Experiment: rubidium chloride 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_628 and Pf6N2E2_629 are separated by 92 nucleotides Pf6N2E2_629 and Pf6N2E2_630 overlap by 8 nucleotides Pf6N2E2_630 and Pf6N2E2_631 are separated by 63 nucleotides
Pf6N2E2_628 - 2-ketogluconate transporter, at 715,226 to 716,524
_628
Pf6N2E2_629 - 2-ketogluconate kinase (EC 2.7.1.13), at 716,617 to 717,591
_629
Pf6N2E2_630 - Epimerase KguE, at 717,584 to 718,366
_630
Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455
_631
Position (kb) 716
717
718 Strain fitness (log2 ratio) -2
-1
0
1
2 at 715.695 kb on + strand, within Pf6N2E2_628 at 715.854 kb on + strand, within Pf6N2E2_628 at 716.149 kb on - strand, within Pf6N2E2_628 at 716.149 kb on - strand, within Pf6N2E2_628 at 716.149 kb on - strand, within Pf6N2E2_628 at 716.310 kb on + strand, within Pf6N2E2_628 at 716.430 kb on + strand at 716.430 kb on + strand at 716.460 kb on + strand at 716.460 kb on + strand at 716.460 kb on + strand at 716.487 kb on + strand at 716.487 kb on + strand at 716.487 kb on + strand at 716.487 kb on + strand at 716.488 kb on - strand at 716.888 kb on + strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.698 kb on + strand, within Pf6N2E2_630 at 717.699 kb on - strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 718.071 kb on - strand, within Pf6N2E2_630 at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.371 kb on - strand at 718.371 kb on - strand at 718.373 kb on + strand at 718.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction rubidium chloride 0.25 M remove 715,695 + Pf6N2E2_628 0.36 +0.3 715,854 + Pf6N2E2_628 0.48 +0.4 716,149 - Pf6N2E2_628 0.71 -1.0 716,149 - Pf6N2E2_628 0.71 +1.1 716,149 - Pf6N2E2_628 0.71 -0.4 716,310 + Pf6N2E2_628 0.83 -0.3 716,430 + +0.2 716,430 + -2.2 716,460 + -0.5 716,460 + +0.5 716,460 + +0.2 716,487 + -1.2 716,487 + -0.6 716,487 + +0.6 716,487 + -0.4 716,488 - +2.4 716,888 + Pf6N2E2_629 0.28 -0.5 716,889 - Pf6N2E2_629 0.28 -1.7 716,889 - Pf6N2E2_629 0.28 +0.7 717,272 + Pf6N2E2_629 0.67 -1.9 717,272 + Pf6N2E2_629 0.67 -0.1 717,272 + Pf6N2E2_629 0.67 -0.5 717,698 + Pf6N2E2_630 0.15 -0.2 717,699 - Pf6N2E2_630 0.15 -0.4 717,813 + Pf6N2E2_630 0.29 -0.1 717,813 + Pf6N2E2_630 0.29 -0.9 717,813 + Pf6N2E2_630 0.29 -1.0 717,814 - Pf6N2E2_630 0.29 +1.4 717,814 - Pf6N2E2_630 0.29 +0.0 718,071 - Pf6N2E2_630 0.62 +0.7 718,370 + -0.1 718,370 + +0.4 718,370 + +1.4 718,370 + +1.0 718,370 + -1.6 718,371 - -0.3 718,371 - -0.0 718,373 + -1.3 718,373 + +0.3
Or see this region's nucleotide sequence