Experiment: rubidium chloride 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6046 and Pf6N2E2_6047 are separated by 330 nucleotides Pf6N2E2_6047 and Pf6N2E2_6048 are separated by 56 nucleotides Pf6N2E2_6048 and Pf6N2E2_6049 are separated by 355 nucleotides
Pf6N2E2_6046 - hypothetical protein, at 6,866,368 to 6,866,778
_6046
Pf6N2E2_6047 - hypothetical protein, at 6,867,109 to 6,867,405
_6047
Pf6N2E2_6048 - hypothetical protein, at 6,867,462 to 6,867,776
_6048
Pf6N2E2_6049 - hydrolase, haloacid dehalogenase-like family, at 6,868,132 to 6,868,827
_6049
Position (kb)
6867
6868 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6866.119 kb on - strand at 6866.119 kb on - strand at 6866.127 kb on - strand at 6866.181 kb on + strand at 6866.181 kb on + strand at 6866.181 kb on + strand at 6866.181 kb on + strand at 6866.181 kb on + strand at 6866.182 kb on - strand at 6866.182 kb on - strand at 6866.182 kb on - strand at 6866.185 kb on + strand at 6866.185 kb on + strand at 6866.185 kb on + strand at 6866.185 kb on + strand at 6866.185 kb on + strand at 6866.185 kb on + strand at 6866.186 kb on - strand at 6866.186 kb on - strand at 6866.186 kb on - strand at 6866.186 kb on - strand at 6866.186 kb on - strand at 6866.186 kb on - strand at 6866.186 kb on - strand at 6866.186 kb on - strand at 6866.213 kb on + strand at 6866.213 kb on + strand at 6866.214 kb on - strand at 6866.254 kb on - strand at 6866.306 kb on + strand at 6866.364 kb on + strand at 6866.365 kb on - strand at 6866.365 kb on - strand at 6866.393 kb on + strand at 6866.405 kb on + strand at 6866.405 kb on + strand at 6866.406 kb on - strand at 6866.406 kb on - strand at 6866.406 kb on - strand at 6866.406 kb on - strand at 6866.406 kb on - strand at 6866.471 kb on + strand, within Pf6N2E2_6046 at 6866.471 kb on + strand, within Pf6N2E2_6046 at 6866.471 kb on + strand, within Pf6N2E2_6046 at 6866.472 kb on - strand, within Pf6N2E2_6046 at 6866.472 kb on - strand, within Pf6N2E2_6046 at 6866.472 kb on - strand, within Pf6N2E2_6046 at 6866.753 kb on - strand at 6866.753 kb on - strand at 6867.002 kb on - strand at 6867.002 kb on - strand at 6867.003 kb on + strand at 6867.003 kb on + strand at 6867.003 kb on + strand at 6867.003 kb on + strand at 6867.003 kb on + strand at 6867.003 kb on + strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.004 kb on - strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.084 kb on + strand at 6867.085 kb on - strand at 6867.085 kb on - strand at 6867.085 kb on - strand at 6867.085 kb on - strand at 6867.085 kb on - strand at 6867.085 kb on - strand at 6867.085 kb on - strand at 6867.105 kb on + strand at 6867.105 kb on + strand at 6867.105 kb on + strand at 6867.105 kb on + strand at 6867.105 kb on + strand at 6867.106 kb on - strand at 6867.106 kb on - strand at 6867.106 kb on - strand at 6867.106 kb on - strand at 6867.247 kb on + strand, within Pf6N2E2_6047 at 6867.324 kb on - strand, within Pf6N2E2_6047 at 6867.467 kb on + strand at 