Experiment: rubidium chloride 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1330 and Pf6N2E2_1331 are separated by 17 nucleotides Pf6N2E2_1331 and Pf6N2E2_1332 are separated by 12 nucleotides
Pf6N2E2_1330 - Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases, at 1,565,010 to 1,566,467
_1330
Pf6N2E2_1331 - General substrate transporter:Major facilitator superfamily, at 1,566,485 to 1,567,741
_1331
Pf6N2E2_1332 - Protein involved in meta-pathway of phenol degradation, at 1,567,754 to 1,568,686
_1332
Position (kb)
1566
1567
1568 Strain fitness (log2 ratio)
-2
-1
0
1 at 1565.497 kb on + strand, within Pf6N2E2_1330 at 1565.498 kb on - strand, within Pf6N2E2_1330 at 1565.646 kb on - strand, within Pf6N2E2_1330 at 1565.646 kb on - strand, within Pf6N2E2_1330 at 1565.717 kb on - strand, within Pf6N2E2_1330 at 1565.810 kb on + strand, within Pf6N2E2_1330 at 1566.462 kb on - strand at 1566.482 kb on - strand at 1566.482 kb on - strand at 1566.669 kb on + strand, within Pf6N2E2_1331 at 1566.669 kb on + strand, within Pf6N2E2_1331 at 1566.670 kb on - strand, within Pf6N2E2_1331 at 1566.670 kb on - strand, within Pf6N2E2_1331 at 1566.670 kb on - strand, within Pf6N2E2_1331 at 1566.682 kb on + strand, within Pf6N2E2_1331 at 1566.808 kb on - strand, within Pf6N2E2_1331 at 1566.808 kb on - strand, within Pf6N2E2_1331 at 1567.194 kb on + strand, within Pf6N2E2_1331 at 1567.195 kb on - strand, within Pf6N2E2_1331 at 1567.195 kb on - strand, within Pf6N2E2_1331 at 1567.323 kb on + strand, within Pf6N2E2_1331 at 1567.324 kb on - strand, within Pf6N2E2_1331 at 1567.447 kb on - strand, within Pf6N2E2_1331 at 1567.755 kb on + strand at 1567.772 kb on - strand at 1567.786 kb on + strand at 1567.824 kb on + strand at 1567.824 kb on + strand at 1567.824 kb on + strand at 1567.825 kb on - strand at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.863 kb on + strand, within Pf6N2E2_1332 at 1567.864 kb on - strand, within Pf6N2E2_1332 at 1567.864 kb on - strand, within Pf6N2E2_1332 at 1567.948 kb on - strand, within Pf6N2E2_1332 at 1567.950 kb on + strand, within Pf6N2E2_1332 at 1567.950 kb on + strand, within Pf6N2E2_1332 at 1567.951 kb on - strand, within Pf6N2E2_1332 at 1568.031 kb on + strand, within Pf6N2E2_1332 at 1568.031 kb on + strand, within Pf6N2E2_1332 at 1568.032 kb on - strand, within Pf6N2E2_1332 at 1568.106 kb on + strand, within Pf6N2E2_1332 at 1568.107 kb on - strand, within Pf6N2E2_1332 at 1568.107 kb on - strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.373 kb on + strand, within Pf6N2E2_1332 at 1568.374 kb on - strand, within Pf6N2E2_1332 at 1568.412 kb on + strand, within Pf6N2E2_1332 at 1568.412 kb on + strand, within Pf6N2E2_1332 at 1568.413 kb on - strand, within Pf6N2E2_1332 at 1568.413 kb on - strand, within Pf6N2E2_1332 at 