Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01375 and MMP_RS01380 are separated by 20 nucleotides MMP_RS01380 and MMP_RS01385 are separated by 35 nucleotides MMP_RS01385 and MMP_RS01390 are separated by 11 nucleotides
MMP_RS01375: MMP_RS01375 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 255,837 to 257,360
_RS01375
MMP_RS01380: MMP_RS01380 - PHP domain-containing protein, at 257,381 to 258,280
_RS01380
MMP_RS01385: MMP_RS01385 - imidazole glycerol phosphate synthase subunit HisH, at 258,316 to 258,927
_RS01385
MMP_RS01390: MMP_RS01390 - TATA-box-binding protein, at 258,939 to 259,484
_RS01390
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1 at 256.402 kb on - strand, within MMP_RS01375 at 256.418 kb on - strand, within MMP_RS01375 at 256.419 kb on + strand, within MMP_RS01375 at 256.450 kb on - strand, within MMP_RS01375 at 256.451 kb on + strand, within MMP_RS01375 at 256.455 kb on + strand, within MMP_RS01375 at 256.455 kb on + strand, within MMP_RS01375 at 256.490 kb on - strand, within MMP_RS01375 at 256.492 kb on - strand, within MMP_RS01375 at 256.493 kb on + strand, within MMP_RS01375 at 256.561 kb on + strand, within MMP_RS01375 at 256.796 kb on + strand, within MMP_RS01375 at 256.807 kb on + strand, within MMP_RS01375 at 256.809 kb on + strand, within MMP_RS01375 at 256.809 kb on + strand, within MMP_RS01375 at 256.825 kb on - strand, within MMP_RS01375 at 256.834 kb on - strand, within MMP_RS01375 at 256.874 kb on - strand, within MMP_RS01375 at 256.888 kb on - strand, within MMP_RS01375 at 256.912 kb on - strand, within MMP_RS01375 at 256.921 kb on + strand, within MMP_RS01375 at 256.949 kb on - strand, within MMP_RS01375 at 256.980 kb on - strand, within MMP_RS01375 at 256.983 kb on + strand, within MMP_RS01375 at 256.983 kb on + strand, within MMP_RS01375 at 257.009 kb on - strand, within MMP_RS01375 at 257.010 kb on + strand, within MMP_RS01375 at 257.012 kb on + strand, within MMP_RS01375 at 257.015 kb on - strand, within MMP_RS01375 at 257.040 kb on - strand, within MMP_RS01375 at 257.052 kb on - strand, within MMP_RS01375 at 257.083 kb on - strand, within MMP_RS01375 at 257.090 kb on + strand, within MMP_RS01375 at 257.108 kb on + strand, within MMP_RS01375 at 257.108 kb on + strand, within MMP_RS01375 at 257.110 kb on - strand, within MMP_RS01375 at 257.121 kb on + strand, within MMP_RS01375 at 257.181 kb on + strand, within MMP_RS01375 at 257.182 kb on - strand, within MMP_RS01375 at 257.183 kb on + strand, within MMP_RS01375 at 257.183 kb on + strand, within MMP_RS01375 at 257.209 kb on + strand at 257.251 kb on + strand at 257.354 kb on + strand at 257.354 kb on + strand at 257.358 kb on - strand at 257.395 kb on + strand at 257.415 kb on - strand at 257.444 kb on - strand at 257.619 kb on - strand, within