Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc04004 and SMc04005 are separated by 237 nucleotides SMc04005 and SMc04006 overlap by 1 nucleotides SMc04006 and SMc04007 are separated by 229 nucleotides
SMc04004: SMc04004 - signal peptide protein, at 2,999,157 to 2,999,771
SMc04004
SMc04005: SMc04005 - pyruvate kinase, at 3,000,009 to 3,001,448
SMc04005
SMc04006: SMc04006 - hypothetical protein, at 3,001,448 to 3,001,885
SMc04006
SMc04007: SMc04007 - hypothetical protein, at 3,002,115 to 3,002,909
SMc04007
Position (kb)
3000
3001
3002 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2999.039 kb on + strand at 2999.505 kb on + strand, within SMc04004 at 2999.506 kb on - strand, within SMc04004 at 2999.578 kb on + strand, within SMc04004 at 2999.578 kb on + strand, within SMc04004 at 2999.579 kb on - strand, within SMc04004 at 2999.665 kb on + strand, within SMc04004 at 2999.700 kb on + strand, within SMc04004 at 2999.700 kb on + strand, within SMc04004 at 2999.701 kb on - strand, within SMc04004 at 2999.701 kb on - strand, within SMc04004 at 2999.701 kb on - strand, within SMc04004 at 2999.896 kb on + strand at 2999.897 kb on - strand at 3000.040 kb on + strand at 3000.041 kb on - strand at 3000.164 kb on + strand, within SMc04005 at 3000.165 kb on - strand, within SMc04005 at 3000.165 kb on - strand, within SMc04005 at 3000.319 kb on + strand, within SMc04005 at 3000.319 kb on + strand, within SMc04005 at 3000.320 kb on - strand, within SMc04005 at 3000.419 kb on - strand, within SMc04005 at 3000.496 kb on + strand, within SMc04005 at 3000.557 kb on + strand, within SMc04005 at 3000.959 kb on + strand, within SMc04005 at 3000.959 kb on + strand, within SMc04005 at 3001.126 kb on + strand, within SMc04005 at 3002.038 kb on + strand at 3002.039 kb on - strand at 3002.092 kb on - strand at 3002.124 kb on + strand at 3002.125 kb on - strand at 3002.133 kb on + strand at 3002.134 kb on - strand at 3002.211 kb on + strand, within SMc04007 at 3002.211 kb on + strand, within SMc04007 at 3002.260 kb on - strand, within SMc04007 at 3002.264 kb on + strand, within SMc04007
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 2,999,039 + +1.0 2,999,505 + SMc04004 0.57 +0.8 2,999,506 - SMc04004 0.57 +1.2 2,999,578 + SMc04004 0.68 +0.7 2,999,578 + SMc04004 0.68 -0.2 2,999,579 - SMc04004 0.69 +1.4 2,999,665 + SMc04004 0.83 +0.4 2,999,700 + SMc04004 0.88 +0.2 2,999,700 + SMc04004 0.88 +0.0 2,999,701 - SMc04004 0.88 -0.4 2,999,701 - SMc04004 0.88 -1.5 2,999,701 - SMc04004 0.88 -0.7 2,999,896 + +0.4 2,999,897 - +0.2 3,000,040 + -0.4 3,000,041 - -2.0 3,000,164 + SMc04005 0.11 -1.5 3,000,165 - SMc04005 0.11 -0.0 3,000,165 - SMc04005 0.11 -0.9 3,000,319 + SMc04005 0.22 +0.8 3,000,319 + SMc04005 0.22 -1.2 3,000,320 - SMc04005 0.22 -1.3 3,000,419 - SMc04005 0.28 +1.4 3,000,496 + SMc04005 0.34 -0.2 3,000,557 + SMc04005 0.38 -0.9 3,000,959 + SMc04005 0.66 -1.4 3,000,959 + SMc04005 0.66 -1.0 3,001,126 + SMc04005 0.78 -1.4 3,002,038 + -0.2 3,002,039 - +0.1 3,002,092 - +0.8 3,002,124 + +0.0 3,002,125 - +2.8 3,002,133 + +0.4 3,002,134 - -0.5 3,002,211 + SMc04007 0.12 +0.4 3,002,211 + SMc04007 0.12 -0.3 3,002,260 - SMc04007 0.18 +0.3 3,002,264 + SMc04007 0.19 +2.4
Or see this region's nucleotide sequence