1568.413 kb on - strand, within Pf6N2E2_1332 at 1568.497 kb on - strand, within Pf6N2E2_1332 at 1568.497 kb on - strand, within Pf6N2E2_1332 at 1568.506 kb on + strand, within Pf6N2E2_1332 at 1568.507 kb on - strand, within Pf6N2E2_1332 at 1568.507 kb on - strand, within Pf6N2E2_1332 at 1568.507 kb on - strand, within Pf6N2E2_1332 at 1568.565 kb on + strand, within Pf6N2E2_1332 at 1568.565 kb on + strand, within Pf6N2E2_1332 at 1568.566 kb on - strand, within Pf6N2E2_1332 at 1568.651 kb on - strand at 1568.651 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rubidium chloride 0.25 M remove 1,565,497 + Pf6N2E2_1330 0.33 +0.2 1,565,498 - Pf6N2E2_1330 0.33 -0.0 1,565,646 - Pf6N2E2_1330 0.44 -0.6 1,565,646 - Pf6N2E2_1330 0.44 -1.1 1,565,717 - Pf6N2E2_1330 0.48 +0.8 1,565,810 + Pf6N2E2_1330 0.55 +0.1 1,566,462 - +0.2 1,566,482 - +0.3 1,566,482 - +1.0 1,566,669 + Pf6N2E2_1331 0.15 -0.5 1,566,669 + Pf6N2E2_1331 0.15 -0.1 1,566,670 - Pf6N2E2_1331 0.15 -2.0 1,566,670 - Pf6N2E2_1331 0.15 +1.2 1,566,670 - Pf6N2E2_1331 0.15 -1.3 1,566,682 + Pf6N2E2_1331 0.16 -0.1 1,566,808 - Pf6N2E2_1331 0.26 +0.2 1,566,808 - Pf6N2E2_1331 0.26 +0.2 1,567,194 + Pf6N2E2_1331 0.56 -1.2 1,567,195 - Pf6N2E2_1331 0.56 -1.2 1,567,195 - Pf6N2E2_1331 0.56 -2.1 1,567,323 + Pf6N2E2_1331 0.67 -1.3 1,567,324 - Pf6N2E2_1331 0.67 -1.0 1,567,447 - Pf6N2E2_1331 0.77 +0.4 1,567,755 + -0.7 1,567,772 - -0.8 1,567,786 + -0.9 1,567,824 + -1.6 1,567,824 + -0.1 1,567,824 + +0.3 1,567,825 - +0.1 1,567,863 + Pf6N2E2_1332 0.12 +0.8 1,567,863 + Pf6N2E2_1332 0.12 +0.1 1,567,863 + Pf6N2E2_1332 0.12 +0.7 1,567,863 + Pf6N2E2_1332 0.12 -1.8 1,567,864 - Pf6N2E2_1332 0.12 -0.1 1,567,864 - Pf6N2E2_1332 0.12 -0.8 1,567,948 - Pf6N2E2_1332 0.21 -1.8 1,567,950 + Pf6N2E2_1332 0.21 +0.9 1,567,950 + Pf6N2E2_1332 0.21 -0.9 1,567,951 - Pf6N2E2_1332 0.21 -0.8 1,568,031 + Pf6N2E2_1332 0.30 +0.0 1,568,031 + Pf6N2E2_1332 0.30 +0.2 1,568,032 - Pf6N2E2_1332 0.30 +0.5 1,568,106 + Pf6N2E2_1332 0.38 +0.2 1,568,107 - Pf6N2E2_1332 0.38 -0.1 1,568,107 - Pf6N2E2_1332 0.38 +0.8 1,568,373 + Pf6N2E2_1332 0.66 +1.2 1,568,373 + Pf6N2E2_1332 0.66 -0.0 1,568,373 + Pf6N2E2_1332 0.66 +0.6 1,568,373 + Pf6N2E2_1332 0.66 +0.5 1,568,373 + Pf6N2E2_1332 0.66 +0.7 1,568,374 - Pf6N2E2_1332 0.66 -0.6 1,568,412 + Pf6N2E2_1332 0.71 -0.1 1,568,412 + Pf6N2E2_1332 0.71 +0.2 1,568,413 - Pf6N2E2_1332 0.71 +0.1 1,568,413 - Pf6N2E2_1332 0.71 +0.7 1,568,413 - Pf6N2E2_1332 0.71 +0.4 1,568,497 - Pf6N2E2_1332 0.80 -0.8 1,568,497 - Pf6N2E2_1332 0.80 +0.0 1,568,506 + Pf6N2E2_1332 0.81 -0.1 1,568,507 - Pf6N2E2_1332 0.81 +0.0 1,568,507 - Pf6N2E2_1332 0.81 -0.1 1,568,507 - Pf6N2E2_1332 0.81 -0.1 1,568,565 + Pf6N2E2_1332 0.87 -1.3 1,568,565 + Pf6N2E2_1332 0.87 +1.2 1,568,566 - Pf6N2E2_1332 0.87 -0.2 1,568,651 - +1.2 1,568,651 - -1.3
Or see this region's nucleotide sequence