Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02155 and MMP_RS02160 are separated by 105 nucleotides MMP_RS02160 and MMP_RS02165 are separated by 27 nucleotides MMP_RS02165 and MMP_RS02170 are separated by 14 nucleotides MMP_RS02170 and MMP_RS02175 are separated by 158 nucleotides
MMP_RS02155: MMP_RS02155 - 2-phospho-L-lactate transferase, at 403,481 to 404,410
_RS02155
MMP_RS02160: MMP_RS02160 - GTP cyclohydrolase IIa, at 404,516 to 405,316
_RS02160
MMP_RS02165: MMP_RS02165 - tRNA-dihydrouridine synthase, at 405,344 to 406,114
_RS02165
MMP_RS02170: MMP_RS02170 - CBS domain-containing protein, at 406,129 to 406,542
_RS02170
MMP_RS02175: MMP_RS02175 - DUF1611 domain-containing protein, at 406,701 to 407,621
_RS02175
Position (kb) 405
406
407 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 404.452 kb on - strand at 404.455 kb on + strand at 404.500 kb on + strand at 404.511 kb on - strand at 404.619 kb on + strand at 405.112 kb on - strand at 405.289 kb on + strand at 405.294 kb on + strand at 405.299 kb on - strand at 405.299 kb on - strand at 405.354 kb on - strand at 405.434 kb on - strand, within MMP_RS02165 at 405.657 kb on - strand, within MMP_RS02165 at 405.658 kb on + strand, within MMP_RS02165 at 405.682 kb on - strand, within MMP_RS02165 at 405.842 kb on + strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.911 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.949 kb on + strand, within MMP_RS02165 at 406.078 kb on + strand at 406.087 kb on - strand at 406.087 kb on - strand at 406.088 kb on + strand at 406.117 kb on + strand at 406.117 kb on + strand at 406.117 kb on + strand at 406.121 kb on - strand at 406.130 kb on - strand at 406.135 kb on - strand at 406.200 kb on - strand, within MMP_RS02170 at 406.220 kb on + strand, within MMP_RS02170 at 406.354 kb on - strand, within MMP_RS02170 at 406.354 kb on - strand, within MMP_RS02170 at 406.509 kb on - strand at 406.562 kb on - strand at 406.566 kb on + strand at 406.592 kb on + strand at 406.602 kb on + strand at 406.626 kb on - strand at 406.770 kb on + strand at 406.772 kb on + strand at 406.772 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 404,452 - -2.7 404,455 + -2.8 404,500 + -1.9 404,511 - -3.2 404,619 + -2.3 405,112 - -0.7 405,289 + -2.0 405,294 + -0.0 405,299 - -0.8 405,299 - -0.4 405,354 - -1.0 405,434 - MMP_RS02165 0.12 -1.6 405,657 - MMP_RS02165 0.41 -1.2 405,658 + MMP_RS02165 0.41 -2.3 405,682 - MMP_RS02165 0.44 -1.1 405,842 + MMP_RS02165 0.65 -0.0 405,910 - MMP_RS02165 0.73 -0.5 405,910 - MMP_RS02165 0.73 -1.7 405,910 - MMP_RS02165 0.73 -1.3 405,911 + MMP_RS02165 0.74 -1.6 405,913 + MMP_RS02165 0.74 -0.4 405,913 + MMP_RS02165 0.74 -2.0 405,913 + MMP_RS02165 0.74 -1.0 405,913 + MMP_RS02165 0.74 -0.5 405,913 + MMP_RS02165 0.74 +0.2 405,913 + MMP_RS02165 0.74 -1.2 405,949 + MMP_RS02165 0.78 -0.9 406,078 + -1.1 406,087 - -0.6 406,087 - -1.2 406,088 + -1.0 406,117 + -0.2 406,117 + +1.6 406,117 + -0.4 406,121 - -0.4 406,130 - -0.2 406,135 - -1.3 406,200 - MMP_RS02170 0.17 -0.2 406,220 + MMP_RS02170 0.22 -0.1 406,354 - MMP_RS02170 0.54 -0.6 406,354 - MMP_RS02170 0.54 +0.2 406,509 - -0.3 406,562 - -0.4 406,566 + -0.1 406,592 + -0.3 406,602 + -0.4 406,626 - -0.1 406,770 + -0.2 406,772 + -0.5 406,772 + -0.8
Or see this region's nucleotide sequence