Experiment: Cephalothin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24170 and AO356_24175 overlap by 4 nucleotides AO356_24175 and AO356_24180 are separated by 89 nucleotides AO356_24180 and AO356_24185 are separated by 335 nucleotides
AO356_24170: AO356_24170 - formate dehydrogenase, at 5,419,772 to 5,422,648
_24170
AO356_24175: AO356_24175 - formate dehydrogenase, at 5,422,645 to 5,422,866
_24175
AO356_24180: AO356_24180 - nematocidal protein, at 5,422,956 to 5,423,486
_24180
AO356_24185: AO356_24185 - isochorismate-pyruvate lyase, at 5,423,822 to 5,424,142
_24185
Position (kb)
5422
5423 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5421.696 kb on - strand, within AO356_24170 at 5421.696 kb on - strand, within AO356_24170 at 5421.696 kb on - strand, within AO356_24170 at 5421.866 kb on - strand, within AO356_24170 at 5421.866 kb on - strand, within AO356_24170 at 5421.883 kb on + strand, within AO356_24170 at 5421.968 kb on + strand, within AO356_24170 at 5421.980 kb on + strand, within AO356_24170 at 5421.981 kb on - strand, within AO356_24170 at 5421.981 kb on - strand, within AO356_24170 at 5421.981 kb on - strand, within AO356_24170 at 5421.981 kb on - strand, within AO356_24170 at 5422.047 kb on - strand, within AO356_24170 at 5422.047 kb on - strand, within AO356_24170 at 5422.241 kb on + strand, within AO356_24170 at 5422.241 kb on + strand, within AO356_24170 at 5422.242 kb on - strand, within AO356_24170 at 5422.242 kb on - strand, within AO356_24170 at 5422.386 kb on + strand at 5422.430 kb on + strand at 5422.430 kb on + strand at 5422.431 kb on - strand at 5422.511 kb on + strand at 5422.511 kb on + strand at 5422.512 kb on - strand at 5422.512 kb on - strand at 5422.512 kb on - strand at 5422.537 kb on - strand at 5422.690 kb on + strand, within AO356_24175 at 5422.690 kb on + strand, within AO356_24175 at 5422.690 kb on + strand, within AO356_24175 at 5422.735 kb on + strand, within AO356_24175 at 5422.735 kb on + strand, within AO356_24175 at 5422.736 kb on - strand, within AO356_24175 at 5422.736 kb on - strand, within AO356_24175 at 5422.736 kb on - strand, within AO356_24175 at 5422.864 kb on + strand at 5422.947 kb on - strand at 5422.947 kb on - strand at 5422.957 kb on + strand at 5422.957 kb on + strand at 5422.957 kb on + strand at 5422.978 kb on + strand at 5422.978 kb on + strand at 5422.979 kb on - strand at 5422.979 kb on - strand at 5423.048 kb on - strand, within AO356_24180 at 5423.090 kb on + strand, within AO356_24180 at 5423.090 kb on + strand, within AO356_24180 at 5423.091 kb on - strand, within AO356_24180 at 5423.091 kb on - strand, within AO356_24180 at 5423.092 kb on + strand, within AO356_24180 at 5423.092 kb on + strand, within AO356_24180 at 5423.092 kb on + strand, within AO356_24180 at 5423.092 kb on + strand, within AO356_24180 at 5423.092 kb on + strand, within AO356_24180 at 5423.092 kb on + strand, within AO356_24180 at 5423.093 kb on - strand, within AO356_24180 at 5423.093 kb on - strand, within AO356_24180 at 5423.140 kb on + strand, within AO356_24180 at 5423.197 kb on + strand, within AO356_24180 at 5423.197 kb on + strand, within AO356_24180 at 5423.198 kb on - strand, within AO356_24180 at 5423.198 kb on - strand, within AO356_24180 at 5423.307 kb on + strand, within AO356_24180 at 5423.307 kb on + strand, within AO356_24180 at 5423.579 kb on + strand at 5423.580 kb on - strand at 5423.594 kb on + strand at 5423.594 kb on + strand at 5423.595 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2 mg/ml remove 5,421,696 - AO356_24170 0.67 +0.7 5,421,696 - AO356_24170 0.67 +0.0 5,421,696 - AO356_24170 0.67 +0.2 5,421,866 - AO356_24170 0.73 +1.1 5,421,866 - AO356_24170 0.73 +0.6 5,421,883 + AO356_24170 0.73 -1.7 5,421,968 + AO356_24170 0.76 -0.5 5,421,980 + AO356_24170 0.77 +1.0 5,421,981 - AO356_24170 0.77 -2.1 5,421,981 - AO356_24170 0.77 +0.5 5,421,981 - AO356_24170 0.77 +0.7 5,421,981 - AO356_24170 0.77 -0.6 5,422,047 - AO356_24170 0.79 -0.1 5,422,047 - AO356_24170 0.79 +1.0 5,422,241 + AO356_24170 0.86 -0.2 5,422,241 + AO356_24170 0.86 -0.4 5,422,242 - AO356_24170 0.86 +0.0 5,422,242 - AO356_24170 0.86 -1.3 5,422,386 + +0.5 5,422,430 + -0.5 5,422,430 + +0.1 5,422,431 - -0.7 5,422,511 + +0.9 5,422,511 + +0.3 5,422,512 - -0.7 5,422,512 - +0.9 5,422,512 - -1.1 5,422,537 - +0.4 5,422,690 + AO356_24175 0.20 +0.3 5,422,690 + AO356_24175 0.20 -0.6 5,422,690 + AO356_24175 0.20 -1.4 5,422,735 + AO356_24175 0.41 -0.6 5,422,735 + AO356_24175 0.41 -0.5 5,422,736 - AO356_24175 0.41 -0.9 5,422,736 - AO356_24175 0.41 -0.9 5,422,736 - AO356_24175 0.41 +0.2 5,422,864 + -1.4 5,422,947 - -0.3 5,422,947 - +0.5 5,422,957 + -0.6 5,422,957 + +1.4 5,422,957 + -0.2 5,422,978 + -1.3 5,422,978 + -1.7 5,422,979 - -0.7 5,422,979 - -0.1 5,423,048 - AO356_24180 0.17 +1.2 5,423,090 + AO356_24180 0.25 -1.5 5,423,090 + AO356_24180 0.25 +0.7 5,423,091 - AO356_24180 0.25 +1.0 5,423,091 - AO356_24180 0.25 +0.7 5,423,092 + AO356_24180 0.26 +0.4 5,423,092 + AO356_24180 0.26 +0.5 5,423,092 + AO356_24180 0.26 +0.8 5,423,092 + AO356_24180 0.26 -0.8 5,423,092 + AO356_24180 0.26 +0.5 5,423,092 + AO356_24180 0.26 +0.5 5,423,093 - AO356_24180 0.26 +0.8 5,423,093 - AO356_24180 0.26 +1.9 5,423,140 + AO356_24180 0.35 -0.6 5,423,197 + AO356_24180 0.45 +0.0 5,423,197 + AO356_24180 0.45 +0.8 5,423,198 - AO356_24180 0.46 -0.7 5,423,198 - AO356_24180 0.46 -0.1 5,423,307 + AO356_24180 0.66 +0.6 5,423,307 + AO356_24180 0.66 +0.1 5,423,579 + -0.3 5,423,580 - -0.2 5,423,594 + +0.9 5,423,594 + -0.2 5,423,595 - -0.4
Or see this region's nucleotide sequence