Experiment: L-Lysine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0513 and SMa0514 are separated by 1 nucleotides SMa0514 and SMa0516 overlap by 4 nucleotides SMa0516 and SMa0518 overlap by 4 nucleotides SMa0518 and SMa0520 are separated by 358 nucleotides
SMa0513: SMa0513 - gluconate 5-dehydrogenase, at 277,846 to 278,601
SMa0513
SMa0514: SMa0514 - gluconate kinase, at 278,603 to 279,121
SMa0514
SMa0516: SMa0516 - hypothetical protein, at 279,118 to 280,005
SMa0516
SMa0518: SMa0518 - hypothetical protein, at 280,002 to 280,334
SMa0518
SMa0520: SMa0520 - RpiR family transcriptional regulator, at 280,693 to 281,535
SMa0520
Position (kb) 279
280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 278.308 kb on + strand, within SMa0513 at 278.309 kb on - strand, within SMa0513 at 278.309 kb on - strand, within SMa0513 at 278.569 kb on + strand at 278.570 kb on - strand at 278.570 kb on - strand at 278.705 kb on + strand, within SMa0514 at 278.705 kb on + strand, within SMa0514 at 278.706 kb on - strand, within SMa0514 at 278.706 kb on - strand, within SMa0514 at 279.298 kb on + strand, within SMa0516 at 279.299 kb on - strand, within SMa0516 at 279.299 kb on - strand, within SMa0516 at 279.299 kb on - strand, within SMa0516 at 279.305 kb on + strand, within SMa0516 at 279.306 kb on - strand, within SMa0516 at 279.592 kb on + strand, within SMa0516 at 279.592 kb on + strand, within SMa0516 at 279.600 kb on + strand, within SMa0516 at 279.647 kb on + strand, within SMa0516 at 279.648 kb on - strand, within SMa0516 at 279.648 kb on - strand, within SMa0516 at 279.648 kb on - strand, within SMa0516 at 279.698 kb on + strand, within SMa0516 at 279.775 kb on + strand, within SMa0516 at 279.776 kb on - strand, within SMa0516 at 279.776 kb on - strand, within SMa0516 at 279.776 kb on - strand, within SMa0516 at 279.987 kb on + strand at 280.168 kb on + strand, within SMa0518 at 280.191 kb on + strand, within SMa0518 at 280.191 kb on + strand, within SMa0518 at 280.191 kb on + strand, within SMa0518 at 280.192 kb on - strand, within SMa0518 at 280.373 kb on + strand at 280.487 kb on + strand at 280.487 kb on + strand at 280.487 kb on + strand at 280.487 kb on + strand at 280.487 kb on + strand at 280.487 kb on + strand at 280.488 kb on - strand at 280.488 kb on - strand at 280.488 kb on - strand at 280.488 kb on - strand at 280.533 kb on + strand at 280.560 kb on + strand at 280.667 kb on - strand at 280.667 kb on - strand at 280.669 kb on - strand at 280.719 kb on + strand at 280.719 kb on + strand at 280.729 kb on - strand at 280.879 kb on + strand, within SMa0520 at 280.880 kb on - strand, within SMa0520 at 280.880 kb on - strand, within SMa0520 at 280.950 kb on + strand, within SMa0520 at 280.951 kb on - strand, within SMa0520
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine remove 278,308 + SMa0513 0.61 -0.5 278,309 - SMa0513 0.61 -0.1 278,309 - SMa0513 0.61 +0.1 278,569 + +2.6 278,570 - +0.6 278,570 - +0.9 278,705 + SMa0514 0.20 -1.0 278,705 + SMa0514 0.20 -0.5 278,706 - SMa0514 0.20 +0.8 278,706 - SMa0514 0.20 +0.0 279,298 + SMa0516 0.20 +1.2 279,299 - SMa0516 0.20 -0.9 279,299 - SMa0516 0.20 +0.1 279,299 - SMa0516 0.20 +0.4 279,305 + SMa0516 0.21 +2.2 279,306 - SMa0516 0.21 +0.5 279,592 + SMa0516 0.53 -1.2 279,592 + SMa0516 0.53 -0.4 279,600 + SMa0516 0.54 -0.3 279,647 + SMa0516 0.60 -0.9 279,648 - SMa0516 0.60 -0.2 279,648 - SMa0516 0.60 -0.6 279,648 - SMa0516 0.60 -0.7 279,698 + SMa0516 0.65 -0.3 279,775 + SMa0516 0.74 +0.1 279,776 - SMa0516 0.74 -0.2 279,776 - SMa0516 0.74 -0.4 279,776 - SMa0516 0.74 +0.7 279,987 + +1.0 280,168 + SMa0518 0.50 +0.3 280,191 + SMa0518 0.57 +0.2 280,191 + SMa0518 0.57 +1.3 280,191 + SMa0518 0.57 -0.4 280,192 - SMa0518 0.57 +0.1 280,373 + -2.8 280,487 + +1.6 280,487 + +0.3 280,487 + +0.2 280,487 + +0.6 280,487 + +0.0 280,487 + -0.5 280,488 - -0.1 280,488 - -1.1 280,488 - -0.2 280,488 - -0.2 280,533 + +0.0 280,560 + -0.1 280,667 - -1.2 280,667 - +1.3 280,669 - -0.8 280,719 + +0.6 280,719 + -0.5 280,729 - -0.0 280,879 + SMa0520 0.22 +0.3 280,880 - SMa0520 0.22 +0.4 280,880 - SMa0520 0.22 +0.2 280,950 + SMa0520 0.30 +0.3 280,951 - SMa0520 0.31 +0.6
Or see this region's nucleotide sequence