Experiment: rubidium chloride 0.25 M 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_2316 and Pf6N2E2_2317 are separated by 11 nucleotides Pf6N2E2_2317 and Pf6N2E2_2318 are separated by 179 nucleotides Pf6N2E2_2318 and Pf6N2E2_2319 are separated by 24 nucleotides Pf6N2E2_2319 and Pf6N2E2_2320 overlap by 8 nucleotides 
        Pf6N2E2_2316 - Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation, at 2,738,597 to 2,740,012 
        _2316 
         
        
        Pf6N2E2_2317 - Putative analog of CcoH, COG3198, at 2,740,024 to 2,740,563 
        _2317 
         
        
        Pf6N2E2_2318 - Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,740,743 to 2,743,193 
        _2318 
         
        
        Pf6N2E2_2319 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 2,743,218 to 2,743,436 
        _2319 
         
        
        Pf6N2E2_2320 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 2,743,429 to 2,744,112 
        _2320 
         Position (kb) 2740 
2741 
2742 
2743 
2744 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 
3 at 2739.787 kb on + strand, within Pf6N2E2_2316 at 2739.787 kb on + strand, within Pf6N2E2_2316 at 2739.787 kb on + strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.869 kb on + strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.966 kb on + strand at 2739.966 kb on + strand at 2739.967 kb on - strand at 2739.967 kb on - strand at 2740.054 kb on + strand at 2740.054 kb on + strand at 2740.055 kb on - strand at 2740.055 kb on - strand at 2740.055 kb on - strand at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.169 kb on - strand, within Pf6N2E2_2317 at 2740.169 kb on - strand, within Pf6N2E2_2317 at 2740.171 kb on + strand, within Pf6N2E2_2317 at 2740.171 kb on + strand, within Pf6N2E2_2317 at 2740.172 kb on - strand, within Pf6N2E2_2317 at 2740.263 kb on + strand, within Pf6N2E2_2317 at 2740.561 kb on + strand at 2740.561 kb on + strand at 2740.561 kb on + strand at 2740.625 kb on + strand at 2740.625 kb on + strand at 2740.625 kb on + strand at 2740.764 kb on + strand at 2740.893 kb on + strand at 2740.896 kb on + strand at 2740.896 kb on + strand at 2740.897 kb on - strand at 2740.897 kb on - strand at 2740.968 kb on + strand at 2740.968 kb on + strand at 2740.968 kb on + strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2741.086 kb on + strand, within Pf6N2E2_2318 at 2741.086 kb on + strand, within Pf6N2E2_2318 at 2741.087 kb on - strand, within Pf6N2E2_2318 at 2741.087 kb on - strand, within Pf6N2E2_2318 at 2741.146 kb on + strand, within Pf6N2E2_2318 at 2741.146 kb on + strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.424 kb on + strand at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.426 kb on + strand, within Pf6N2E2_2318 at 2741.520 kb on + strand, within Pf6N2E2_2318 at 2741.520 kb on + strand, within Pf6N2E2_2318 at 2741.520 kb on + strand, within Pf6N2E2_2318 at 2741.547 kb on - strand, within Pf6N2E2_2318 at 2741.566 kb on - strand, within Pf6N2E2_2318 at 2741.566 kb on - strand, within Pf6N2E2_2318 at 2741.578 kb on + strand, within Pf6N2E2_2318 at 2741.844 kb on + strand, within Pf6N2E2_2318 at 2742.392 kb on + strand, within Pf6N2E2_2318 at 2742.392 kb on + strand, within Pf6N2E2_2318 at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.062 kb on - strand at 2743.334 kb on + strand, within Pf6N2E2_2319 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.606 kb on + strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.717 kb on + strand, within Pf6N2E2_2320 at 2743.718 kb on - strand, within Pf6N2E2_2320  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction rubidium chloride 0.25 M remove 2,739,787 +  Pf6N2E2_2316 