Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00814 and SMc00815 are separated by 190 nucleotides SMc00815 and SMc00816 are separated by 60 nucleotides SMc00816 and SMc00817 are separated by 114 nucleotides
SMc00814: SMc00814 - signal peptide protein, at 835,041 to 836,021
SMc00814
SMc00815: SMc00815 - inosine 5'-monophosphate dehydrogenase, at 836,212 to 837,714
SMc00815
SMc00816: SMc00816 - hypothetical protein, at 837,775 to 838,191
SMc00816
SMc00817: SMc00817 - carboxymethylenebutenolidase (dienelactone hydrolase) protein, at 838,306 to 839,181
SMc00817
Position (kb)
836
837
838 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 835.452 kb on + strand, within SMc00814 at 835.452 kb on + strand, within SMc00814 at 835.453 kb on - strand, within SMc00814 at 835.506 kb on - strand, within SMc00814 at 835.506 kb on - strand, within SMc00814 at 835.554 kb on + strand, within SMc00814 at 835.555 kb on - strand, within SMc00814 at 835.555 kb on - strand, within SMc00814 at 835.576 kb on - strand, within SMc00814 at 835.745 kb on + strand, within SMc00814 at 835.745 kb on + strand, within SMc00814 at 835.745 kb on + strand, within SMc00814 at 835.745 kb on + strand, within SMc00814 at 835.745 kb on + strand, within SMc00814 at 835.745 kb on + strand, within SMc00814 at 835.746 kb on - strand, within SMc00814 at 835.753 kb on - strand, within SMc00814 at 835.809 kb on + strand, within SMc00814 at 835.810 kb on - strand, within SMc00814 at 835.810 kb on - strand, within SMc00814 at 835.810 kb on - strand, within SMc00814 at 835.810 kb on - strand, within SMc00814 at 835.984 kb on - strand at 836.004 kb on + strand at 836.005 kb on - strand at 836.110 kb on + strand at 836.955 kb on + strand, within SMc00815 at 837.448 kb on + strand, within SMc00815 at 837.515 kb on - strand, within SMc00815 at 837.578 kb on - strand at 837.605 kb on - strand at 837.715 kb on + strand at 837.715 kb on + strand at 838.208 kb on - strand at 838.232 kb on + strand at 838.233 kb on - strand at 838.266 kb on - strand at 838.266 kb on - strand at 838.266 kb on - strand at 838.345 kb on + strand at 838.354 kb on + strand at 838.354 kb on + strand at 838.355 kb on - strand at 838.356 kb on + strand at 838.356 kb on + strand at 838.357 kb on - strand at 838.497 kb on + strand, within SMc00817 at 838.498 kb on - strand, within SMc00817 at 838.631 kb on + strand, within SMc00817 at 838.632 kb on - strand, within SMc00817 at 838.632 kb on - strand, within SMc00817 at 838.632 kb on - strand, within SMc00817 at 838.632 kb on - strand, within SMc00817 at 838.632 kb on - strand, within SMc00817
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 835,452 + SMc00814 0.42 -0.2 835,452 + SMc00814 0.42 -0.1 835,453 - SMc00814 0.42 +0.2 835,506 - SMc00814 0.47 -0.1 835,506 - SMc00814 0.47 +0.5 835,554 + SMc00814 0.52 +0.0 835,555 - SMc00814 0.52 +0.6 835,555 - SMc00814 0.52 +0.4 835,576 - SMc00814 0.55 -0.5 835,745 + SMc00814 0.72 +0.2 835,745 + SMc00814 0.72 -1.0 835,745 + SMc00814 0.72 +0.7 835,745 + SMc00814 0.72 +0.8 835,745 + SMc00814 0.72 +0.4 835,745 + SMc00814 0.72 -1.9 835,746 - SMc00814 0.72 +0.5 835,753 - SMc00814 0.73 +1.0 835,809 + SMc00814 0.78 +0.5 835,810 - SMc00814 0.78 -0.2 835,810 - SMc00814 0.78 +0.5 835,810 - SMc00814 0.78 -0.0 835,810 - SMc00814 0.78 -1.1 835,984 - +0.5 836,004 + +0.8 836,005 - +0.1 836,110 + -0.1 836,955 + SMc00815 0.49 -1.8 837,448 + SMc00815 0.82 -3.3 837,515 - SMc00815 0.87 -3.3 837,578 - -3.3 837,605 - -4.3 837,715 + +0.4 837,715 + +0.9 838,208 - -0.5 838,232 + +1.7 838,233 - -1.0 838,266 - -1.6 838,266 - -0.5 838,266 - -0.3 838,345 + -0.2 838,354 + -0.0 838,354 + +0.2 838,355 - -0.6 838,356 + +0.2 838,356 + -0.0 838,357 - -1.0 838,497 + SMc00817 0.22 +0.7 838,498 - SMc00817 0.22 +0.1 838,631 + SMc00817 0.37 -0.7 838,632 - SMc00817 0.37 -0.2 838,632 - SMc00817 0.37 -2.1 838,632 - SMc00817 0.37 +0.2 838,632 - SMc00817 0.37 -0.3 838,632 - SMc00817 0.37 -0.8
Or see this region's nucleotide sequence