Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3284 and Dshi_3285 overlap by 4 nucleotides Dshi_3285 and Dshi_3286 are separated by 15 nucleotides Dshi_3286 and Dshi_3287 are separated by 58 nucleotides
Dshi_3284: Dshi_3284 - molybdopterin-guanine dinucleotide biosynthesis protein B (RefSeq), at 3,450,303 to 3,452,075
_3284
Dshi_3285: Dshi_3285 - Thioredoxin domain (RefSeq), at 3,452,072 to 3,452,488
_3285
Dshi_3286: Dshi_3286 - cytochrome c biogenesis protein transmembrane region (RefSeq), at 3,452,504 to 3,453,217
_3286
Dshi_3287: Dshi_3287 - protein of unknown function DUF6 transmembrane (RefSeq), at 3,453,276 to 3,454,142
_3287
Position (kb)
3452
3453
3454 Strain fitness (log2 ratio)
-2
-1
0
1 at 3451.632 kb on + strand, within Dshi_3284 at 3451.812 kb on - strand, within Dshi_3284 at 3451.899 kb on - strand at 3451.910 kb on + strand at 3451.973 kb on + strand at 3451.976 kb on - strand at 3452.048 kb on + strand at 3452.061 kb on - strand at 3452.232 kb on - strand, within Dshi_3285 at 3452.312 kb on - strand, within Dshi_3285 at 3452.314 kb on + strand, within Dshi_3285 at 3452.392 kb on + strand, within Dshi_3285 at 3452.400 kb on + strand, within Dshi_3285 at 3452.406 kb on - strand, within Dshi_3285 at 3452.491 kb on - strand at 3452.543 kb on + strand at 3452.585 kb on + strand, within Dshi_3286 at 3452.600 kb on - strand, within Dshi_3286 at 3452.654 kb on - strand, within Dshi_3286 at 3452.691 kb on + strand, within Dshi_3286 at 3452.740 kb on + strand, within Dshi_3286 at 3452.798 kb on + strand, within Dshi_3286 at 3452.817 kb on - strand, within Dshi_3286 at 3452.830 kb on - strand, within Dshi_3286 at 3453.005 kb on + strand, within Dshi_3286 at 3453.033 kb on - strand, within Dshi_3286 at 3453.224 kb on + strand at 3453.232 kb on - strand at 3453.291 kb on - strand at 3453.342 kb on - strand at 3453.488 kb on + strand, within Dshi_3287 at 3453.512 kb on - strand, within Dshi_3287 at 3453.533 kb on + strand, within Dshi_3287 at 3453.615 kb on + strand, within Dshi_3287 at 3453.687 kb on - strand, within Dshi_3287 at 3453.864 kb on + strand, within Dshi_3287 at 3453.872 kb on - strand, within Dshi_3287 at 3453.886 kb on + strand, within Dshi_3287 at 3453.896 kb on + strand, within Dshi_3287 at 3454.090 kb on + strand at 3454.095 kb on + strand at 3454.103 kb on - strand at 3454.108 kb on - strand at 3454.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 3,451,632 + Dshi_3284 0.75 +0.2 3,451,812 - Dshi_3284 0.85 +1.2 3,451,899 - +1.2 3,451,910 + -0.0 3,451,973 + -0.6 3,451,976 - -1.9 3,452,048 + -0.6 3,452,061 - -0.2 3,452,232 - Dshi_3285 0.38 +0.1 3,452,312 - Dshi_3285 0.58 +0.2 3,452,314 + Dshi_3285 0.58 -1.0 3,452,392 + Dshi_3285 0.77 -0.3 3,452,400 + Dshi_3285 0.79 +0.0 3,452,406 - Dshi_3285 0.80 -1.1 3,452,491 - -0.1 3,452,543 + -0.2 3,452,585 + Dshi_3286 0.11 -0.8 3,452,600 - Dshi_3286 0.13 +0.2 3,452,654 - Dshi_3286 0.21 -1.2 3,452,691 + Dshi_3286 0.26 -0.1 3,452,740 + Dshi_3286 0.33 -0.6 3,452,798 + Dshi_3286 0.41 -0.4 3,452,817 - Dshi_3286 0.44 -1.7 3,452,830 - Dshi_3286 0.46 +0.6 3,453,005 + Dshi_3286 0.70 -0.1 3,453,033 - Dshi_3286 0.74 -1.9 3,453,224 + -0.5 3,453,232 - -0.2 3,453,291 - +0.4 3,453,342 - +0.9 3,453,488 + Dshi_3287 0.24 -0.5 3,453,512 - Dshi_3287 0.27 -0.3 3,453,533 + Dshi_3287 0.30 -0.3 3,453,615 + Dshi_3287 0.39 -0.7 3,453,687 - Dshi_3287 0.47 -0.6 3,453,864 + Dshi_3287 0.68 -0.2 3,453,872 - Dshi_3287 0.69 -0.2 3,453,886 + Dshi_3287 0.70 -0.6 3,453,896 + Dshi_3287 0.72 +0.2 3,454,090 + +0.3 3,454,095 + +0.7 3,454,103 - -0.1 3,454,108 - -0.1 3,454,128 + -0.2
Or see this region's nucleotide sequence