Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00851 and SMc00850 are separated by 59 nucleotides SMc00850 and SMc00849 are separated by 129 nucleotides SMc00849 and SMc00993 are separated by 116 nucleotides
SMc00851: SMc00851 - glycyl-tRNA synthetase subunit beta, at 935,222 to 937,387
SMc00851
SMc00850: SMc00850 - hypothetical protein, at 937,447 to 937,965
SMc00850
SMc00849: SMc00849 - hypothetical protein, at 938,095 to 938,577
SMc00849
SMc00993: SMc00993 - phosphoribosylamine--glycine ligase, at 938,694 to 939,965
SMc00993
Position (kb)
937
938 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 937.808 kb on - strand, within SMc00850 at 937.808 kb on - strand, within SMc00850 at 937.888 kb on + strand, within SMc00850 at 937.904 kb on + strand, within SMc00850 at 937.904 kb on + strand, within SMc00850 at 937.935 kb on + strand at 938.082 kb on + strand at 938.088 kb on + strand at 938.088 kb on + strand at 938.089 kb on - strand at 938.089 kb on - strand at 938.089 kb on - strand at 938.155 kb on - strand, within SMc00849 at 938.208 kb on + strand, within SMc00849 at 938.208 kb on + strand, within SMc00849 at 938.208 kb on + strand, within SMc00849 at 938.208 kb on + strand, within SMc00849 at 938.209 kb on - strand, within SMc00849 at 938.417 kb on + strand, within SMc00849 at 938.543 kb on + strand at 938.543 kb on + strand at 938.608 kb on + strand at 938.615 kb on - strand at 938.646 kb on - strand at 938.741 kb on - strand at 938.779 kb on + strand at 938.780 kb on - strand at 938.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 937,808 - SMc00850 0.70 +0.3 937,808 - SMc00850 0.70 +0.2 937,888 + SMc00850 0.85 -0.2 937,904 + SMc00850 0.88 +0.5 937,904 + SMc00850 0.88 -0.7 937,935 + +0.6 938,082 + +0.1 938,088 + -0.5 938,088 + -0.8 938,089 - +0.5 938,089 - -0.0 938,089 - +1.2 938,155 - SMc00849 0.12 +0.6 938,208 + SMc00849 0.23 -0.4 938,208 + SMc00849 0.23 +0.1 938,208 + SMc00849 0.23 +1.2 938,208 + SMc00849 0.23 +2.3 938,209 - SMc00849 0.24 +0.0 938,417 + SMc00849 0.67 -0.6 938,543 + +0.5 938,543 + -0.6 938,608 + +1.7 938,615 - -0.5 938,646 - +0.1 938,741 - -1.3 938,779 + -5.9 938,780 - -4.8 938,780 - -5.4
Or see this region's nucleotide sequence