Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05545 and MMP_RS05550 are separated by 199 nucleotides MMP_RS05550 and MMP_RS05555 are separated by 9 nucleotides MMP_RS05555 and MMP_RS05560 are separated by 70 nucleotides MMP_RS05560 and MMP_RS05565 are separated by 1 nucleotides
MMP_RS05545: MMP_RS05545 - pyridoxal phosphate-dependent aminotransferase, at 1,063,545 to 1,064,672
_RS05545
MMP_RS05550: MMP_RS05550 - cation:proton antiporter, at 1,064,872 to 1,065,126
_RS05550
MMP_RS05555: MMP_RS05555 - DUF4040 domain-containing protein, at 1,065,136 to 1,065,378
_RS05555
MMP_RS05560: MMP_RS05560 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 1,065,449 to 1,065,919
_RS05560
MMP_RS05565: MMP_RS05565 - NTP transferase domain-containing protein, at 1,065,921 to 1,067,156
_RS05565
Position (kb)
1064
1065
1066 Strain fitness (log2 ratio)
-2
-1
0
1 at 1063.943 kb on - strand, within MMP_RS05545 at 1064.087 kb on + strand, within MMP_RS05545 at 1064.684 kb on + strand at 1064.688 kb on + strand at 1064.691 kb on - strand at 1064.692 kb on + strand at 1064.694 kb on + strand at 1064.694 kb on + strand at 1064.695 kb on - strand at 1064.695 kb on - strand at 1064.695 kb on - strand at 1064.696 kb on + strand at 1064.696 kb on + strand at 1064.696 kb on + strand at 1064.696 kb on + strand at 1064.709 kb on + strand at 1064.709 kb on + strand at 1064.718 kb on + strand at 1064.751 kb on - strand at 1064.754 kb on - strand at 1064.754 kb on - strand at 1064.754 kb on - strand at 1064.755 kb on + strand at 1064.755 kb on + strand at 1064.755 kb on + strand at 1064.755 kb on + strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.762 kb on - strand at 1064.763 kb on + strand at 1064.763 kb on + strand at 1064.763 kb on + strand at 1064.777 kb on + strand at 1064.784 kb on - strand at 1064.858 kb on - strand at 1064.859 kb on + strand at 1065.006 kb on - strand, within MMP_RS05550 at 1065.213 kb on + strand, within MMP_RS05555 at 1065.276 kb on + strand, within MMP_RS05555 at 1065.376 kb on - strand at 1065.381 kb on - strand at 1065.387 kb on - strand at 1065.457 kb on - strand at 1065.457 kb on - strand at 1065.463 kb on + strand at 1065.474 kb on - strand at 1065.474 kb on - strand at 1065.474 kb on - strand at 1065.528 kb on - strand, within MMP_RS05560 at 1065.528 kb on - strand, within MMP_RS05560 at 1065.533 kb on + strand, within MMP_RS05560 at 1065.533 kb on + strand, within MMP_RS05560 at 1065.535 kb on + strand, within MMP_RS05560 at 1065.555 kb on + strand, within MMP_RS05560 at 1065.607 kb on - strand, within MMP_RS05560 at 1065.608 kb on + strand, within MMP_RS05560 at 1065.608 kb on + strand, within MMP_RS05560 at 1065.610 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.706 kb on + strand, within MMP_RS05560 at 1065.902 kb on + strand at 1065.922 kb on - strand at 1065.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,063,943 - MMP_RS05545 0.35 -1.0 1,064,087 + MMP_RS05545 0.48 -0.5 1,064,684 + -1.0 1,064,688 + -0.8 1,064,691 - -0.7 1,064,692 + -0.8 1,064,694 + -0.3 1,064,694 + -0.5 1,064,695 - -0.5 1,064,695 - -1.5 1,064,695 - +0.1 1,064,696 + -1.9 1,064,696 + -1.2 1,064,696 + -1.4 1,064,696 + -1.6 1,064,709 + -1.3 1,064,709 + -0.2 1,064,718 + -0.7 1,064,751 - -1.2 1,064,754 - -0.2 1,064,754 - -0.5 1,064,754 - +0.3 1,064,755 + +0.1 1,064,755 + -0.4 1,064,755 + -0.8 1,064,755 + -0.9 1,064,756 - -0.7 1,064,756 - -1.1 1,064,756 - -0.8 1,064,756 - -0.8 1,064,756 - +0.4 1,064,757 + -1.2 1,064,757 + -0.8 1,064,757 + -0.3 1,064,757 + -0.1 1,064,757 + -0.5 1,064,757 + -0.5 1,064,758 - -0.5 1,064,758 - -1.0 1,064,758 - -0.5 1,064,758 - -1.1 1,064,758 - -0.0 1,064,758 - -0.8 1,064,759 + -0.9 1,064,759 + -1.2 1,064,759 + +0.9 1,064,759 + -0.2 1,064,759 + -0.2 1,064,759 + -0.6 1,064,759 + -0.8 1,064,759 + -0.6 1,064,759 + -0.4 1,064,762 - -0.7 1,064,763 + -0.0 1,064,763 + -1.0 1,064,763 + +0.1 1,064,777 + +0.1 1,064,784 - -1.7 1,064,858 - -2.4 1,064,859 + -1.2 1,065,006 - MMP_RS05550 0.53 -1.1 1,065,213 + MMP_RS05555 0.32 -1.9 1,065,276 + MMP_RS05555 0.58 -1.5 1,065,376 - -0.2 1,065,381 - +0.3 1,065,387 - +0.2 1,065,457 - -0.2 1,065,457 - -0.4 1,065,463 + -0.2 1,065,474 - -0.1 1,065,474 - +0.3 1,065,474 - -0.4 1,065,528 - MMP_RS05560 0.17 +0.1 1,065,528 - MMP_RS05560 0.17 +1.2 1,065,533 + MMP_RS05560 0.18 +0.1 1,065,533 + MMP_RS05560 0.18 -0.1 1,065,535 + MMP_RS05560 0.18 +0.4 1,065,555 + MMP_RS05560 0.23 +0.3 1,065,607 - MMP_RS05560 0.34 +0.5 1,065,608 + MMP_RS05560 0.34 +0.1 1,065,608 + MMP_RS05560 0.34 +0.9 1,065,610 + MMP_RS05560 0.34 -0.1 1,065,706 + MMP_RS05560 0.55 -0.0 1,065,706 + MMP_RS05560 0.55 +0.0 1,065,706 + MMP_RS05560 0.55 +0.3 1,065,706 + MMP_RS05560 0.55 -0.1 1,065,902 + -0.5 1,065,922 - +0.2 1,065,922 - +0.1
Or see this region's nucleotide sequence