Experiment: Lomefloxacin 0.0005 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02500 and AO356_02505 are separated by 275 nucleotides AO356_02505 and AO356_02510 are separated by 64 nucleotides AO356_02510 and AO356_02515 are separated by 142 nucleotides AO356_02515 and AO356_02520 overlap by 55 nucleotides
AO356_02500: AO356_02500 - 2-alkenal reductase, at 542,300 to 543,334
_02500
AO356_02505: AO356_02505 - nucleoid-associated protein, at 543,610 to 543,948
_02505
AO356_02510: AO356_02510 - DNA polymerase III subunit gamma/tau, at 544,013 to 546,088
_02510
AO356_02515: AO356_02515 - amino acid ABC transporter substrate-binding protein, at 546,231 to 547,001
_02515
AO356_02520: AO356_02520 - diguanylate cyclase, at 546,947 to 548,695
_02520
Position (kb)
544
545
546
547 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 543.144 kb on + strand, within AO356_02500 at 543.144 kb on + strand, within AO356_02500 at 543.145 kb on - strand, within AO356_02500 at 543.145 kb on - strand, within AO356_02500 at 543.177 kb on + strand, within AO356_02500 at 543.178 kb on - strand, within AO356_02500 at 543.366 kb on + strand at 543.366 kb on + strand at 543.366 kb on + strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.367 kb on - strand at 543.368 kb on + strand at 543.368 kb on + strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.369 kb on - strand at 543.447 kb on + strand at 543.514 kb on + strand at 543.514 kb on + strand at 543.514 kb on + strand at 543.514 kb on + strand at 543.523 kb on + strand at 543.524 kb on - strand at 543.539 kb on + strand at 543.584 kb on + strand at 543.585 kb on - strand at 543.585 kb on - strand at 543.585 kb on - strand at 544.014 kb on + strand at 544.014 kb on + strand at 544.014 kb on + strand at 544.015 kb on - strand at 546.167 kb on + strand at 546.167 kb on + strand at 546.168 kb on - strand at 546.168 kb on - strand at 546.168 kb on - strand at 546.176 kb on + strand at 546.176 kb on + strand at 546.177 kb on - strand at 546.177 kb on - strand at 546.177 kb on - strand at 546.177 kb on - strand at 546.181 kb on + strand at 546.181 kb on + strand at 546.182 kb on - strand at 546.182 kb on - strand at 546.212 kb on + strand at 546.212 kb on + strand at 546.231 kb on - strand at 546.231 kb on - strand at 546.231 kb on - strand at 546.231 kb on - strand at 546.231 kb on - strand at 546.403 kb on + strand, within AO356_02515 at 546.403 kb on + strand, within AO356_02515 at 546.403 kb on + strand, within AO356_02515 at 546.403 kb on + strand, within AO356_02515 at 546.486 kb on + strand, within AO356_02515 at 546.486 kb on + strand, within AO356_02515 at 546.486 kb on + strand, within AO356_02515 at 546.487 kb on - strand, within AO356_02515 at 546.487 kb on - strand, within AO356_02515 at 546.487 kb on - strand, within AO356_02515 at 546.487 kb on - strand, within AO356_02515 at 546.487 kb on - strand, within AO356_02515 at 546.523 kb on - strand, within AO356_02515 at 546.523 kb on - strand, within AO356_02515 at 546.758 kb on + strand, within AO356_02515 at 546.759 kb on - strand, within AO356_02515 at 546.794 kb on + strand, within AO356_02515 at 546.795 kb on - strand, within AO356_02515 at 546.852 kb on + strand, within AO356_02515 at 546.852 kb on + strand, within AO356_02515 at 546.853 kb on - strand, within AO356_02515 at 546.853 kb on - strand, within AO356_02515 at 546.901 kb on + strand, within AO356_02515 at 546.901 kb on + strand, within AO356_02515 at 546.902 kb on - strand, within AO356_02515 at 546.902 kb on - strand, within AO356_02515 at 546.948 kb on + strand at 546.949 kb on - strand at 546.949 kb on - strand at 546.949 kb on - strand at 547.059 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0005 mM remove 543,144 + AO356_02500 0.82 -0.0 543,144 + AO356_02500 0.82 -0.1 543,145 - AO356_02500 0.82 +0.4 543,145 - AO356_02500 0.82 +0.6 543,177 + AO356_02500 0.85 +1.7 543,178 - AO356_02500 0.85 -0.1 543,366 + -0.2 543,366 + +1.1 543,366 + -0.7 543,367 - -0.4 543,367 - +0.1 543,367 - +1.3 543,367 - -0.8 543,367 - -0.2 543,367 - -0.0 543,367 - -2.4 543,367 - -0.7 543,367 - -1.4 543,367 - +2.0 543,368 + -0.7 543,368 + -0.5 543,369 - -1.1 543,369 - +0.0 543,369 - +0.5 543,447 + -0.8 543,514 + -0.3 543,514 + -0.2 543,514 + -0.9 543,514 + +0.3 543,523 + +1.0 543,524 - -1.6 543,539 + +1.0 543,584 + +0.1 543,585 - -1.7 543,585 - +1.0 543,585 - -0.3 544,014 + -0.8 544,014 + -1.3 544,014 + -1.9 544,015 - -1.4 546,167 + -0.5 546,167 + -1.5 546,168 - -0.6 546,168 - +0.1 546,168 - +0.4 546,176 + -1.7 546,176 + -0.4 546,177 - -1.5 546,177 - -1.0 546,177 - +0.8 546,177 - +0.6 546,181 + -3.5 546,181 + -2.7 546,182 - -0.7 546,182 - +0.2 546,212 + +0.2 546,212 + +0.2 546,231 - -1.7 546,231 - -0.7 546,231 - -0.6 546,231 - -0.1 546,231 - +1.4 546,403 + AO356_02515 0.22 -0.3 546,403 + AO356_02515 0.22 +0.4 546,403 + AO356_02515 0.22 -1.3 546,403 + AO356_02515 0.22 +0.8 546,486 + AO356_02515 0.33 +0.2 546,486 + AO356_02515 0.33 +0.1 546,486 + AO356_02515 0.33 -3.2 546,487 - AO356_02515 0.33 -2.0 546,487 - AO356_02515 0.33 -0.5 546,487 - AO356_02515 0.33 -1.5 546,487 - AO356_02515 0.33 +0.7 546,487 - AO356_02515 0.33 -0.6 546,523 - AO356_02515 0.38 +1.3 546,523 - AO356_02515 0.38 -0.6 546,758 + AO356_02515 0.68 -1.0 546,759 - AO356_02515 0.68 -1.0 546,794 + AO356_02515 0.73 +0.2 546,795 - AO356_02515 0.73 -0.5 546,852 + AO356_02515 0.81 -2.2 546,852 + AO356_02515 0.81 +0.1 546,853 - AO356_02515 0.81 +0.2 546,853 - AO356_02515 0.81 -1.3 546,901 + AO356_02515 0.87 -0.8 546,901 + AO356_02515 0.87 -0.6 546,902 - AO356_02515 0.87 +0.0 546,902 - AO356_02515 0.87 +0.2 546,948 + +0.1 546,949 - +0.5 546,949 - +0.2 546,949 - -0.6 547,059 - -0.4
Or see this region's nucleotide sequence