Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02245 and SMc02246 overlap by 4 nucleotides SMc02246 and SMc02247 are separated by 120 nucleotides SMc02247 and SMc02248 are separated by 141 nucleotides
SMc02245: SMc02245 - dihydroorotate dehydrogenase 2, at 608,536 to 609,624
SMc02245
SMc02246: SMc02246 - hypothetical protein, at 609,621 to 609,968
SMc02246
SMc02247: SMc02247 - signal peptide protein, at 610,089 to 610,526
SMc02247
SMc02248: SMc02248 - transcriptional regulator, at 610,668 to 611,315
SMc02248
Position (kb) 609
610 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 608.678 kb on + strand, within SMc02245 at 608.679 kb on - strand, within SMc02245 at 609.167 kb on + strand, within SMc02245 at 609.167 kb on + strand, within SMc02245 at 609.208 kb on - strand, within SMc02245 at 609.280 kb on - strand, within SMc02245 at 609.930 kb on + strand, within SMc02246 at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.953 kb on - strand at 609.953 kb on - strand at 609.953 kb on - strand at 609.954 kb on + strand at 609.955 kb on - strand at 609.959 kb on + strand at 610.176 kb on + strand, within SMc02247 at 610.596 kb on + strand at 610.596 kb on + strand at 610.597 kb on - strand at 610.597 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 608,678 + SMc02245 0.13 -4.8 608,679 - SMc02245 0.13 -2.8 609,167 + SMc02245 0.58 -3.0 609,167 + SMc02245 0.58 -5.0 609,208 - SMc02245 0.62 -1.8 609,280 - SMc02245 0.68 -4.7 609,930 + SMc02246 0.89 -2.7 609,952 + -2.0 609,952 + -6.4 609,952 + -4.3 609,952 + -2.4 609,952 + -4.6 609,953 - +0.5 609,953 - -0.5 609,953 - -3.5 609,954 + -3.7 609,955 - -2.3 609,959 + -2.4 610,176 + SMc02247 0.20 -1.3 610,596 + +0.5 610,596 + -0.1 610,597 - +0.3 610,597 - -0.4
Or see this region's nucleotide sequence