Experiment: rubidium chloride 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4247 and Pf6N2E2_4248 overlap by 7 nucleotides Pf6N2E2_4248 and Pf6N2E2_4249 are separated by 153 nucleotides Pf6N2E2_4249 and Pf6N2E2_4250 overlap by 14 nucleotides
Pf6N2E2_4247 - O-antigen acetylase, at 4,940,633 to 4,941,787
_4247
Pf6N2E2_4248 - SAM-dependent methyltransferases, at 4,941,781 to 4,942,605
_4248
Pf6N2E2_4249 - FIG01055313: hypothetical protein, at 4,942,759 to 4,943,952
_4249
Pf6N2E2_4250 - FIG00964095: hypothetical protein, at 4,943,939 to 4,946,089
_4250
Position (kb)
4942
4943
4944 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4942.212 kb on - strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.238 kb on - strand, within Pf6N2E2_4248 at 4942.301 kb on - strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.375 kb on - strand, within Pf6N2E2_4248 at 4942.396 kb on + strand, within Pf6N2E2_4248 at 4942.406 kb on - strand, within Pf6N2E2_4248 at 4942.406 kb on - strand, within Pf6N2E2_4248 at 4942.576 kb on - strand at 4942.576 kb on - strand at 4942.576 kb on - strand at 4942.576 kb on - strand at 4942.585 kb on - strand at 4942.604 kb on - strand at 4942.727 kb on + strand at 4942.742 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.852 kb on + strand at 4942.853 kb on - strand at 4942.853 kb on - strand at 4942.853 kb on - strand at 4942.853 kb on - strand at 4943.132 kb on + strand, within Pf6N2E2_4249 at 4943.229 kb on + strand, within Pf6N2E2_4249 at 4943.229 kb on + strand, within Pf6N2E2_4249 at 4943.230 kb on - strand, within Pf6N2E2_4249 at 4943.230 kb on - strand, within Pf6N2E2_4249 at 4943.230 kb on - strand, within Pf6N2E2_4249 at 4943.230 kb on - strand, within Pf6N2E2_4249 at 4943.230 kb on - strand, within Pf6N2E2_4249 at 4943.232 kb on + strand, within Pf6N2E2_4249 at 4943.404 kb on + strand, within Pf6N2E2_4249 at 4943.405 kb on - strand, within Pf6N2E2_4249 at 4943.405 kb on - strand, within Pf6N2E2_4249 at 4943.713 kb on + strand, within Pf6N2E2_4249 at 4943.713 kb on + strand, within Pf6N2E2_4249 at 4943.713 kb on + strand, within Pf6N2E2_4249 at 4943.713 kb on + strand, within Pf6N2E2_4249 at 4943.714 kb on - strand, within Pf6N2E2_4249 at 4943.714 kb on - strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.817 kb on + strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.818 kb on - strand, within Pf6N2E2_4249 at 4943.844 kb on + strand at 4943.844 kb on + strand at 4943.844 kb on + strand at 4943.845 kb on - strand at 4943.845 kb on - strand at 4943.845 kb on - strand at 4943.845 kb on - strand at 4943.845 kb on - strand at 4943.950 kb on + strand at 4943.950 kb on + strand at 4943.950 kb on + strand at 4944.069 kb on + strand at 4944.161 kb on + strand, within Pf6N2E2_4250 at 4944.161 kb on + strand, within Pf6N2E2_4250 at 4944.162 kb on - strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.167 kb on + strand, within Pf6N2E2_4250 at 4944.168 kb on - strand, within Pf6N2E2_4250 at 4944.168 kb on - strand, within Pf6N2E2_4250 at 4944.168 kb on - strand, within Pf6N2E2_4250 at 4944.168 kb on - strand, within Pf6N2E2_4250 at 4944.168 kb on - strand, within Pf6N2E2_4250 at 4944.168 kb on - strand, within Pf6N2E2_4250 at 4944.185 kb on + strand, within Pf6N2E2_4250 at 4944.185 kb on + strand, within Pf6N2E2_4250 at 4944.185 kb on + strand, within Pf6N2E2_4250 at 4944.185 kb on + strand, within Pf6N2E2_4250 at 4944.185 kb on + strand, within Pf6N2E2_4250 at 4944.185 kb on + strand, within Pf6N2E2_4250 at 4944.186 kb on - strand, within Pf6N2E2_4250 at 4944.186 kb on - strand, within Pf6N2E2_4250 at 4944.186 kb on - strand, within Pf6N2E2_4250 at 4944.323 kb on + strand, within Pf6N2E2_4250 at 4944.323 kb on + strand, within Pf6N2E2_4250 at 4944.323 kb on + strand, within Pf6N2E2_4250 at 4944.323 kb on + strand, within Pf6N2E2_4250 at 4944.323 kb on + strand, within Pf6N2E2_4250 at 4944.323 kb on + strand, within Pf6N2E2_4250 at 4944.323 kb on + strand, within Pf6N2E2_4250 at 4944.324 kb on - strand, within Pf6N2E2_4250 at 4944.324 kb on - strand, within Pf6N2E2_4250 at 4944.324 kb on - strand, within Pf6N2E2_4250 at 4944.440 kb on + strand, within Pf6N2E2_4250 at 4944.440 kb on + strand, within Pf6N2E2_4250 at 4944.440 kb on + strand, within Pf6N2E2_4250 at 4944.441 kb on - strand, within Pf6N2E2_4250 at 4944.441 kb on - strand, within Pf6N2E2_4250 at 4944.441 kb on - strand, within Pf6N2E2_4250 at 4944.441 kb on - strand, within Pf6N2E2_4250 at 4944.441 kb on - strand, within Pf6N2E2_4250 at 4944.617 kb on + strand, within Pf6N2E2_4250 at 4944.618 kb on - strand, within Pf6N2E2_4250 at 4944.618 kb on - strand, within Pf6N2E2_4250 at 4944.653 kb on + strand, within Pf6N2E2_4250 at 4944.653 kb on + strand, within Pf6N2E2_4250 at 4944.653 kb on + strand, within Pf6N2E2_4250 at 4944.654 kb on - strand, within Pf6N2E2_4250 at 4944.654 kb on - strand, within Pf6N2E2_4250 at 4944.654 kb on - strand, within Pf6N2E2_4250 at 4944.689 kb on + strand, within Pf6N2E2_4250 at 4944.689 kb on + strand, within Pf6N2E2_4250 at 4944.791 kb on + strand, within Pf6N2E2_4250 at 4944.791 kb on + strand, within Pf6N2E2_4250 at 4944.791 kb on + strand, within Pf6N2E2_4250 at 4944.792 kb on - strand, within Pf6N2E2_4250 at 4944.792 kb on - strand, within Pf6N2E2_4250 at 4944.917 kb on + strand, within Pf6N2E2_4250 at 4944.917 kb on + strand, within Pf6N2E2_4250 at 4944.917 kb on + strand, within Pf6N2E2_4250 at 4944.918 kb on - strand, within Pf6N2E2_4250 at 4944.918 kb on - strand, within Pf6N2E2_4250 at 4944.918 kb on - strand, within Pf6N2E2_4250 at 4944.932 kb on + strand, within Pf6N2E2_4250 at 4944.933 kb on - strand, within Pf6N2E2_4250
