporter, at 199,222 to 200,001
_b20182
SM_b20183: SM_b20183 - ABC transporter ATP-binding protein, at 199,998 to 200,621
_b20183
SM_b20184: SM_b20184 - ABC transporter ATP-binding protein, at 200,618 to 201,346
_b20184
SM_b20185: SM_b20185 - hypothetical protein, at 201,343 to 202,134
_b20185
Position (kb) 199
200
201 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 199.245 kb on + strand at 199.272 kb on - strand at 199.409 kb on - strand, within SM_b20182 at 199.532 kb on + strand, within SM_b20182 at 199.579 kb on + strand, within SM_b20182 at 199.579 kb on + strand, within SM_b20182 at 199.580 kb on - strand, within SM_b20182 at 199.580 kb on - strand, within SM_b20182 at 199.580 kb on - strand, within SM_b20182 at 199.580 kb on - strand, within SM_b20182 at 199.806 kb on - strand, within SM_b20182 at 199.873 kb on + strand, within SM_b20182 at 199.910 kb on - strand, within SM_b20182 at 199.910 kb on - strand, within SM_b20182 at 200.421 kb on + strand, within SM_b20183 at 200.582 kb on + strand at 200.584 kb on + strand at 200.585 kb on - strand at 200.585 kb on - strand at 200.585 kb on - strand at 201.081 kb on + strand, within SM_b20184 at 201.226 kb on - strand, within SM_b20184 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.517 kb on + strand, within SM_b20185 at 201.518 kb on - strand, within SM_b20185 at 201.518 kb on - strand, within SM_b20185 at 201.519 kb on + strand, within SM_b20185 at 201.519 kb on + strand, within SM_b20185 at 201.520 kb on - strand, within SM_b20185 at 201.520 kb on - strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.522 kb on + strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.523 kb on - strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.526 kb on + strand, within SM_b20185 at 201.535 kb on + strand, within SM_b20185 at 201.535 kb on + strand, within SM_b20185 at 201.536 kb on - strand at 201.537 kb on + strand, within SM_b20185
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine remove 199,245 + -0.7 199,272 - +2.8 199,409 - SM_b20182 0.24 +0.5 199,532 + SM_b20182 0.40 -0.0 199,579 + SM_b20182 0.46 -1.7 199,579 + SM_b20182 0.46 +0.3 199,580 - SM_b20182 0.46 -0.2 199,580 - SM_b20182 0.46 +0.5 199,580 - SM_b20182 0.46 +0.3 199,580 - SM_b20182 0.46 +0.2 199,806 - SM_b20182 0.75 -0.5 199,873 + SM_b20182 0.83 +1.1 199,910 - SM_b20182 0.88 -2.5 199,910 - SM_b20182 0.88 +0.4 200,421 + SM_b20183 0.68 -0.6 200,582 + +0.8 200,584 + +0.0 200,585 - -0.3 200,585 - -0.4 200,585 - -1.2 201,081 + SM_b20184 0.64 +0.0 201,226 - SM_b20184 0.83 -0.4 201,517 + SM_b20185 0.22 -1.0 201,517 + SM_b20185 0.22 -1.4 201,517 + SM_b20185 0.22 -1.5 201,517 + SM_b20185 0.22 +0.1 201,517 + SM_b20185 0.22 +0.7 201,518 - SM_b20185 0.22 +0.0 201,518 - SM_b20185 0.22 +0.2 201,519 + SM_b20185 0.22 -0.7 201,519 + SM_b20185 0.22 -6.2 201,520 - SM_b20185 0.22 -1.0 201,520 - SM_b20185 0.22 -0.4 201,522 + SM_b20185 0.23 +0.8 201,522 + SM_b20185 0.23 +1.3 201,522 + SM_b20185 0.23 -0.4 201,522 + SM_b20185 0.23 -0.7 201,523 - SM_b20185 0.23 -0.5 201,523 - SM_b20185 0.23 +0.2 201,523 - SM_b20185 0.23 -0.5 201,523 - SM_b20185 0.23 +0.2 201,523 - SM_b20185 0.23 -0.8 201,526 + SM_b20185 0.23 -0.3 201,526 + SM_b20185 0.23 +0.1 201,526 + SM_b20185 0.23 -0.4 201,526 + SM_b20185 0.23 +0.4 201,535 + SM_b20185 0.24 -0.3 201,535 + SM_b20185 0.24 -1.5 201,536 - -1.8 201,537 + SM_b20185 0.24 -0.4
Or see this region's nucleotide sequence