Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04700 and MMP_RS04705 are separated by 14 nucleotides MMP_RS04705 and MMP_RS04710 are separated by 29 nucleotides MMP_RS04710 and MMP_RS04715 are separated by 185 nucleotides MMP_RS04715 and MMP_RS04720 are separated by 14 nucleotides
MMP_RS04700: MMP_RS04700 - hypothetical protein, at 899,384 to 899,962
_RS04700
MMP_RS04705: MMP_RS04705 - ribonuclease Z, at 899,977 to 900,903
_RS04705
MMP_RS04710: MMP_RS04710 - hypothetical protein, at 900,933 to 901,325
_RS04710
MMP_RS04715: MMP_RS04715 - CBS domain-containing protein, at 901,511 to 901,891
_RS04715
MMP_RS04720: MMP_RS04720 - DedA family protein, at 901,906 to 902,388
_RS04720
Position (kb)
900
901
902 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 900.074 kb on + strand at 900.265 kb on - strand at 900.298 kb on - strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.299 kb on + strand at 900.323 kb on + strand at 900.464 kb on - strand at 900.465 kb on + strand at 900.501 kb on - strand at 900.913 kb on - strand at 900.914 kb on + strand at 900.914 kb on + strand at 900.914 kb on + strand at 900.916 kb on + strand at 900.939 kb on - strand at 900.940 kb on + strand at 901.094 kb on - strand, within MMP_RS04710 at 901.169 kb on - strand, within MMP_RS04710 at 901.272 kb on + strand, within MMP_RS04710 at 901.286 kb on - strand at 901.304 kb on - strand at 901.307 kb on - strand at 901.308 kb on + strand at 901.308 kb on + strand at 901.308 kb on + strand at 901.308 kb on + strand at 901.341 kb on - strand at 901.360 kb on - strand at 901.362 kb on - strand at 901.362 kb on - strand at 901.362 kb on - strand at 901.363 kb on + strand at 901.363 kb on + strand at 901.363 kb on + strand at 901.365 kb on + strand at 901.365 kb on + strand at 901.365 kb on + strand at 901.383 kb on - strand at 901.393 kb on - strand at 901.402 kb on + strand at 901.403 kb on - strand at 901.403 kb on - strand at 901.403 kb on - strand at 901.404 kb on + strand at 901.404 kb on + strand at 901.404 kb on + strand at 901.407 kb on - strand at 901.407 kb on - strand at 901.408 kb on + strand at 901.423 kb on + strand at 901.453 kb on - strand at 901.470 kb on - strand at 901.470 kb on - strand at 901.474 kb on - strand at 901.474 kb on - strand at 901.475 kb on + strand at 901.476 kb on - strand at 901.476 kb on - strand at 901.477 kb on + strand at 901.477 kb on + strand at 901.478 kb on - strand at 901.478 kb on - strand at 901.479 kb on + strand at 901.479 kb on + strand at 901.481 kb on - strand at 901.511 kb on + strand at 901.582 kb on - strand, within MMP_RS04715 at 901.583 kb on + strand, within MMP_RS04715 at 901.583 kb on + strand, within MMP_RS04715 at 901.598 kb on + strand, within MMP_RS04715 at 901.620 kb on - strand, within MMP_RS04715 at 901.626 kb on - strand, within MMP_RS04715 at 901.627 kb on + strand, within MMP_RS04715 at 901.636 kb on - strand, within MMP_RS04715 at 901.648 kb on - strand, within MMP_RS04715 at 901.649 kb on + strand, within MMP_RS04715 at 901.650 kb on - strand, within MMP_RS04715 at 901.702 kb on + strand, within MMP_RS04715 at 901.710 kb on - strand, within MMP_RS04715 at 901.710 kb on - strand, within MMP_RS04715 at 901.729 kb on - strand, within MMP_RS04715 at 901.729 kb on - strand, within MMP_RS04715 at 901.740 kb on - strand, within MMP_RS04715 at 901.750 kb on + strand, within MMP_RS04715 at 901.931 kb on + strand at 902.011 kb on + strand, within MMP_RS04720 at 902.012 kb on - strand, within MMP_RS04720 at 902.016 kb on - strand, within MMP_RS04720 at 902.017 kb on + strand, within MMP_RS04720 at 902.017 kb on + strand, within MMP_RS04720
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 900,074 + -1.7 900,265 - -2.0 900,298 - -2.2 900,299 + -0.7 900,299 + -1.4 900,299 + +0.1 900,299 + -0.7 900,299 + -2.0 900,299 + -1.6 900,323 + -0.9 900,464 - -0.6 900,465 + -1.3 900,501 - -2.0 900,913 - -1.4 900,914 + -1.6 900,914 + -3.8 900,914 + -0.7 900,916 + -1.9 900,939 - -1.2 900,940 + -1.0 901,094 - MMP_RS04710 0.41 -1.1 901,169 - MMP_RS04710 0.60 -0.5 901,272 + MMP_RS04710 0.86 -0.3 901,286 - -0.4 901,304 - -1.5 901,307 - -0.3 901,308 + +0.5 901,308 + -0.8 901,308 + +1.7 901,308 + -0.7 901,341 - -1.4 901,360 - -1.3 901,362 - -0.5 901,362 - -1.7 901,362 - -0.6 901,363 + -0.6 901,363 + -0.8 901,363 + -0.5 901,365 + -0.2 901,365 + +0.4 901,365 + -2.0 901,383 - -2.0 901,393 - -0.4 901,402 + -0.7 901,403 - +0.1 901,403 - -0.6 901,403 - -0.7 901,404 + -0.3 901,404 + -3.4 901,404 + -0.8 901,407 - -0.7 901,407 - -0.1 901,408 + -1.2 901,423 + -0.9 901,453 - -0.4 901,470 - -0.7 901,470 - -1.8 901,474 - -1.0 901,474 - -0.1 901,475 + -0.3 901,476 - -2.2 901,476 - -1.0 901,477 + -0.8 901,477 + -1.3 901,478 - -0.9 901,478 - +0.1 901,479 + -0.2 901,479 + -0.3 901,481 - -0.5 901,511 + -0.4 901,582 - MMP_RS04715 0.19 +2.0 901,583 + MMP_RS04715 0.19 +0.0 901,583 + MMP_RS04715 0.19 -0.7 901,598 + MMP_RS04715 0.23 -0.2 901,620 - MMP_RS04715 0.29 +0.3 901,626 - MMP_RS04715 0.30 -0.1 901,627 + MMP_RS04715 0.30 -0.4 901,636 - MMP_RS04715 0.33 +0.2 901,648 - MMP_RS04715 0.36 +0.5 901,649 + MMP_RS04715 0.36 +0.1 901,650 - MMP_RS04715 0.36 +0.3 901,702 + MMP_RS04715 0.50 -0.3 901,710 - MMP_RS04715 0.52 +0.1 901,710 - MMP_RS04715 0.52 -0.2 901,729 - MMP_RS04715 0.57 -0.0 901,729 - MMP_RS04715 0.57 +0.9 901,740 - MMP_RS04715 0.60 +0.5 901,750 + MMP_RS04715 0.63 +0.2 901,931 + -0.4 902,011 + MMP_RS04720 0.22 +1.5 902,012 - MMP_RS04720 0.22 +0.1 902,016 - MMP_RS04720 0.23 +1.1 902,017 + MMP_RS04720 0.23 -0.6 902,017 + MMP_RS04720 0.23 -0.3
Or see this region's nucleotide sequence