Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00680 and MMP_RS00685 are separated by 23 nucleotides MMP_RS00685 and MMP_RS00690 are separated by 46 nucleotides MMP_RS00690 and MMP_RS00695 are separated by 12 nucleotides
MMP_RS00680: MMP_RS00680 - N-acetyl-gamma-glutamyl-phosphate reductase, at 124,154 to 125,185
_RS00680
MMP_RS00685: MMP_RS00685 - 2-phospho-L-lactate guanylyltransferase, at 125,209 to 125,877
_RS00685
MMP_RS00690: MMP_RS00690 - MBL fold metallo-hydrolase, at 125,924 to 126,541
_RS00690
MMP_RS00695: MMP_RS00695 - biotin--[acetyl-CoA-carboxylase] ligase, at 126,554 to 127,249
_RS00695
Position (kb)
125
126 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 124.339 kb on - strand at 124.480 kb on - strand at 124.484 kb on - strand at 124.485 kb on + strand at 124.873 kb on + strand at 124.907 kb on + strand at 124.907 kb on + strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.909 kb on + strand at 124.946 kb on - strand at 125.264 kb on + strand at 125.289 kb on + strand, within MMP_RS00685 at 125.289 kb on + strand, within MMP_RS00685 at 125.344 kb on - strand, within MMP_RS00685 at 125.344 kb on - strand, within MMP_RS00685 at 125.351 kb on + strand, within MMP_RS00685 at 125.351 kb on + strand, within MMP_RS00685 at 125.358 kb on - strand, within MMP_RS00685 at 125.785 kb on - strand, within MMP_RS00685 at 125.906 kb on - strand at 125.925 kb on - strand at 125.930 kb on + strand at 125.958 kb on + strand at 125.988 kb on + strand, within MMP_RS00690 at 126.008 kb on + strand, within MMP_RS00690 at 126.009 kb on - strand, within MMP_RS00690 at 126.131 kb on - strand, within MMP_RS00690 at 126.170 kb on - strand, within MMP_RS00690 at 126.263 kb on + strand, within MMP_RS00690 at 126.271 kb on - strand, within MMP_RS00690 at 126.280 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.318 kb on + strand, within MMP_RS00690 at 126.391 kb on - strand, within MMP_RS00690 at 126.391 kb on - strand, within MMP_RS00690 at 126.396 kb on - strand, within MMP_RS00690 at 126.491 kb on - strand at 126.671 kb on - strand, within MMP_RS00695 at 126.821 kb on - strand, within MMP_RS00695
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 124,339 - -1.2 124,480 - -1.7 124,484 - +0.4 124,485 + -0.6 124,873 + -0.6 124,907 + -1.4 124,907 + -2.3 124,908 - -1.6 124,908 - -1.2 124,908 - -1.6 124,909 + -0.7 124,946 - +0.1 125,264 + -0.8 125,289 + MMP_RS00685 0.12 -3.4 125,289 + MMP_RS00685 0.12 -1.4 125,344 - MMP_RS00685 0.20 -0.9 125,344 - MMP_RS00685 0.20 -2.0 125,351 + MMP_RS00685 0.21 -0.8 125,351 + MMP_RS00685 0.21 -1.5 125,358 - MMP_RS00685 0.22 -1.4 125,785 - MMP_RS00685 0.86 -2.7 125,906 - +0.3 125,925 - -0.0 125,930 + +0.2 125,958 + +1.1 125,988 + MMP_RS00690 0.10 -0.5 126,008 + MMP_RS00690 0.14 +0.0 126,009 - MMP_RS00690 0.14 -0.6 126,131 - MMP_RS00690 0.33 -0.1 126,170 - MMP_RS00690 0.40 -0.3 126,263 + MMP_RS00690 0.55 +0.6 126,271 - MMP_RS00690 0.56 +0.1 126,280 - MMP_RS00690 0.58 +0.2 126,305 - MMP_RS00690 0.62 +0.4 126,305 - MMP_RS00690 0.62 +0.1 126,305 - MMP_RS00690 0.62 -0.1 126,318 + MMP_RS00690 0.64 -0.7 126,391 - MMP_RS00690 0.76 +0.4 126,391 - MMP_RS00690 0.76 +0.0 126,396 - MMP_RS00690 0.76 +0.2 126,491 - +0.3 126,671 - MMP_RS00695 0.17 -0.3 126,821 - MMP_RS00695 0.38 -1.8
Or see this region's nucleotide sequence