Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01170 and MMP_RS01175 are separated by 11 nucleotides MMP_RS01175 and MMP_RS01180 are separated by 11 nucleotides MMP_RS01180 and MMP_RS01185 are separated by 115 nucleotides
MMP_RS01170: MMP_RS01170 - glycine--tRNA ligase, at 218,916 to 220,640
_RS01170
MMP_RS01175: MMP_RS01175 - triphosphoribosyl-dephospho-CoA synthase, at 220,652 to 221,533
_RS01175
MMP_RS01180: MMP_RS01180 - XTP/dITP diphosphatase, at 221,545 to 222,096
_RS01180
MMP_RS01185: MMP_RS01185 - hypothetical protein, at 222,212 to 222,622
_RS01185
Position (kb) 220
221
222 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 219.809 kb on + strand at 219.924 kb on - strand at 220.486 kb on - strand at 220.895 kb on - strand, within MMP_RS01175 at 220.948 kb on + strand, within MMP_RS01175 at 220.951 kb on + strand, within MMP_RS01175 at 220.977 kb on - strand, within MMP_RS01175 at 221.236 kb on - strand, within MMP_RS01175 at 221.241 kb on + strand, within MMP_RS01175 at 221.568 kb on - strand at 221.759 kb on - strand, within MMP_RS01180 at 221.915 kb on + strand, within MMP_RS01180 at 222.063 kb on - strand at 222.086 kb on - strand at 222.097 kb on + strand at 222.134 kb on - strand at 222.135 kb on + strand at 222.142 kb on + strand at 222.158 kb on - strand at 222.158 kb on - strand at 222.158 kb on - strand at 222.159 kb on + strand at 222.171 kb on + strand at 222.186 kb on + strand at 222.188 kb on - strand at 222.444 kb on - strand, within MMP_RS01185 at 222.444 kb on - strand, within MMP_RS01185 at 222.447 kb on + strand, within MMP_RS01185 at 222.449 kb on + strand, within MMP_RS01185 at 222.475 kb on - strand, within MMP_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 219,809 + -1.0 219,924 - -1.3 220,486 - -1.5 220,895 - MMP_RS01175 0.28 -2.3 220,948 + MMP_RS01175 0.34 -3.0 220,951 + MMP_RS01175 0.34 -2.5 220,977 - MMP_RS01175 0.37 -4.0 221,236 - MMP_RS01175 0.66 -3.6 221,241 + MMP_RS01175 0.67 -2.6 221,568 - +0.5 221,759 - MMP_RS01180 0.39 +0.4 221,915 + MMP_RS01180 0.67 -1.2 222,063 - +0.1 222,086 - -0.0 222,097 + -0.2 222,134 - +0.2 222,135 + -0.7 222,142 + -0.8 222,158 - -0.1 222,158 - +0.1 222,158 - -0.3 222,159 + -1.2 222,171 + -0.2 222,186 + -0.1 222,188 - +0.1 222,444 - MMP_RS01185 0.56 +0.1 222,444 - MMP_RS01185 0.56 +0.4 222,447 + MMP_RS01185 0.57 +0.2 222,449 + MMP_RS01185 0.58 -0.0 222,475 - MMP_RS01185 0.64 +0.8
Or see this region's nucleotide sequence