Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01710 and MMP_RS01715 are separated by 132 nucleotides MMP_RS01715 and MMP_RS01720 are separated by 0 nucleotides MMP_RS01720 and MMP_RS01725 are separated by 9 nucleotides
MMP_RS01710: MMP_RS01710 - shikimate kinase, at 316,643 to 317,494
_RS01710
MMP_RS01715: MMP_RS01715 - DUF447 family protein, at 317,627 to 318,172
_RS01715
MMP_RS01720: MMP_RS01720 - replication factor C large subunit, at 318,173 to 319,633
_RS01720
MMP_RS01725: MMP_RS01725 - class I SAM-dependent methyltransferase family protein, at 319,643 to 320,671
_RS01725
Position (kb) 318
319
320 Strain fitness (log2 ratio) -2
-1
0
1 at 317.543 kb on + strand at 317.549 kb on - strand at 317.558 kb on + strand at 317.559 kb on - strand at 317.570 kb on - strand at 317.570 kb on - strand at 317.573 kb on - strand at 317.573 kb on - strand at 317.576 kb on - strand at 317.578 kb on - strand at 317.579 kb on + strand at 317.581 kb on - strand at 317.581 kb on - strand at 317.587 kb on + strand at 317.587 kb on + strand at 317.614 kb on + strand at 317.626 kb on - strand at 317.627 kb on + strand at 317.718 kb on + strand at 317.718 kb on + strand at 317.762 kb on - strand at 317.770 kb on + strand at 317.773 kb on - strand at 317.774 kb on + strand at 317.786 kb on - strand at 317.865 kb on - strand at 318.064 kb on - strand at 318.065 kb on + strand at 318.096 kb on - strand at 318.100 kb on - strand at 318.268 kb on - strand at 318.525 kb on - strand, within MMP_RS01720 at 318.573 kb on - strand, within MMP_RS01720 at 319.396 kb on + strand, within MMP_RS01720 at 319.656 kb on + strand at 319.684 kb on + strand at 319.786 kb on - strand, within MMP_RS01725 at 319.831 kb on - strand, within MMP_RS01725 at 319.871 kb on + strand, within MMP_RS01725 at 319.985 kb on - strand, within MMP_RS01725 at 319.999 kb on + strand, within MMP_RS01725 at 320.173 kb on + strand, within MMP_RS01725 at 320.432 kb on + strand, within MMP_RS01725 at 320.584 kb on - strand at 320.594 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 317,543 + -1.6 317,549 - -1.0 317,558 + -1.7 317,559 - -0.3 317,570 - -0.5 317,570 - +0.4 317,573 - -0.0 317,573 - -0.2 317,576 - -0.5 317,578 - -0.5 317,579 + +0.0 317,581 - -1.1 317,581 - -0.8 317,587 + -0.1 317,587 + -0.4 317,614 + -1.9 317,626 - -0.5 317,627 + -1.0 317,718 + -1.0 317,718 + +0.6 317,762 - -0.7 317,770 + +0.0 317,773 - -1.0 317,774 + -1.8 317,786 - -1.8 317,865 - -1.6 318,064 - -1.7 318,065 + -1.4 318,096 - -2.1 318,100 - -0.8 318,268 - -1.7 318,525 - MMP_RS01720 0.24 -0.6 318,573 - MMP_RS01720 0.27 -0.8 319,396 + MMP_RS01720 0.84 -0.1 319,656 + +0.3 319,684 + -0.8 319,786 - MMP_RS01725 0.14 -0.0 319,831 - MMP_RS01725 0.18 +0.0 319,871 + MMP_RS01725 0.22 -0.1 319,985 - MMP_RS01725 0.33 +0.7 319,999 + MMP_RS01725 0.35 -1.4 320,173 + MMP_RS01725 0.52 +0.3 320,432 + MMP_RS01725 0.77 -0.1 320,584 - -0.4 320,594 + +1.6
Or see this region's nucleotide sequence