Experiment: rubidium chloride 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1025 and Pf6N2E2_1026 are separated by 99 nucleotides Pf6N2E2_1026 and Pf6N2E2_1027 overlap by 4 nucleotides Pf6N2E2_1027 and Pf6N2E2_1028 are separated by 195 nucleotides
Pf6N2E2_1025 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,198,427 to 1,199,380
_1025
Pf6N2E2_1026 - Rhodanese-related sulfurtransferase, at 1,199,480 to 1,201,063
_1026
Pf6N2E2_1027 - Cysteine dioxygenase (EC 1.13.11.20), at 1,201,060 to 1,201,695
_1027
Pf6N2E2_1028 - Transcriptional regulator, LysR family, at 1,201,891 to 1,202,796
_1028
Position (kb)
1199
1200
1201
1202 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1198.527 kb on + strand, within Pf6N2E2_1025 at 1198.528 kb on - strand, within Pf6N2E2_1025 at 1198.528 kb on - strand, within Pf6N2E2_1025 at 1198.736 kb on - strand, within Pf6N2E2_1025 at 1198.767 kb on + strand, within Pf6N2E2_1025 at 1198.767 kb on + strand, within Pf6N2E2_1025 at 1198.974 kb on + strand, within Pf6N2E2_1025 at 1199.071 kb on + strand, within Pf6N2E2_1025 at 1199.072 kb on - strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.140 kb on - strand, within Pf6N2E2_1025 at 1199.140 kb on - strand, within Pf6N2E2_1025 at 1199.446 kb on + strand at 1199.446 kb on + strand at 1199.535 kb on + strand at 1199.535 kb on + strand at 1199.536 kb on - strand at 1199.536 kb on - strand at 1199.550 kb on + strand at 1199.589 kb on + strand at 1199.590 kb on - strand at 1199.601 kb on + strand at 1199.772 kb on + strand, within Pf6N2E2_1026 at 1199.772 kb on + strand, within Pf6N2E2_1026 at 1199.773 kb on - strand, within Pf6N2E2_1026 at 1199.773 kb on - strand, within Pf6N2E2_1026 at 1199.859 kb on + strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1200.021 kb on + strand, within Pf6N2E2_1026 at 1200.021 kb on + strand, within Pf6N2E2_1026 at 1200.021 kb on + strand, within Pf6N2E2_1026 at 1200.022 kb on - strand, within Pf6N2E2_1026 at 1200.022 kb on - strand, within Pf6N2E2_1026 at 1200.022 kb on - strand, within Pf6N2E2_1026 at 1200.219 kb on + strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.686 kb on - strand, within Pf6N2E2_1026 at 1200.808 kb on - strand, within Pf6N2E2_1026 at 1200.808 kb on - strand, within Pf6N2E2_1026 at 1200.808 kb on - strand, within Pf6N2E2_1026 at 1200.903 kb on + strand, within Pf6N2E2_1026 at 1201.052 kb on + strand at 1201.053 kb on - strand at 1201.064 kb on - strand at 1201.157 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.482 kb on - strand at 1201.482 kb on - strand, within Pf6N2E2_1027 at 1201.832 kb on + strand at 1201.864 kb on - strand at 1201.864 kb on - strand at 1201.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rubidium chloride 0.25 M remove 1,198,527 + Pf6N2E2_1025 0.10 -0.8 1,198,528 - Pf6N2E2_1025 0.11 +0.1 1,198,528 - Pf6N2E2_1025 0.11 +0.7 1,198,736 - Pf6N2E2_1025 0.32 +1.3 1,198,767 + Pf6N2E2_1025 0.36 +0.2 1,198,767 + Pf6N2E2_1025 0.36 -0.4 1,198,974 + Pf6N2E2_1025 0.57 -0.2 1,199,071 + Pf6N2E2_1025 0.68 +0.2 1,199,072 - Pf6N2E2_1025 0.68 -0.6 1,199,139 + Pf6N2E2_1025 0.75 -0.2 1,199,139 + Pf6N2E2_1025 0.75 +0.6 1,199,139 + Pf6N2E2_1025 0.75 -0.5 1,199,139 + Pf6N2E2_1025 0.75 +0.5 1,199,140 - Pf6N2E2_1025 0.75 +0.6 1,199,140 - Pf6N2E2_1025 0.75 +0.5 1,199,446 + +0.3 1,199,446 + -1.1 1,199,535 + -0.7 1,199,535 + -1.1 1,199,536 - +0.8 1,199,536 - +1.2 1,199,550 + +0.1 1,199,589 + +1.0 1,199,590 - +1.8 1,199,601 + -1.0 1,199,772 + Pf6N2E2_1026 0.18 +0.6 1,199,772 + Pf6N2E2_1026 0.18 -0.6 1,199,773 - Pf6N2E2_1026 0.18 -0.7 1,199,773 - Pf6N2E2_1026 0.18 +0.7 1,199,859 + Pf6N2E2_1026 0.24 -1.4 1,199,860 - Pf6N2E2_1026 0.24 -0.4 1,199,860 - Pf6N2E2_1026 0.24 +0.1 1,199,860 - Pf6N2E2_1026 0.24 +0.6 1,199,860 - Pf6N2E2_1026 0.24 +2.2 1,200,021 + Pf6N2E2_1026 0.34 -0.9 1,200,021 + Pf6N2E2_1026 0.34 -0.5 1,200,021 + Pf6N2E2_1026 0.34 -1.2 1,200,022 - Pf6N2E2_1026 0.34 +0.5 1,200,022 - Pf6N2E2_1026 0.34 +0.1 1,200,022 - Pf6N2E2_1026 0.34 -0.9 1,200,219 + Pf6N2E2_1026 0.47 -0.1 1,200,220 - Pf6N2E2_1026 0.47 -0.8 1,200,220 - Pf6N2E2_1026 0.47 -0.7 1,200,220 - Pf6N2E2_1026 0.47 +0.0 1,200,220 - Pf6N2E2_1026 0.47 -0.8 1,200,220 - Pf6N2E2_1026 0.47 +0.3 1,200,220 - Pf6N2E2_1026 0.47 -0.3 1,200,220 - Pf6N2E2_1026 0.47 +0.2 1,200,686 - Pf6N2E2_1026 0.76 -3.4 1,200,808 - Pf6N2E2_1026 0.84 -0.3 1,200,808 - Pf6N2E2_1026 0.84 -1.9 1,200,808 - Pf6N2E2_1026 0.84 -1.3 1,200,903 + Pf6N2E2_1026 0.90 +0.2 1,201,052 + -0.5 1,201,053 - -0.0 1,201,064 - -0.1 1,201,157 + Pf6N2E2_1027 0.15 +1.2 1,201,481 + Pf6N2E2_1027 0.66 +0.2 1,201,481 + Pf6N2E2_1027 0.66 -0.3 1,201,481 + Pf6N2E2_1027 0.66 +1.0 1,201,482 - +0.8 1,201,482 - Pf6N2E2_1027 0.66 +0.7 1,201,832 + +2.4 1,201,864 - -1.5 1,201,864 - +0.5 1,201,864 - +1.0
Or see this region's nucleotide sequence