Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01825 and MMP_RS01830 are separated by 105 nucleotides MMP_RS01830 and MMP_RS01835 are separated by 110 nucleotides MMP_RS01835 and MMP_RS01840 are separated by 0 nucleotides MMP_RS01840 and MMP_RS01845 are separated by 52 nucleotides
MMP_RS01825: MMP_RS01825 - acetyl-CoA carboxylase biotin carboxylase subunit, at 337,634 to 339,139
_RS01825
MMP_RS01830: MMP_RS01830 - tRNA-Val, at 339,245 to 339,321
_RS01830
MMP_RS01835: MMP_RS01835 - hypothetical protein, at 339,432 to 339,866
_RS01835
MMP_RS01840: MMP_RS01840 - TIGR00289 family protein, at 339,867 to 340,538
_RS01840
MMP_RS01845: MMP_RS01845 - hypothetical protein, at 340,591 to 341,472
_RS01845
Position (kb)
339
340
341 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 338.887 kb on + strand at 338.949 kb on + strand at 338.959 kb on - strand at 339.094 kb on + strand at 339.122 kb on + strand at 339.152 kb on + strand at 339.155 kb on - strand at 339.160 kb on - strand at 339.160 kb on - strand at 339.166 kb on - strand at 339.166 kb on - strand at 339.206 kb on + strand at 339.215 kb on + strand at 339.224 kb on + strand at 339.234 kb on + strand at 339.240 kb on - strand at 339.257 kb on - strand, within MMP_RS01830 at 339.258 kb on + strand, within MMP_RS01830 at 339.451 kb on + strand at 339.530 kb on - strand, within MMP_RS01835 at 339.535 kb on - strand, within MMP_RS01835 at 339.558 kb on - strand, within MMP_RS01835 at 339.635 kb on - strand, within MMP_RS01835 at 339.635 kb on - strand, within MMP_RS01835 at 339.636 kb on + strand, within MMP_RS01835 at 339.736 kb on + strand, within MMP_RS01835 at 339.737 kb on - strand, within MMP_RS01835 at 339.763 kb on - strand, within MMP_RS01835 at 339.765 kb on - strand, within MMP_RS01835 at 339.812 kb on + strand, within MMP_RS01835 at 339.865 kb on + strand at 339.899 kb on - strand at 339.942 kb on - strand, within MMP_RS01840 at 339.951 kb on - strand, within MMP_RS01840 at 340.266 kb on - strand, within MMP_RS01840 at 340.267 kb on + strand, within MMP_RS01840 at 340.338 kb on + strand, within MMP_RS01840 at 340.340 kb on - strand, within MMP_RS01840 at 340.345 kb on + strand, within MMP_RS01840 at 340.417 kb on - strand, within MMP_RS01840 at 340.453 kb on - strand, within MMP_RS01840 at 340.453 kb on - strand, within MMP_RS01840 at 340.453 kb on - strand, within MMP_RS01840 at 340.454 kb on + strand, within MMP_RS01840 at 340.454 kb on + strand, within MMP_RS01840 at 340.708 kb on - strand, within MMP_RS01845 at 340.711 kb on + strand, within MMP_RS01845 at 340.714 kb on + strand, within MMP_RS01845 at 340.726 kb on - strand, within MMP_RS01845 at 340.763 kb on - strand, within MMP_RS01845 at 340.763 kb on - strand, within MMP_RS01845 at 340.769 kb on - strand, within MMP_RS01845 at 340.769 kb on - strand, within MMP_RS01845 at 340.774 kb on + strand, within MMP_RS01845 at 340.781 kb on - strand, within MMP_RS01845 at 340.790 kb on - strand, within MMP_RS01845 at 340.791 kb on + strand, within MMP_RS01845 at 340.848 kb on - strand, within MMP_RS01845 at 340.888 kb on - strand, within MMP_RS01845 at 340.888 kb on - strand, within MMP_RS01845 at 340.888 kb on - strand, within MMP_RS01845 at 340.889 kb on + strand, within MMP_RS01845 at 340.918 kb on - strand, within MMP_RS01845 at 341.012 kb on - strand, within MMP_RS01845 at 341.044 kb on + strand, within MMP_RS01845 at 341.070 