Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20503 and SM_b20504 are separated by 65 nucleotides SM_b20504 and SM_b20505 are separated by 270 nucleotides
SM_b20503: SM_b20503 - sugar ABC transporter ATP-binding protein, at 524,197 to 525,735
_b20503
SM_b20504: SM_b20504 - sugar ABC transporter substrate-binding protein, at 525,801 to 526,802
_b20504
SM_b20505: SM_b20505 - trifolitoxin immunity protein, at 527,073 to 527,861
_b20505
Position (kb)
525
526
527 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 524.965 kb on + strand, within SM_b20503 at 525.077 kb on + strand, within SM_b20503 at 525.078 kb on - strand, within SM_b20503 at 525.078 kb on - strand, within SM_b20503 at 525.078 kb on - strand, within SM_b20503 at 525.142 kb on - strand, within SM_b20503 at 525.142 kb on - strand, within SM_b20503 at 525.144 kb on + strand, within SM_b20503 at 525.144 kb on + strand, within SM_b20503 at 525.145 kb on - strand, within SM_b20503 at 525.146 kb on + strand, within SM_b20503 at 525.146 kb on + strand, within SM_b20503 at 525.146 kb on + strand, within SM_b20503 at 525.146 kb on + strand, within SM_b20503 at 525.146 kb on + strand, within SM_b20503 at 525.146 kb on + strand, within SM_b20503 at 525.146 kb on + strand, within SM_b20503 at 525.147 kb on - strand, within SM_b20503 at 525.147 kb on - strand, within SM_b20503 at 525.147 kb on - strand, within SM_b20503 at 525.147 kb on - strand, within SM_b20503 at 525.147 kb on - strand, within SM_b20503 at 525.386 kb on + strand, within SM_b20503 at 525.387 kb on - strand, within SM_b20503 at 525.661 kb on + strand at 525.661 kb on + strand at 525.803 kb on - strand at 525.822 kb on - strand at 525.838 kb on + strand at 525.999 kb on - strand, within SM_b20504 at 525.999 kb on - strand, within SM_b20504 at 526.000 kb on + strand, within SM_b20504 at 526.000 kb on + strand, within SM_b20504 at 526.001 kb on - strand, within SM_b20504 at 526.065 kb on + strand, within SM_b20504 at 526.068 kb on - strand, within SM_b20504 at 526.068 kb on - strand, within SM_b20504 at 526.068 kb on - strand, within SM_b20504 at 526.213 kb on + strand, within SM_b20504 at 526.213 kb on + strand, within SM_b20504 at 526.213 kb on + strand, within SM_b20504 at 526.214 kb on - strand, within SM_b20504 at 526.214 kb on - strand, within SM_b20504 at 526.349 kb on - strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.393 kb on + strand, within SM_b20504 at 526.394 kb on - strand, within SM_b20504 at 526.394 kb on - strand, within SM_b20504 at 526.394 kb on - strand, within SM_b20504 at 526.394 kb on - strand, within SM_b20504 at 526.394 kb on - strand, within SM_b20504 at 526.397 kb on + strand, within SM_b20504 at 526.397 kb on + strand, within SM_b20504 at 526.426 kb on + strand, within SM_b20504 at 526.427 kb on - strand, within SM_b20504 at 526.432 kb on - strand, within SM_b20504 at 526.603 kb on + strand, within SM_b20504 at 526.622 kb on - strand, within SM_b20504 at 526.622 kb on - strand, within SM_b20504 at 526.685 kb on - strand, within SM_b20504 at 526.718 kb on - strand at 526.928 kb on + strand at 526.938 kb on + strand at 526.939 kb on - strand at 527.017 kb on - strand at 527.017 kb on - strand at 527.042 kb on + strand at 527.043 kb on - strand at 527.061 kb on + strand at 527.062 kb on - strand at 527.062 kb on - strand at 527.188 kb on + strand, within SM_b20505 at 527.188 kb on + strand, within SM_b20505 at 527.274 kb on + strand, within SM_b20505 at 527.274 kb on + strand, within SM_b20505 at 527.274 kb on + strand, within SM_b20505 at 