Per-strain Table
Position Strand Gene LocusTag Fraction rubidium chloride 0.25 M remove 4,941,856 + +1.0 4,941,856 + +0.6 4,941,856 + +0.0 4,941,856 + -0.9 4,941,856 + +0.3 4,941,856 + -1.1 4,941,857 - +0.6 4,941,857 - -1.4 4,941,857 - +1.4 4,941,857 - -0.9 4,941,857 - -0.9 4,941,857 - +1.4 4,941,857 - +0.3 4,941,857 - +0.3 4,941,857 - -0.5 4,941,857 - +0.1 4,942,212 - Pf6N2E2_4248 0.52 -0.5 4,942,237 + Pf6N2E2_4248 0.55 +0.6 4,942,237 + Pf6N2E2_4248 0.55 -0.6 4,942,237 + Pf6N2E2_4248 0.55 -1.2 4,942,237 + Pf6N2E2_4248 0.55 -0.6 4,942,238 - Pf6N2E2_4248 0.55 -2.5 4,942,301 - Pf6N2E2_4248 0.63 +0.6 4,942,374 + Pf6N2E2_4248 0.72 -1.1 4,942,374 + Pf6N2E2_4248 0.72 +0.2 4,942,374 + Pf6N2E2_4248 0.72 -0.3 4,942,374 + Pf6N2E2_4248 0.72 -0.5 4,942,375 - Pf6N2E2_4248 0.72 -0.7 4,942,396 + Pf6N2E2_4248 0.75 +0.2 4,942,406 - Pf6N2E2_4248 0.76 -0.1 4,942,406 - Pf6N2E2_4248 0.76 -0.1 4,942,576 - +0.3 4,942,576 - -1.7 4,942,576 - -0.6 4,942,576 - -0.6 4,942,585 - +0.9 4,942,604 - -0.1 4,942,727 + -0.3 4,942,742 + -0.8 4,942,852 + -0.5 4,942,852 + -0.4 4,942,852 + +1.0 4,942,852 + -0.3 4,942,852 + -2.0 4,942,852 + +0.5 4,942,852 + -0.7 4,942,852 + +0.1 4,942,852 + -1.2 4,942,853 - -0.4 4,942,853 - -0.9 4,942,853 - -0.2 4,942,853 - -0.5 4,943,132 + Pf6N2E2_4249 0.31 -1.0 4,943,229 + Pf6N2E2_4249 0.39 +1.9 4,943,229 + Pf6N2E2_4249 0.39 -1.5 4,943,230 - Pf6N2E2_4249 0.39 +1.1 4,943,230 - Pf6N2E2_4249 0.39 -1.2 4,943,230 - Pf6N2E2_4249 0.39 -0.3 4,943,230 - Pf6N2E2_4249 0.39 -0.2 4,943,230 - Pf6N2E2_4249 0.39 +1.7 4,943,232 + Pf6N2E2_4249 0.40 -0.1 4,943,404 + Pf6N2E2_4249 0.54 +0.9 4,943,405 - Pf6N2E2_4249 0.54 -0.0 4,943,405 - Pf6N2E2_4249 0.54 -0.6 4,943,713 + Pf6N2E2_4249 0.80 -1.3 4,943,713 + Pf6N2E2_4249 0.80 -0.3 4,943,713 + Pf6N2E2_4249 0.80 -1.8 4,943,713 + Pf6N2E2_4249 0.80 +0.2 4,943,714 - Pf6N2E2_4249 0.80 +0.1 4,943,714 - Pf6N2E2_4249 0.80 +0.5 4,943,817 + Pf6N2E2_4249 0.89 -0.5 4,943,817 + Pf6N2E2_4249 0.89 -1.5 4,943,817 + Pf6N2E2_4249 0.89 -1.0 4,943,817 + Pf6N2E2_4249 0.89 -1.2 4,943,817 + Pf6N2E2_4249 0.89 -0.2 4,943,817 + Pf6N2E2_4249 0.89 -1.6 4,943,817 + Pf6N2E2_4249 0.89 -0.3 4,943,817 + Pf6N2E2_4249 0.89 -0.6 4,943,817 + Pf6N2E2_4249 0.89 -0.4 4,943,817 + Pf6N2E2_4249 0.89 -1.2 4,943,817 + Pf6N2E2_4249 0.89 -0.9 4,943,817 + Pf6N2E2_4249 0.89 -1.3 4,943,817 + Pf6N2E2_4249 0.89 +0.6 4,943,818 - Pf6N2E2_4249 0.89 +0.9 4,943,818 - Pf6N2E2_4249 0.89 -0.0 4,943,818 - Pf6N2E2_4249 0.89 -0.1 4,943,818 - Pf6N2E2_4249 0.89 -0.6 4,943,818 - Pf6N2E2_4249 0.89 +0.4 4,943,818 - Pf6N2E2_4249 0.89 +0.0 4,943,818 - Pf6N2E2_4249 0.89 -1.6 4,943,818 - Pf6N2E2_4249 0.89 -0.7 4,943,818 - Pf6N2E2_4249 0.89 +0.0 4,943,818 - Pf6N2E2_4249 0.89 +0.0 4,943,818 - Pf6N2E2_4249 0.89 -0.3 