kb on - strand, within MMP_RS01845 at 341.132 kb on + strand, within MMP_RS01845 at 341.133 kb on - strand, within MMP_RS01845 at 341.139 kb on - strand, within MMP_RS01845 at 341.140 kb on + strand, within MMP_RS01845 at 341.140 kb on + strand, within MMP_RS01845 at 341.140 kb on + strand, within MMP_RS01845 at 341.142 kb on + strand, within MMP_RS01845 at 341.181 kb on + strand, within MMP_RS01845 at 341.218 kb on - strand, within MMP_RS01845 at 341.219 kb on + strand, within MMP_RS01845 at 341.221 kb on + strand, within MMP_RS01845 at 341.249 kb on + strand, within MMP_RS01845 at 341.330 kb on - strand, within MMP_RS01845 at 341.330 kb on - strand, within MMP_RS01845 at 341.472 kb on - strand at 341.472 kb on - strand at 341.475 kb on - strand at 341.475 kb on - strand at 341.476 kb on + strand at 341.476 kb on + strand at 341.476 kb on + strand at 341.476 kb on + strand at 341.501 kb on - strand at 341.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 338,887 + -1.9 338,949 + -0.4 338,959 - -2.8 339,094 + -0.7 339,122 + -0.1 339,152 + +0.1 339,155 - +0.0 339,160 - -0.3 339,160 - +0.2 339,166 - -0.4 339,166 - +0.8 339,206 + -0.4 339,215 + +0.6 339,224 + -0.1 339,234 + -0.3 339,240 - -0.6 339,257 - MMP_RS01830 0.16 -0.8 339,258 + MMP_RS01830 0.17 -0.9 339,451 + +0.4 339,530 - MMP_RS01835 0.23 +0.0 339,535 - MMP_RS01835 0.24 +0.4 339,558 - MMP_RS01835 0.29 +0.5 339,635 - MMP_RS01835 0.47 -0.3 339,635 - MMP_RS01835 0.47 -0.5 339,636 + MMP_RS01835 0.47 +0.0 339,736 + MMP_RS01835 0.70 +0.3 339,737 - MMP_RS01835 0.70 -0.1 339,763 - MMP_RS01835 0.76 -0.0 339,765 - MMP_RS01835 0.77 -0.1 339,812 + MMP_RS01835 0.87 -0.2 339,865 + +0.4 339,899 - -0.7 339,942 - MMP_RS01840 0.11 -0.3 339,951 - MMP_RS01840 0.12 -1.2 340,266 - MMP_RS01840 0.59 -0.5 340,267 + MMP_RS01840 0.60 -0.6 340,338 + MMP_RS01840 0.70 -0.4 340,340 - MMP_RS01840 0.70 -0.1 340,345 + MMP_RS01840 0.71 -1.1 340,417 - MMP_RS01840 0.82 -0.4 340,453 - MMP_RS01840 0.87 -0.3 340,453 - MMP_RS01840 0.87 -0.5 340,453 - MMP_RS01840 0.87 +0.2 340,454 + MMP_RS01840 0.87 -0.0 340,454 + MMP_RS01840 0.87 -0.6 340,708 - MMP_RS01845 0.13 +0.2 340,711 + MMP_RS01845 0.14 +0.3 340,714 + MMP_RS01845 0.14 -0.5 340,726 - MMP_RS01845 0.15 -0.3 340,763 - MMP_RS01845 0.20 -0.1 340,763 - MMP_RS01845 0.20 -0.2 340,769 - MMP_RS01845 0.20 -0.2 340,769 - MMP_RS01845 0.20 +0.1 340,774 + MMP_RS01845 0.21 -0.1 340,781 - MMP_RS01845 0.22 +0.1 340,790 - MMP_RS01845 0.23 +0.1 340,791 + MMP_RS01845 0.23 +0.3 340,848 - MMP_RS01845 0.29 +0.0 340,888 - MMP_RS01845 0.34 +0.5 340,888 - MMP_RS01845 0.34 +0.2 340,888 - MMP_RS01845 0.34 -0.1 340,889 + MMP_RS01845 0.34 +0.0 340,918 - MMP_RS01845 0.37 -0.0 341,012 - MMP_RS01845 0.48 -0.1 341,044 + MMP_RS01845 0.51 -0.3 341,070 - MMP_RS01845 0.54 -0.1 341,132 + MMP_RS01845 0.61 +0.4 341,133 - MMP_RS01845 0.61 -0.2 341,139 - MMP_RS01845 0.62 +0.0 341,140 + MMP_RS01845 0.62 +1.2 341,140 + MMP_RS01845 0.62 +0.1 341,140 + MMP_RS01845 0.62 +0.0 341,142 + MMP_RS01845 0.62 -0.6 341,181 + MMP_RS01845 0.67 +0.5 341,218 - MMP_RS01845 0.71 +0.1 341,219 + MMP_RS01845 0.71 -0.1 341,221 + MMP_RS01845 0.71 +0.2 341,249 + MMP_RS01845 0.75 -0.0 341,330 - MMP_RS01845 0.84 +0.4 341,330 - MMP_RS01845 0.84 -0.1 341,472 - -0.7 341,472 - +0.4 341,475 - -0.1 341,475 - -0.0 341,476 + +0.7 341,476 + +0.4 341,476 + -0.0 341,476 + -0.4 341,501 - +0.3 341,501 - +0.3
Or see this region's nucleotide sequence