0.84  +0.2 2,739,787 +  Pf6N2E2_2316 0.84  -2.1 2,739,787 +  Pf6N2E2_2316 0.84  -0.2 2,739,788 -  Pf6N2E2_2316 0.84  -2.0 2,739,788 -  Pf6N2E2_2316 0.84  +0.2 2,739,788 -  Pf6N2E2_2316 0.84  +0.0 2,739,788 -  Pf6N2E2_2316 0.84  -0.8 2,739,788 -  Pf6N2E2_2316 0.84  -0.2 2,739,788 -  Pf6N2E2_2316 0.84  -0.6 2,739,791 +  Pf6N2E2_2316 0.84  -0.5 2,739,791 +  Pf6N2E2_2316 0.84  +0.2 2,739,791 +  Pf6N2E2_2316 0.84  +0.3 2,739,791 +  Pf6N2E2_2316 0.84  -1.1 2,739,791 +  Pf6N2E2_2316 0.84  -0.2 2,739,791 +  Pf6N2E2_2316 0.84  +3.0 2,739,791 +  Pf6N2E2_2316 0.84  +1.1 2,739,791 +  Pf6N2E2_2316 0.84  +1.0 2,739,791 +  Pf6N2E2_2316 0.84  +0.4 2,739,791 +  Pf6N2E2_2316 0.84  -1.0 2,739,792 -  Pf6N2E2_2316 0.84  -0.3 2,739,792 -  Pf6N2E2_2316 0.84  +0.5 2,739,792 -  Pf6N2E2_2316 0.84  -2.9 2,739,792 -  Pf6N2E2_2316 0.84  -0.2 2,739,792 -  Pf6N2E2_2316 0.84  +0.0 2,739,792 -  Pf6N2E2_2316 0.84  -1.2 2,739,792 -  Pf6N2E2_2316 0.84  -0.8 2,739,792 -  Pf6N2E2_2316 0.84  +1.0 2,739,869 +  Pf6N2E2_2316 0.90  -0.3 2,739,870 -  Pf6N2E2_2316 0.90  -0.7 2,739,870 -  Pf6N2E2_2316 0.90  -0.7 2,739,870 -  Pf6N2E2_2316 0.90  -1.5 2,739,870 -  Pf6N2E2_2316 0.90  -1.5 2,739,966 +  -0.2 2,739,966 +  -0.8 2,739,967 -  -1.5 2,739,967 -  -0.2 2,740,054 +  +0.5 2,740,054 +  +2.6 2,740,055 -  +1.3 2,740,055 -  -1.5 2,740,055 -  -1.7 2,740,168 +  Pf6N2E2_2317 0.27  +0.9 2,740,168 +  Pf6N2E2_2317 0.27  -0.8 2,740,168 +  Pf6N2E2_2317 0.27  -0.5 2,740,168 +  Pf6N2E2_2317 0.27  -0.7 2,740,169 -  Pf6N2E2_2317 0.27  -2.1 2,740,169 -  Pf6N2E2_2317 0.27  -1.0 2,740,171 +  Pf6N2E2_2317 0.27  +1.2 2,740,171 +  Pf6N2E2_2317 0.27  +1.3 2,740,172 -  Pf6N2E2_2317 0.27  -0.8 2,740,263 +  Pf6N2E2_2317 0.44  -1.2 2,740,561 +  +1.6 2,740,561 +  +0.4 2,740,561 +  +0.3 2,740,625 +  +0.9 2,740,625 +  -0.5 2,740,625 +  +0.4 2,740,764 +  -1.5 2,740,893 +  -1.8 2,740,896 +  +0.3 2,740,896 +  +0.0 2,740,897 -  -2.1 2,740,897 -  -0.4 2,740,968 +  +0.0 2,740,968 +  +0.6 2,740,968 +  -0.4 2,740,969 -  -1.0 2,740,969 -  -0.7 2,740,969 -  +1.6 2,740,969 -  -1.2 2,740,969 -  +1.0 2,741,086 +  Pf6N2E2_2318 0.14  -0.8 2,741,086 +  Pf6N2E2_2318 0.14  +0.0 2,741,087 -  Pf6N2E2_2318 0.14  -1.5 2,741,087 -  Pf6N2E2_2318 0.14  +0.6 2,741,146 +  Pf6N2E2_2318 0.16  -2.0 2,741,146 +  Pf6N2E2_2318 0.16  +1.2 2,741,147 -  Pf6N2E2_2318 0.16  -0.7 2,741,147 -  Pf6N2E2_2318 0.16  -0.2 2,741,147 -  Pf6N2E2_2318 0.16  -1.6 2,741,147 -  Pf6N2E2_2318 0.16  -3.0 2,741,424 +  Pf6N2E2_2318 0.28  -0.7 2,741,424 +  +1.6 2,741,424 +  Pf6N2E2_2318 0.28  -2.1 2,741,424 +  Pf6N2E2_2318 0.28  -0.7 2,741,424 +  Pf6N2E2_2318 0.28  -0.3 2,741,425 -  Pf6N2E2_2318 0.28  -2.5 2,741,425 -  Pf6N2E2_2318 0.28  -1.4 2,741,425 -  Pf6N2E2_2318 0.28  -2.5 2,741,425 -  Pf6N2E2_2318 0.28  -1.1 2,741,425 -  Pf6N2E2_2318 0.28  -1.1 2,741,425 -  Pf6N2E2_2318 0.28  -0.4 2,741,426 +  Pf6N2E2_2318 0.28  -1.0 2,741,520 +  Pf6N2E2_2318 0.32  -0.3 2,741,520 +  Pf6N2E2_2318 0.32  -0.5 2,741,520 +  Pf6N2E2_2318 0.32  -1.0 2,741,547 -  Pf6N2E2_2318 0.33  +0.8 2,741,566 -  Pf6N2E2_2318 0.34  -0.5 2,741,566 -  Pf6N2E2_2318 0.34  -1.4 2,741,578 +  Pf6N2E2_2318 0.34  -1.7 2,741,844 +  Pf6N2E2_2318 0.45  -1.0 2,742,392 +  Pf6N2E2_2318 0.67  -1.5 2,742,392 +  Pf6N2E2_2318 0.67  +0.0 2,743,020 +  -2.7 2,743,020 +  -0.6 2,743,020 +  -0.1 2,743,021 -  +1.6 2,743,021 -  -0.4 2,743,021 -  +1.6 2,743,021 -  -0.1 2,743,062 -  -0.4 2,743,334 +  Pf6N2E2_2319 0.53  -0.4 2,743,582 +  Pf6N2E2_2320 0.22  -0.7 2,743,582 +  Pf6N2E2_2320 0.22  +0.6 2,743,606 +  Pf6N2E2_2320 0.26  +0.0 2,743,607 -  Pf6N2E2_2320 0.26  -2.1 2,743,607 -  Pf6N2E2_2320 0.26  -1.5 2,743,717 +  Pf6N2E2_2320 0.42  -0.7 2,743,718 -  Pf6N2E2_2320 0.42  -0.1 
Or see this region's nucleotide sequence