6867.874 kb on + strand at 6867.955 kb on - strand at 6868.216 kb on + strand, within Pf6N2E2_6049 at 6868.216 kb on + strand, within Pf6N2E2_6049 at 6868.216 kb on + strand, within Pf6N2E2_6049 at 6868.217 kb on - strand, within Pf6N2E2_6049 at 6868.217 kb on - strand, within Pf6N2E2_6049 at 6868.217 kb on - strand, within Pf6N2E2_6049 at 6868.217 kb on - strand, within Pf6N2E2_6049 at 6868.217 kb on - strand, within Pf6N2E2_6049 at 6868.217 kb on - strand, within Pf6N2E2_6049 at 6868.319 kb on - strand, within Pf6N2E2_6049 at 6868.319 kb on - strand, within Pf6N2E2_6049
Per-strain Table
Position Strand Gene LocusTag Fraction rubidium chloride 0.25 M remove 6,866,119 - -0.7 6,866,119 - -0.1 6,866,127 - +0.9 6,866,181 + +0.8 6,866,181 + +0.2 6,866,181 + +0.1 6,866,181 + +0.6 6,866,181 + -1.6 6,866,182 - +1.0 6,866,182 - -2.2 6,866,182 - +0.3 6,866,185 + +0.3 6,866,185 + -0.3 6,866,185 + +0.2 6,866,185 + -0.4 6,866,185 + +0.7 6,866,185 + +0.4 6,866,186 - -0.0 6,866,186 - -0.1 6,866,186 - +0.5 6,866,186 - +0.3 6,866,186 - +1.2 6,866,186 - +0.4 6,866,186 - -1.7 6,866,186 - -0.4 6,866,213 + -1.5 6,866,213 + +1.0 6,866,214 - -0.2 6,866,254 - +1.2 6,866,306 + +0.7 6,866,364 + -0.6 6,866,365 - -1.2 6,866,365 - +0.0 6,866,393 + -0.1 6,866,405 + -1.8 6,866,405 + +0.9 6,866,406 - -1.6 6,866,406 - -0.0 6,866,406 - -0.8 6,866,406 - +0.3 6,866,406 - +0.4 6,866,471 + Pf6N2E2_6046 0.25 +0.4 6,866,471 + Pf6N2E2_6046 0.25 +0.9 6,866,471 + Pf6N2E2_6046 0.25 +2.3 6,866,472 - Pf6N2E2_6046 0.25 +0.5 6,866,472 - Pf6N2E2_6046 0.25 +0.7 6,866,472 - Pf6N2E2_6046 0.25 +0.5 6,866,753 - -0.2 6,866,753 - +0.0 6,867,002 - +1.1 6,867,002 - +0.6 6,867,003 + +0.6 6,867,003 + +0.5 6,867,003 + -1.4 6,867,003 + +0.7 6,867,003 + -0.1 6,867,003 + +1.3 6,867,004 - +0.3 6,867,004 - -1.5 6,867,004 - -0.1 6,867,004 - +0.4 6,867,004 - -0.4 6,867,004 - +1.6 6,867,004 - -1.5 6,867,004 - +0.3 6,867,004 - -0.4 6,867,004 - +0.4 6,867,004 - +0.3 6,867,004 - -1.7 6,867,004 - +0.7 6,867,084 + +0.1 6,867,084 + +0.1 6,867,084 + +0.1 6,867,084 + -0.3 6,867,084 + -0.3 6,867,084 + +0.1 6,867,084 + +0.9 6,867,084 + +0.1 6,867,084 + -0.1 6,867,084 + +0.6 6,867,084 + -0.5 6,867,084 + -0.3 6,867,084 + +0.9 6,867,085 - +1.1 6,867,085 - +0.2 6,867,085 - +0.1 6,867,085 - +0.0 6,867,085 - +1.1 6,867,085 - +1.4 6,867,085 - +1.4 6,867,105 + +0.8 6,867,105 + -1.2 6,867,105 + -1.5 6,867,105 + +0.9 6,867,105 + +0.6 6,867,106 - +1.3 6,867,106 - +0.1 6,867,106 - +0.8 6,867,106 - +0.4 6,867,247 + Pf6N2E2_6047 0.46 -0.4 6,867,324 - Pf6N2E2_6047 0.72 -0.6 6,867,467 + -0.3 6,867,874 + +0.7 6,867,955 - +0.7 6,868,216 + Pf6N2E2_6049 0.12 +0.9 6,868,216 + Pf6N2E2_6049 0.12 +0.6 6,868,216 + Pf6N2E2_6049 0.12 +0.0 6,868,217 - Pf6N2E2_6049 0.12 +1.5 6,868,217 - Pf6N2E2_6049 0.12 +2.2 6,868,217 - Pf6N2E2_6049 0.12 -1.8 6,868,217 - Pf6N2E2_6049 0.12 +1.2 6,868,217 - Pf6N2E2_6049 0.12 +0.6 6,868,217 - Pf6N2E2_6049 0.12 +0.7 6,868,319 - Pf6N2E2_6049 0.27 +0.2 6,868,319 - Pf6N2E2_6049 0.27 -1.0
Or see this region's nucleotide sequence