Experiment: rubidium chloride 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5041 and Pf6N2E2_5042 are separated by 128 nucleotides Pf6N2E2_5042 and Pf6N2E2_5043 are separated by 21 nucleotides Pf6N2E2_5043 and Pf6N2E2_5044 are separated by 169 nucleotides Pf6N2E2_5044 and Pf6N2E2_5045 are separated by 161 nucleotides
Pf6N2E2_5041 - Uncharacterized protein conserved in bacteria, at 5,817,920 to 5,818,669
_5041
Pf6N2E2_5042 - FIG00954572: hypothetical protein, at 5,818,798 to 5,819,277
_5042
Pf6N2E2_5043 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 5,819,299 to 5,819,910
_5043
Pf6N2E2_5044 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,820,080 to 5,820,532
_5044
Pf6N2E2_5045 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,820,694 to 5,821,596
_5045
Position (kb)
5819
5820 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5818.349 kb on + strand, within Pf6N2E2_5041 at 5818.349 kb on + strand, within Pf6N2E2_5041 at 5818.350 kb on - strand, within Pf6N2E2_5041 at 5818.586 kb on + strand, within Pf6N2E2_5041 at 5818.587 kb on - strand, within Pf6N2E2_5041 at 5818.587 kb on - strand, within Pf6N2E2_5041 at 5818.622 kb on + strand at 5818.623 kb on - strand at 5818.623 kb on - strand at 5818.711 kb on - strand at 5818.740 kb on - strand at 5818.740 kb on - strand at 5818.740 kb on - strand at 5818.837 kb on + strand at 5818.837 kb on + strand at 5818.838 kb on - strand at 5818.838 kb on - strand at 5818.840 kb on + strand at 5818.840 kb on + strand at 5818.840 kb on + strand at 5818.840 kb on + strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.859 kb on - strand, within Pf6N2E2_5042 at 5818.876 kb on + strand, within Pf6N2E2_5042 at 5818.927 kb on + strand, within Pf6N2E2_5042 at 5818.927 kb on + strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.934 kb on - strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand at 5819.188 kb on + strand, within Pf6N2E2_5042 at 5819.908 kb on + strand at 5819.908 kb on + strand at 5819.909 kb on - strand at 5819.909 kb on - strand at 5819.909 kb on - strand at 5820.081 kb on + strand at 5820.081 kb on + strand at 5820.295 kb on - strand, within Pf6N2E2_5044 at 5820.384 kb on + strand, within Pf6N2E2_5044 at 5820.385 kb on - strand, within Pf6N2E2_5044 at 5820.457 kb on + strand, within Pf6N2E2_5044 at 5820.522 kb on + strand at 5820.523 kb on - strand at 5820.526 kb on + strand at 5820.526 kb on + strand at 5820.527 kb on - strand at 5820.527 kb on - strand at 5820.535 kb on - strand at 5820.535 kb on - strand at 5820.604 kb on + strand at 5820.615 kb on + strand at 5820.655 kb on + strand at 5820.655 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.769 kb on + strand at 5820.770 kb on - strand at 5820.853 kb on + strand, within Pf6N2E2_5045 at 5820.853 kb on + strand, within Pf6N2E2_5045 at 5820.854 kb on - strand, within Pf6N2E2_5045 at 5820.854 kb on - strand, within Pf6N2E2_5045