4,943,818 - Pf6N2E2_4249 0.89 +0.8 4,943,818 - Pf6N2E2_4249 0.89 -0.1 4,943,818 - Pf6N2E2_4249 0.89 +0.0 4,943,844 + +0.3 4,943,844 + -0.1 4,943,844 + -1.6 4,943,845 - +0.4 4,943,845 - +0.4 4,943,845 - +0.6 4,943,845 - -0.7 4,943,845 - +0.6 4,943,950 + -2.1 4,943,950 + -1.8 4,943,950 + -0.2 4,944,069 + -0.8 4,944,161 + Pf6N2E2_4250 0.10 -0.6 4,944,161 + Pf6N2E2_4250 0.10 +0.2 4,944,162 - Pf6N2E2_4250 0.10 +0.7 4,944,167 + Pf6N2E2_4250 0.11 -0.8 4,944,167 + Pf6N2E2_4250 0.11 -0.4 4,944,167 + Pf6N2E2_4250 0.11 +0.7 4,944,167 + Pf6N2E2_4250 0.11 -0.1 4,944,167 + Pf6N2E2_4250 0.11 +1.7 4,944,167 + Pf6N2E2_4250 0.11 -1.0 4,944,167 + Pf6N2E2_4250 0.11 +0.8 4,944,167 + Pf6N2E2_4250 0.11 -0.4 4,944,167 + Pf6N2E2_4250 0.11 -0.5 4,944,167 + Pf6N2E2_4250 0.11 -1.3 4,944,168 - Pf6N2E2_4250 0.11 +0.9 4,944,168 - Pf6N2E2_4250 0.11 +0.4 4,944,168 - Pf6N2E2_4250 0.11 -0.2 4,944,168 - Pf6N2E2_4250 0.11 -1.1 4,944,168 - Pf6N2E2_4250 0.11 -0.0 4,944,168 - Pf6N2E2_4250 0.11 +0.5 4,944,185 + Pf6N2E2_4250 0.11 -0.1 4,944,185 + Pf6N2E2_4250 0.11 -0.3 4,944,185 + Pf6N2E2_4250 0.11 -0.6 4,944,185 + Pf6N2E2_4250 0.11 -0.1 4,944,185 + Pf6N2E2_4250 0.11 -1.7 4,944,185 + Pf6N2E2_4250 0.11 +0.2 4,944,186 - Pf6N2E2_4250 0.11 +1.4 4,944,186 - Pf6N2E2_4250 0.11 -0.2 4,944,186 - Pf6N2E2_4250 0.11 -0.2 4,944,323 + Pf6N2E2_4250 0.18 +0.2 4,944,323 + Pf6N2E2_4250 0.18 +0.1 4,944,323 + Pf6N2E2_4250 0.18 +1.1 4,944,323 + Pf6N2E2_4250 0.18 -0.7 4,944,323 + Pf6N2E2_4250 0.18 +0.7 4,944,323 + Pf6N2E2_4250 0.18 -0.0 4,944,323 + Pf6N2E2_4250 0.18 -0.8 4,944,324 - Pf6N2E2_4250 0.18 -0.5 4,944,324 - Pf6N2E2_4250 0.18 -2.3 4,944,324 - Pf6N2E2_4250 0.18 -1.6 4,944,440 + Pf6N2E2_4250 0.23 -1.7 4,944,440 + Pf6N2E2_4250 0.23 -0.7 4,944,440 + Pf6N2E2_4250 0.23 -0.8 4,944,441 - Pf6N2E2_4250 0.23 -0.2 4,944,441 - Pf6N2E2_4250 0.23 +0.0 4,944,441 - Pf6N2E2_4250 0.23 -0.1 4,944,441 - Pf6N2E2_4250 0.23 -1.9 4,944,441 - Pf6N2E2_4250 0.23 +0.4 4,944,617 + Pf6N2E2_4250 0.32 -1.0 4,944,618 - Pf6N2E2_4250 0.32 -0.8 4,944,618 - Pf6N2E2_4250 0.32 +0.2 4,944,653 + Pf6N2E2_4250 0.33 +0.2 4,944,653 + Pf6N2E2_4250 0.33 +0.7 4,944,653 + Pf6N2E2_4250 0.33 -0.2 4,944,654 - Pf6N2E2_4250 0.33 -1.0 4,944,654 - Pf6N2E2_4250 0.33 -0.4 4,944,654 - Pf6N2E2_4250 0.33 +0.8 4,944,689 + Pf6N2E2_4250 0.35 +2.4 4,944,689 + Pf6N2E2_4250 0.35 -0.0 4,944,791 + Pf6N2E2_4250 0.40 +0.2 4,944,791 + Pf6N2E2_4250 0.40 -1.4 4,944,791 + Pf6N2E2_4250 0.40 -0.8 4,944,792 - Pf6N2E2_4250 0.40 -0.9 4,944,792 - Pf6N2E2_4250 0.40 -0.2 4,944,917 + Pf6N2E2_4250 0.45 -0.1 4,944,917 + Pf6N2E2_4250 0.45 +0.5 4,944,917 + Pf6N2E2_4250 0.45 +0.3 4,944,918 - Pf6N2E2_4250 0.46 -1.4 4,944,918 - Pf6N2E2_4250 0.46 -0.4 4,944,918 - Pf6N2E2_4250 0.46 -0.4 4,944,932 + Pf6N2E2_4250 0.46 -0.0 4,944,933 - Pf6N2E2_4250 0.46 -0.2
Or see this region's nucleotide sequence