Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08775 and MMP_RS08780 are separated by 9 nucleotides MMP_RS08780 and MMP_RS08785 are separated by 5 nucleotides MMP_RS08785 and MMP_RS08790 are separated by 8 nucleotides MMP_RS08790 and MMP_RS08795 are separated by 113 nucleotides MMP_RS08795 and MMP_RS08800 are separated by 22 nucleotides MMP_RS08800 and MMP_RS08805 overlap by 44 nucleotides
MMP_RS08775: MMP_RS08775 - methanogenesis marker 9 domain-containing protein, at 1,645,101 to 1,645,556
_RS08775
MMP_RS08780: MMP_RS08780 - 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase, at 1,645,566 to 1,646,231
_RS08780
MMP_RS08785: MMP_RS08785 - RNA-protein complex protein Nop10, at 1,646,237 to 1,646,392
_RS08785
MMP_RS08790: MMP_RS08790 - translation initiation factor IF-2 subunit alpha, at 1,646,401 to 1,647,198
_RS08790
MMP_RS08795: MMP_RS08795 - 30S ribosomal protein S27e, at 1,647,312 to 1,647,497
_RS08795
MMP_RS08800: MMP_RS08800 - 50S ribosomal protein L44e, at 1,647,520 to 1,647,801
_RS08800
MMP_RS08805: MMP_RS08805 - hypothetical protein, at 1,647,758 to 1,648,345
_RS08805
Position (kb)
1646
1647
1648 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1645.416 kb on - strand, within MMP_RS08775 at 1645.513 kb on + strand at 1645.556 kb on - strand at 1645.556 kb on - strand at 1645.559 kb on + strand at 1645.567 kb on - strand at 1645.740 kb on - strand at 1645.740 kb on - strand at 1645.804 kb on + strand at 1645.804 kb on + strand at 1645.838 kb on - strand at 1645.850 kb on - strand at 1645.851 kb on + strand at 1645.917 kb on - strand at 1645.919 kb on - strand at 1645.967 kb on + strand at 1645.980 kb on + strand at 1646.021 kb on + strand at 1646.072 kb on - strand at 1646.095 kb on - strand at 1646.175 kb on - strand at 1646.175 kb on - strand at 1646.231 kb on - strand at 1646.277 kb on - strand, within MMP_RS08785 at 1646.524 kb on - strand, within MMP_RS08790 at 1646.550 kb on + strand, within MMP_RS08790 at 1646.713 kb on - strand, within MMP_RS08790 at 1647.112 kb on - strand, within MMP_RS08790 at 1647.218 kb on - strand at 1647.219 kb on + strand at 1647.223 kb on - strand at 1647.224 kb on + strand at 1647.230 kb on + strand at 1647.240 kb on - strand at 1647.243 kb on + strand at 1647.250 kb on + strand at 1647.277 kb on - strand at 1647.278 kb on + strand at 1647.283 kb on - strand at 1647.304 kb on + strand at 1647.313 kb on - strand at 1647.319 kb on + strand at 1647.322 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,645,416 - MMP_RS08775 0.69 +0.6 1,645,513 + +0.3 1,645,556 - +0.0 1,645,556 - -0.0 1,645,559 + +0.7 1,645,567 - +0.2 1,645,740 - -1.9 1,645,740 - -2.0 1,645,804 + -2.1 1,645,804 + -1.8 1,645,838 - -1.5 1,645,850 - -2.7 1,645,851 + -0.7 1,645,917 - -1.2 1,645,919 - -2.8 1,645,967 + -2.7 1,645,980 + -1.4 1,646,021 + -2.3 1,646,072 - -2.2 1,646,095 - -2.9 1,646,175 - -2.1 1,646,175 - -1.6 1,646,231 - -2.1 1,646,277 - MMP_RS08785 0.26 +0.4 1,646,524 - MMP_RS08790 0.15 -0.7 1,646,550 + MMP_RS08790 0.19 -1.4 1,646,713 - MMP_RS08790 0.39 -0.4 1,647,112 - MMP_RS08790 0.89 -1.7 1,647,218 - -1.1 1,647,219 + -0.2 1,647,223 - +0.3 1,647,224 + -0.9 1,647,230 + -1.3 1,647,240 - +0.0 1,647,243 + -1.0 1,647,250 + -0.1 1,647,277 - +0.3 1,647,278 + -0.1 1,647,283 - -0.7 1,647,304 + +0.3 1,647,313 - -0.7 1,647,319 + +0.1 1,647,322 + -0.3
Or see this region's nucleotide sequence