Per-strain Table
Position Strand Gene LocusTag Fraction rubidium chloride 0.25 M remove 5,818,349 + Pf6N2E2_5041 0.57 +0.9 5,818,349 + Pf6N2E2_5041 0.57 -0.3 5,818,350 - Pf6N2E2_5041 0.57 -1.1 5,818,586 + Pf6N2E2_5041 0.89 -0.4 5,818,587 - Pf6N2E2_5041 0.89 -0.6 5,818,587 - Pf6N2E2_5041 0.89 +0.5 5,818,622 + -1.1 5,818,623 - -0.3 5,818,623 - -0.2 5,818,711 - +0.6 5,818,740 - -0.3 5,818,740 - +0.4 5,818,740 - -0.5 5,818,837 + -1.3 5,818,837 + +0.8 5,818,838 - -1.0 5,818,838 - -0.7 5,818,840 + +0.4 5,818,840 + -1.1 5,818,840 + +0.1 5,818,840 + +0.7 5,818,841 - -0.3 5,818,841 - -0.4 5,818,841 - -0.4 5,818,841 - -1.1 5,818,841 - +0.3 5,818,841 - -0.4 5,818,841 - +0.1 5,818,859 - Pf6N2E2_5042 0.13 -1.7 5,818,876 + Pf6N2E2_5042 0.16 -0.2 5,818,927 + Pf6N2E2_5042 0.27 -1.1 5,818,927 + Pf6N2E2_5042 0.27 +0.3 5,818,928 - Pf6N2E2_5042 0.27 +0.1 5,818,928 - Pf6N2E2_5042 0.27 +0.3 5,818,928 - Pf6N2E2_5042 0.27 -0.2 5,818,928 - Pf6N2E2_5042 0.27 -1.5 5,818,928 - Pf6N2E2_5042 0.27 +0.0 5,818,928 - Pf6N2E2_5042 0.27 +0.6 5,818,934 - Pf6N2E2_5042 0.28 +1.2 5,819,017 + Pf6N2E2_5042 0.46 +0.7 5,819,017 + Pf6N2E2_5042 0.46 +0.9 5,819,017 + Pf6N2E2_5042 0.46 -0.6 5,819,017 + Pf6N2E2_5042 0.46 +1.6 5,819,017 + Pf6N2E2_5042 0.46 +0.6 5,819,017 + Pf6N2E2_5042 0.46 -0.0 5,819,018 - Pf6N2E2_5042 0.46 -0.8 5,819,018 - Pf6N2E2_5042 0.46 -1.4 5,819,018 - Pf6N2E2_5042 0.46 +0.4 5,819,018 - Pf6N2E2_5042 0.46 +0.3 5,819,018 - Pf6N2E2_5042 0.46 -0.3 5,819,018 - +1.2 5,819,188 + Pf6N2E2_5042 0.81 +0.8 5,819,908 + -0.5 5,819,908 + +1.3 5,819,909 - +0.6 5,819,909 - +1.4 5,819,909 - -0.8 5,820,081 + +0.4 5,820,081 + -0.2 5,820,295 - Pf6N2E2_5044 0.47 -0.4 5,820,384 + Pf6N2E2_5044 0.67 -1.6 5,820,385 - Pf6N2E2_5044 0.67 -0.6 5,820,457 + Pf6N2E2_5044 0.83 +0.3 5,820,522 + +0.2 5,820,523 - +0.4 5,820,526 + -0.2 5,820,526 + +0.1 5,820,527 - -0.2 5,820,527 - +0.8 5,820,535 - -0.2 5,820,535 - +0.5 5,820,604 + +0.2 5,820,615 + +0.4 5,820,655 + +2.2 5,820,655 + +0.2 5,820,748 + -0.0 5,820,748 + +0.4 5,820,748 + +0.5 5,820,748 + -0.3 5,820,748 + +0.9 5,820,748 + +0.6 5,820,748 + +0.6 5,820,748 + +0.6 5,820,748 + +0.8 5,820,748 + +0.5 5,820,748 + -0.1 5,820,748 + +1.7 5,820,749 - -1.1 5,820,749 - -1.4 5,820,749 - -0.1 5,820,749 - -0.4 5,820,749 - -0.9 5,820,749 - -0.2 5,820,749 - -0.7 5,820,749 - -0.8 5,820,769 + +0.3 5,820,770 - -2.0 5,820,853 + Pf6N2E2_5045 0.18 +0.8 5,820,853 + Pf6N2E2_5045 0.18 +0.6 5,820,854 - Pf6N2E2_5045 0.18 +0.4 5,820,854 - Pf6N2E2_5045 0.18 -0.1
Or see this region's nucleotide sequence