Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2358 and Dshi_2359 are separated by 515 nucleotides Dshi_2359 and Dshi_2360 are separated by 125 nucleotides Dshi_2360 and Dshi_2361 overlap by 8 nucleotides
Dshi_2358: Dshi_2358 - Lysine exporter protein (LYSE/YGGA) (RefSeq), at 2,492,678 to 2,493,325
_2358
Dshi_2359: Dshi_2359 - hypothetical protein (RefSeq), at 2,493,841 to 2,495,091
_2359
Dshi_2360: Dshi_2360 - urease accessory protein UreG (RefSeq), at 2,495,217 to 2,495,825
_2360
Dshi_2361: Dshi_2361 - Carboxymuconolactone decarboxylase (RefSeq), at 2,495,818 to 2,496,201
_2361
Position (kb)
2493
2494
2495
2496 Strain fitness (log2 ratio)
-1
0
1 at 2492.908 kb on - strand, within Dshi_2358 at 2492.920 kb on - strand, within Dshi_2358 at 2492.970 kb on + strand, within Dshi_2358 at 2492.970 kb on + strand, within Dshi_2358 at 2492.997 kb on + strand, within Dshi_2358 at 2493.070 kb on + strand, within Dshi_2358 at 2493.155 kb on + strand, within Dshi_2358 at 2493.163 kb on - strand, within Dshi_2358 at 2493.188 kb on + strand, within Dshi_2358 at 2493.201 kb on - strand, within Dshi_2358 at 2493.201 kb on - strand, within Dshi_2358 at 2493.235 kb on - strand, within Dshi_2358 at 2493.240 kb on + strand, within Dshi_2358 at 2493.250 kb on + strand, within Dshi_2358 at 2493.288 kb on + strand at 2493.358 kb on + strand at 2493.390 kb on - strand at 2493.455 kb on + strand at 2493.463 kb on - strand at 2493.524 kb on + strand at 2493.570 kb on - strand at 2493.763 kb on + strand at 2493.778 kb on + strand at 2493.831 kb on + strand at 2493.919 kb on + strand at 2494.006 kb on + strand, within Dshi_2359 at 2494.043 kb on + strand, within Dshi_2359 at 2494.049 kb on - strand, within Dshi_2359 at 2494.455 kb on - strand, within Dshi_2359 at 2494.491 kb on - strand, within Dshi_2359 at 2494.565 kb on + strand, within Dshi_2359 at 2494.570 kb on + strand, within Dshi_2359 at 2494.577 kb on - strand, within Dshi_2359 at 2494.677 kb on - strand, within Dshi_2359 at 2494.900 kb on - strand, within Dshi_2359 at 2495.212 kb on + strand at 2495.334 kb on - strand, within Dshi_2360 at 2495.388 kb on - strand, within Dshi_2360 at 2495.405 kb on + strand, within Dshi_2360 at 2495.438 kb on - strand, within Dshi_2360 at 2495.438 kb on - strand, within Dshi_2360 at 2495.502 kb on + strand, within Dshi_2360 at 2495.616 kb on + strand, within Dshi_2360 at 2495.632 kb on + strand, within Dshi_2360 at 2495.700 kb on - strand, within Dshi_2360 at 2495.725 kb on - strand, within Dshi_2360 at 2495.738 kb on + strand, within Dshi_2360 at 2495.746 kb on - strand, within Dshi_2360 at 2495.751 kb on - strand, within Dshi_2360 at 2495.946 kb on - strand, within Dshi_2361 at 2495.997 kb on - strand, within Dshi_2361 at 2496.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,492,908 - Dshi_2358 0.35 +1.3 2,492,920 - Dshi_2358 0.37 +1.3 2,492,970 + Dshi_2358 0.45 +0.6 2,492,970 + Dshi_2358 0.45 -1.5 2,492,997 + Dshi_2358 0.49 -0.1 2,493,070 + Dshi_2358 0.60 +0.4 2,493,155 + Dshi_2358 0.74 -0.2 2,493,163 - Dshi_2358 0.75 -0.1 2,493,188 + Dshi_2358 0.79 -0.3 2,493,201 - Dshi_2358 0.81 +0.0 2,493,201 - Dshi_2358 0.81 +0.2 2,493,235 - Dshi_2358 0.86 +0.6 2,493,240 + Dshi_2358 0.87 +0.3 2,493,250 + Dshi_2358 0.88 +0.4 2,493,288 + +1.1 2,493,358 + -0.7 2,493,390 - +0.2 2,493,455 + -1.3 2,493,463 - +0.1 2,493,524 + +0.0 2,493,570 - +0.2 2,493,763 + -0.5 2,493,778 + -1.5 2,493,831 + +0.4 2,493,919 + -0.0 2,494,006 + Dshi_2359 0.13 -0.4 2,494,043 + Dshi_2359 0.16 -1.4 2,494,049 - Dshi_2359 0.17 -0.1 2,494,455 - Dshi_2359 0.49 -0.8 2,494,491 - Dshi_2359 0.52 +0.4 2,494,565 + Dshi_2359 0.58 -0.0 2,494,570 + Dshi_2359 0.58 -0.7 2,494,577 - Dshi_2359 0.59 -0.6 2,494,677 - Dshi_2359 0.67 -1.0 2,494,900 - Dshi_2359 0.85 -0.4 2,495,212 + -0.9 2,495,334 - Dshi_2360 0.19 -0.8 2,495,388 - Dshi_2360 0.28 +0.0 2,495,405 + Dshi_2360 0.31 -0.9 2,495,438 - Dshi_2360 0.36 +0.6 2,495,438 - Dshi_2360 0.36 +0.0 2,495,502 + Dshi_2360 0.47 -0.4 2,495,616 + Dshi_2360 0.66 -0.1 2,495,632 + Dshi_2360 0.68 -0.8 2,495,700 - Dshi_2360 0.79 -0.3 2,495,725 - Dshi_2360 0.83 +0.6 2,495,738 + Dshi_2360 0.86 -0.3 2,495,746 - Dshi_2360 0.87 +0.7 2,495,751 - Dshi_2360 0.88 -0.7 2,495,946 - Dshi_2361 0.33 +0.6 2,495,997 - Dshi_2361 0.47 +0.5 2,496,069 - -0.7
Or see this region's nucleotide sequence