Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01375 and MMP_RS01380 are separated by 20 nucleotides MMP_RS01380 and MMP_RS01385 are separated by 35 nucleotides MMP_RS01385 and MMP_RS01390 are separated by 11 nucleotides MMP_RS01390 and MMP_RS01395 are separated by 131 nucleotides
MMP_RS01375: MMP_RS01375 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 255,837 to 257,360
_RS01375
MMP_RS01380: MMP_RS01380 - PHP domain-containing protein, at 257,381 to 258,280
_RS01380
MMP_RS01385: MMP_RS01385 - imidazole glycerol phosphate synthase subunit HisH, at 258,316 to 258,927
_RS01385
MMP_RS01390: MMP_RS01390 - TATA-box-binding protein, at 258,939 to 259,484
_RS01390
MMP_RS01395: MMP_RS01395 - 50S ribosomal protein P1, at 259,616 to 259,915
_RS01395
Position (kb) 258
259 Strain fitness (log2 ratio) -2
-1
0
1 at 257.354 kb on + strand at 257.354 kb on + strand at 257.358 kb on - strand at 257.395 kb on + strand at 257.415 kb on - strand at 257.444 kb on - strand at 257.619 kb on - strand, within MMP_RS01380 at 257.620 kb on + strand, within MMP_RS01380 at 257.839 kb on + strand, within MMP_RS01380 at 257.842 kb on - strand, within MMP_RS01380 at 258.008 kb on - strand, within MMP_RS01380 at 258.066 kb on + strand, within MMP_RS01380 at 258.121 kb on + strand, within MMP_RS01380 at 258.126 kb on + strand, within MMP_RS01380 at 258.176 kb on - strand, within MMP_RS01380 at 258.220 kb on - strand at 258.223 kb on + strand at 258.309 kb on - strand at 258.314 kb on + strand at 258.314 kb on + strand at 258.865 kb on - strand, within MMP_RS01385 at 258.908 kb on - strand at 258.943 kb on - strand at 258.974 kb on - strand at 258.993 kb on - strand at 258.997 kb on - strand at 258.998 kb on + strand at 258.998 kb on + strand at 259.147 kb on + strand at 259.525 kb on + strand at 259.903 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 257,354 + -0.6 257,354 + -0.4 257,358 - -1.3 257,395 + -2.4 257,415 - -1.3 257,444 - -0.9 257,619 - MMP_RS01380 0.26 -0.3 257,620 + MMP_RS01380 0.27 -0.7 257,839 + MMP_RS01380 0.51 -0.3 257,842 - MMP_RS01380 0.51 -0.3 258,008 - MMP_RS01380 0.70 -0.7 258,066 + MMP_RS01380 0.76 -0.1 258,121 + MMP_RS01380 0.82 -0.9 258,126 + MMP_RS01380 0.83 -0.9 258,176 - MMP_RS01380 0.88 -1.1 258,220 - -0.1 258,223 + -1.0 258,309 - +0.6 258,314 + -0.6 258,314 + -0.0 258,865 - MMP_RS01385 0.90 -1.4 258,908 - -0.8 258,943 - +0.3 258,974 - -1.3 258,993 - -0.7 258,997 - -0.9 258,998 + -1.1 258,998 + -1.5 259,147 + -0.8 259,525 + -1.1 259,903 + -2.3
Or see this region's nucleotide sequence