Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00715 and MMP_RS00720 are separated by 27 nucleotides MMP_RS00720 and MMP_RS00725 are separated by 40 nucleotides MMP_RS00725 and MMP_RS00730 are separated by 28 nucleotides
MMP_RS00715: MMP_RS00715 - formate-dependent phosphoribosylglycinamide formyltransferase, at 129,070 to 130,236
_RS00715
MMP_RS00720: MMP_RS00720 - metal-dependent hydrolase, at 130,264 to 130,938
_RS00720
MMP_RS00725: MMP_RS00725 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC, at 130,979 to 132,511
_RS00725
MMP_RS00730: MMP_RS00730 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 132,540 to 133,565
_RS00730
Position (kb) 130
131
132
133 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 130.010 kb on - strand at 130.115 kb on - strand at 130.146 kb on - strand at 130.284 kb on + strand at 130.292 kb on + strand at 130.477 kb on + strand, within MMP_RS00720 at 130.534 kb on + strand, within MMP_RS00720 at 130.650 kb on - strand, within MMP_RS00720 at 130.683 kb on - strand, within MMP_RS00720 at 130.684 kb on + strand, within MMP_RS00720 at 130.685 kb on - strand, within MMP_RS00720 at 130.748 kb on + strand, within MMP_RS00720 at 130.751 kb on - strand, within MMP_RS00720 at 130.752 kb on + strand, within MMP_RS00720 at 130.773 kb on - strand, within MMP_RS00720 at 130.790 kb on - strand, within MMP_RS00720 at 130.791 kb on + strand, within MMP_RS00720 at 130.807 kb on - strand, within MMP_RS00720 at 130.808 kb on + strand, within MMP_RS00720 at 130.819 kb on - strand, within MMP_RS00720 at 130.831 kb on - strand, within MMP_RS00720 at 130.887 kb on - strand at 130.907 kb on + strand at 130.918 kb on + strand at 130.986 kb on + strand at 131.041 kb on - strand at 131.092 kb on - strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.123 kb on - strand at 131.173 kb on + strand, within MMP_RS00725 at 131.173 kb on + strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.175 kb on + strand, within MMP_RS00725 at 131.612 kb on + strand, within MMP_RS00725 at 131.657 kb on + strand, within MMP_RS00725 at 131.738 kb on - strand, within MMP_RS00725 at 131.786 kb on - strand, within MMP_RS00725 at 132.136 kb on - strand, within MMP_RS00725 at 132.392 kb on - strand at 132.439 kb on - strand at 132.582 kb on - strand at 132.583 kb on + strand at 132.583 kb on + strand at 132.583 kb on + strand at 132.583 kb on + strand at 132.899 kb on - strand, within MMP_RS00730 at 132.942 kb on - strand, within MMP_RS00730 at 133.070 kb on - strand, within MMP_RS00730 at 133.104 kb on - strand, within MMP_RS00730 at 133.174 kb on + strand, within MMP_RS00730 at 133.288 kb on + strand, within MMP_RS00730 at 133.362 kb on - strand, within MMP_RS00730 at 133.441 kb on - strand, within MMP_RS00730 at 133.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 130,010 - -0.2 130,115 - -2.0 130,146 - -3.7 130,284 + -0.2 130,292 + -0.2 130,477 + MMP_RS00720 0.32 -0.7 130,534 + MMP_RS00720 0.40 -0.5 130,650 - MMP_RS00720 0.57 +0.3 130,683 - MMP_RS00720 0.62 -0.0 130,684 + MMP_RS00720 0.62 -0.9 130,685 - MMP_RS00720 0.62 -0.7 130,748 + MMP_RS00720 0.72 -0.1 130,751 - MMP_RS00720 0.72 -0.2 130,752 + MMP_RS00720 0.72 -0.5 130,773 - MMP_RS00720 0.75 -0.3 130,790 - MMP_RS00720 0.78 +0.2 130,791 + MMP_RS00720 0.78 +0.0 130,807 - MMP_RS00720 0.80 -0.8 130,808 + MMP_RS00720 0.81 -0.9 130,819 - MMP_RS00720 0.82 -0.6 130,831 - MMP_RS00720 0.84 -0.7 130,887 - -0.8 130,907 + -0.4 130,918 + -0.7 130,986 + -0.2 131,041 - -1.4 131,092 - -0.5 131,101 + -0.9 131,101 + -0.9 131,101 + -1.1 131,123 - -0.9 131,173 + MMP_RS00725 0.13 -1.2 131,173 + MMP_RS00725 0.13 -0.9 131,174 - MMP_RS00725 0.13 -0.4 131,174 - MMP_RS00725 0.13 +0.6 131,175 + MMP_RS00725 0.13 -0.4 131,612 + MMP_RS00725 0.41 -0.2 131,657 + MMP_RS00725 0.44 -0.6 131,738 - MMP_RS00725 0.50 -1.2 131,786 - MMP_RS00725 0.53 -0.5 132,136 - MMP_RS00725 0.75 -1.0 132,392 - -0.5 132,439 - -1.1 132,582 - -1.4 132,583 + -0.9 132,583 + -1.0 132,583 + -0.9 132,583 + -0.6 132,899 - MMP_RS00730 0.35 -0.6 132,942 - MMP_RS00730 0.39 +0.1 133,070 - MMP_RS00730 0.52 -0.8 133,104 - MMP_RS00730 0.55 -0.5 133,174 + MMP_RS00730 0.62 -1.2 133,288 + MMP_RS00730 0.73 -0.7 133,362 - MMP_RS00730 0.80 -0.6 133,441 - MMP_RS00730 0.88 -1.3 133,490 - -0.4
Or see this region's nucleotide sequence