527.274 kb on + strand, within SM_b20505 at 527.275 kb on - strand, within SM_b20505 at 527.275 kb on - strand, within SM_b20505 at 527.275 kb on - strand, within SM_b20505 at 527.333 kb on - strand, within SM_b20505 at 527.353 kb on + strand, within SM_b20505 at 527.353 kb on + strand, within SM_b20505 at 527.644 kb on + strand, within SM_b20505 at 527.658 kb on + strand, within SM_b20505 at 527.741 kb on - strand, within SM_b20505 at 527.741 kb on - strand, within SM_b20505 at 527.741 kb on - strand, within SM_b20505 at 527.741 kb on - strand, within SM_b20505 at 527.741 kb on - strand, within SM_b20505 at 527.759 kb on + strand, within SM_b20505 at 527.759 kb on + strand, within SM_b20505 at 527.759 kb on + strand, within SM_b20505
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 524,965 + SM_b20503 0.50 -0.3 525,077 + SM_b20503 0.57 +0.4 525,078 - SM_b20503 0.57 -0.1 525,078 - SM_b20503 0.57 +0.6 525,078 - SM_b20503 0.57 -0.1 525,142 - SM_b20503 0.61 +0.9 525,142 - SM_b20503 0.61 -0.3 525,144 + SM_b20503 0.62 +2.5 525,144 + SM_b20503 0.62 +0.1 525,145 - SM_b20503 0.62 +1.1 525,146 + SM_b20503 0.62 +0.9 525,146 + SM_b20503 0.62 -0.4 525,146 + SM_b20503 0.62 -0.3 525,146 + SM_b20503 0.62 +0.1 525,146 + SM_b20503 0.62 +0.4 525,146 + SM_b20503 0.62 +0.2 525,146 + SM_b20503 0.62 +0.3 525,147 - SM_b20503 0.62 +0.2 525,147 - SM_b20503 0.62 -1.8 525,147 - SM_b20503 0.62 -0.7 525,147 - SM_b20503 0.62 +1.5 525,147 - SM_b20503 0.62 -0.4 525,386 + SM_b20503 0.77 -0.8 525,387 - SM_b20503 0.77 +0.4 525,661 + +0.1 525,661 + +1.6 525,803 - +0.2 525,822 - -0.8 525,838 + +1.9 525,999 - SM_b20504 0.20 +0.8 525,999 - SM_b20504 0.20 -1.7 526,000 + SM_b20504 0.20 +0.2 526,000 + SM_b20504 0.20 -1.1 526,001 - SM_b20504 0.20 -0.2 526,065 + SM_b20504 0.26 -1.4 526,068 - SM_b20504 0.27 +0.0 526,068 - SM_b20504 0.27 -0.2 526,068 - SM_b20504 0.27 +0.9 526,213 + SM_b20504 0.41 +0.1 526,213 + SM_b20504 0.41 -0.4 526,213 + SM_b20504 0.41 -0.0 526,214 - SM_b20504 0.41 -0.8 526,214 - SM_b20504 0.41 +2.9 526,349 - SM_b20504 0.55 -2.7 526,393 + SM_b20504 0.59 -1.5 526,393 + SM_b20504 0.59 -0.7 526,393 + SM_b20504 0.59 +0.2 526,393 + SM_b20504 0.59 -0.2 526,393 + SM_b20504 0.59 -0.1 526,393 + SM_b20504 0.59 -0.6 526,393 + SM_b20504 0.59 +1.4 526,393 + SM_b20504 0.59 -1.0 526,394 - SM_b20504 0.59 +0.0 526,394 - SM_b20504 0.59 -1.1 526,394 - SM_b20504 0.59 -0.4 526,394 - SM_b20504 0.59 -0.1 526,394 - SM_b20504 0.59 +0.1 526,397 + SM_b20504 0.59 -1.5 526,397 + SM_b20504 0.59 -2.7 526,426 + SM_b20504 0.62 -0.3 526,427 - SM_b20504 0.62 -0.1 526,432 - SM_b20504 0.63 -0.2 526,603 + SM_b20504 0.80 -0.3 526,622 - SM_b20504 0.82 +0.9 526,622 - SM_b20504 0.82 -0.5 526,685 - SM_b20504 0.88 +1.9 526,718 - +0.0 526,928 + +0.1 526,938 + -0.8 526,939 - +0.8 527,017 - +0.2 527,017 - -2.3 527,042 + -0.0 527,043 - +0.1 527,061 + +0.2 527,062 - +0.2 527,062 - +0.4 527,188 + SM_b20505 0.15 -0.4 527,188 + SM_b20505 0.15 +0.5 527,274 + SM_b20505 0.25 +1.3 527,274 + SM_b20505 0.25 +0.5 527,274 + SM_b20505 0.25 -0.1 527,274 + SM_b20505 0.25 +0.4 527,275 - SM_b20505 0.26 +0.3 527,275 - SM_b20505 0.26 -0.5 527,275 - SM_b20505 0.26 -0.2 527,333 - SM_b20505 0.33 -0.8 527,353 + SM_b20505 0.35 -0.3 527,353 + SM_b20505 0.35 +0.6 527,644 + SM_b20505 0.72 +0.2 527,658 + SM_b20505 0.74 +1.2 527,741 - SM_b20505 0.85 -0.7 527,741 - SM_b20505 0.85 +0.0 527,741 - SM_b20505 0.85 -0.2 527,741 - SM_b20505 0.85 +1.5 527,741 - SM_b20505 0.85 +0.3 527,759 + SM_b20505 0.87 -0.1 527,759 + SM_b20505 0.87 +0.5 527,759 + SM_b20505 0.87 +0.3
Or see this region's nucleotide sequence