MMP_RS01380 at 257.620 kb on + strand, within MMP_RS01380 at 257.839 kb on + strand, within MMP_RS01380 at 257.842 kb on - strand, within MMP_RS01380 at 258.008 kb on - strand, within MMP_RS01380 at 258.066 kb on + strand, within MMP_RS01380 at 258.121 kb on + strand, within MMP_RS01380 at 258.126 kb on + strand, within MMP_RS01380 at 258.176 kb on - strand, within MMP_RS01380 at 258.220 kb on - strand at 258.223 kb on + strand at 258.309 kb on - strand at 258.314 kb on + strand at 258.314 kb on + strand at 258.865 kb on - strand, within MMP_RS01385 at 258.908 kb on - strand at 258.943 kb on - strand at 258.974 kb on - strand at 258.993 kb on - strand at 258.997 kb on - strand at 258.998 kb on + strand at 258.998 kb on + strand at 259.147 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 256,402 - MMP_RS01375 0.37 +0.1 256,418 - MMP_RS01375 0.38 +0.0 256,419 + MMP_RS01375 0.38 -0.5 256,450 - MMP_RS01375 0.40 -0.2 256,451 + MMP_RS01375 0.40 +0.5 256,455 + MMP_RS01375 0.41 -0.5 256,455 + MMP_RS01375 0.41 -0.2 256,490 - MMP_RS01375 0.43 -0.3 256,492 - MMP_RS01375 0.43 +0.2 256,493 + MMP_RS01375 0.43 -0.2 256,561 + MMP_RS01375 0.48 -0.1 256,796 + MMP_RS01375 0.63 -1.3 256,807 + MMP_RS01375 0.64 -0.2 256,809 + MMP_RS01375 0.64 -0.2 256,809 + MMP_RS01375 0.64 -0.0 256,825 - MMP_RS01375 0.65 -0.2 256,834 - MMP_RS01375 0.65 -0.6 256,874 - MMP_RS01375 0.68 +1.0 256,888 - MMP_RS01375 0.69 +0.2 256,912 - MMP_RS01375 0.71 -0.1 256,921 + MMP_RS01375 0.71 +0.1 256,949 - MMP_RS01375 0.73 -0.2 256,980 - MMP_RS01375 0.75 -0.1 256,983 + MMP_RS01375 0.75 -0.1 256,983 + MMP_RS01375 0.75 +0.1 257,009 - MMP_RS01375 0.77 +0.6 257,010 + MMP_RS01375 0.77 -0.4 257,012 + MMP_RS01375 0.77 -0.5 257,015 - MMP_RS01375 0.77 +0.1 257,040 - MMP_RS01375 0.79 +0.4 257,052 - MMP_RS01375 0.80 -0.8 257,083 - MMP_RS01375 0.82 +0.3 257,090 + MMP_RS01375 0.82 -0.1 257,108 + MMP_RS01375 0.83 -0.1 257,108 + MMP_RS01375 0.83 -0.0 257,110 - MMP_RS01375 0.84 -0.0 257,121 + MMP_RS01375 0.84 -0.6 257,181 + MMP_RS01375 0.88 -0.2 257,182 - MMP_RS01375 0.88 -0.6 257,183 + MMP_RS01375 0.88 -0.5 257,183 + MMP_RS01375 0.88 -0.4 257,209 + -0.2 257,251 + +0.1 257,354 + -0.6 257,354 + -0.4 257,358 - -1.3 257,395 + -2.4 257,415 - -1.3 257,444 - -0.9 257,619 - MMP_RS01380 0.26 -0.3 257,620 + MMP_RS01380 0.27 -0.7 257,839 + MMP_RS01380 0.51 -0.3 257,842 - MMP_RS01380 0.51 -0.3 258,008 - MMP_RS01380 0.70 -0.7 258,066 + MMP_RS01380 0.76 -0.1 258,121 + MMP_RS01380 0.82 -0.9 258,126 + MMP_RS01380 0.83 -0.9 258,176 - MMP_RS01380 0.88 -1.1 258,220 - -0.1 258,223 + -1.0 258,309 - +0.6 258,314 + -0.6 258,314 + -0.0 258,865 - MMP_RS01385 0.90 -1.4 258,908 - -0.8 258,943 - +0.3 258,974 - -1.3 258,993 - -0.7 258,997 - -0.9 258,998 + -1.1 258,998 + -1.5 259,147 + -0.8
Or see this region's nucleotide sequence