Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2088 and Dshi_2089 are separated by 87 nucleotides Dshi_2089 and Dshi_2090 overlap by 4 nucleotides Dshi_2090 and Dshi_2091 are separated by 52 nucleotides Dshi_2091 and Dshi_2092 are separated by 89 nucleotides
Dshi_2088: Dshi_2088 - aminotransferase class I and II (RefSeq), at 2,212,787 to 2,213,905
_2088
Dshi_2089: Dshi_2089 - hypothetical protein (RefSeq), at 2,213,993 to 2,214,208
_2089
Dshi_2090: Dshi_2090 - Serine--glyoxylate transaminase (RefSeq), at 2,214,205 to 2,215,461
_2090
Dshi_2091: Dshi_2091 - glycosyl transferase family 2 (RefSeq), at 2,215,514 to 2,216,209
_2091
Dshi_2092: Dshi_2092 - glycine cleavage T protein (aminomethyl transferase) (RefSeq), at 2,216,299 to 2,217,084
_2092
Position (kb)
2214
2215
2216 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2213.240 kb on + strand, within Dshi_2088 at 2213.293 kb on + strand, within Dshi_2088 at 2213.295 kb on - strand, within Dshi_2088 at 2213.386 kb on - strand, within Dshi_2088 at 2213.476 kb on + strand, within Dshi_2088 at 2213.669 kb on + strand, within Dshi_2088 at 2213.723 kb on + strand, within Dshi_2088 at 2213.877 kb on - strand at 2214.050 kb on - strand, within Dshi_2089 at 2214.271 kb on + strand at 2214.325 kb on - strand at 2214.411 kb on - strand, within Dshi_2090 at 2214.536 kb on - strand, within Dshi_2090 at 2214.650 kb on + strand, within Dshi_2090 at 2214.704 kb on + strand, within Dshi_2090 at 2214.708 kb on + strand, within Dshi_2090 at 2214.742 kb on - strand, within Dshi_2090 at 2214.988 kb on - strand, within Dshi_2090 at 2215.066 kb on + strand, within Dshi_2090 at 2215.074 kb on - strand, within Dshi_2090 at 2215.159 kb on - strand, within Dshi_2090 at 2215.192 kb on - strand, within Dshi_2090 at 2215.228 kb on + strand, within Dshi_2090 at 2215.242 kb on + strand, within Dshi_2090 at 2215.242 kb on + strand, within Dshi_2090 at 2215.290 kb on - strand, within Dshi_2090 at 2215.310 kb on + strand, within Dshi_2090 at 2215.336 kb on - strand at 2215.478 kb on - strand at 2215.497 kb on - strand at 2215.497 kb on - strand at 2215.511 kb on + strand at 2215.637 kb on - strand, within Dshi_2091 at 2215.705 kb on - strand at 2215.778 kb on - strand, within Dshi_2091 at 2215.785 kb on + strand, within Dshi_2091 at 2215.793 kb on - strand, within Dshi_2091 at 2215.828 kb on - strand, within Dshi_2091 at 2216.135 kb on - strand, within Dshi_2091 at 2216.147 kb on - strand at 2216.205 kb on + strand at 2216.213 kb on - strand at 2216.310 kb on + strand at 2216.318 kb on - strand at 2216.318 kb on - strand at 2216.318 kb on - strand at 2216.318 kb on - strand at 2216.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 2,213,240 + Dshi_2088 0.40 +0.2 2,213,293 + Dshi_2088 0.45 +0.6 2,213,295 - Dshi_2088 0.45 -1.2 2,213,386 - Dshi_2088 0.54 -1.3 2,213,476 + Dshi_2088 0.62 -0.1 2,213,669 + Dshi_2088 0.79 -0.5 2,213,723 + Dshi_2088 0.84 -0.1 2,213,877 - -0.7 2,214,050 - Dshi_2089 0.26 -0.0 2,214,271 + -0.6 2,214,325 - -1.3 2,214,411 - Dshi_2090 0.16 -1.2 2,214,536 - Dshi_2090 0.26 -0.4 2,214,650 + Dshi_2090 0.35 -0.3 2,214,704 + Dshi_2090 0.40 -0.1 2,214,708 + Dshi_2090 0.40 -0.5 2,214,742 - Dshi_2090 0.43 -1.2 2,214,988 - Dshi_2090 0.62 -3.2 2,215,066 + Dshi_2090 0.68 -0.3 2,215,074 - Dshi_2090 0.69 -0.2 2,215,159 - Dshi_2090 0.76 -0.5 2,215,192 - Dshi_2090 0.79 +1.0 2,215,228 + Dshi_2090 0.81 -0.8 2,215,242 + Dshi_2090 0.82 -0.5 2,215,242 + Dshi_2090 0.82 -0.5 2,215,290 - Dshi_2090 0.86 +0.1 2,215,310 + Dshi_2090 0.88 -0.5 2,215,336 - -0.5 2,215,478 - -0.7 2,215,497 - -0.7 2,215,497 - +1.1 2,215,511 + +0.0 2,215,637 - Dshi_2091 0.18 +0.0 2,215,705 - +0.4 2,215,778 - Dshi_2091 0.38 +0.4 2,215,785 + Dshi_2091 0.39 -0.2 2,215,793 - Dshi_2091 0.40 +0.2 2,215,828 - Dshi_2091 0.45 +1.1 2,216,135 - Dshi_2091 0.89 -1.3 2,216,147 - -0.2 2,216,205 + -0.8 2,216,213 - +0.4 2,216,310 + -1.4 2,216,318 - -0.1 2,216,318 - +0.4 2,216,318 - +0.1 2,216,318 - -0.9 2,216,318 - +0.2
Or see this region's nucleotide sequence