Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3620 and Shew_3621 are separated by 262 nucleotides Shew_3621 and hslO are separated by 13 nucleotides hslO and Shew_3623 are separated by 190 nucleotides
Shew_3620: Shew_3620 - general secretion pathway protein C (RefSeq), at 4,317,974 to 4,318,891
_3620
Shew_3621: Shew_3621 - RNA-binding S4 domain-containing protein (RefSeq), at 4,319,154 to 4,319,546
_3621
Shew_3622: hslO - Hsp33-like chaperonin (RefSeq), at 4,319,560 to 4,320,420
hslO
Shew_3623: Shew_3623 - phosphoenolpyruvate carboxykinase (RefSeq), at 4,320,611 to 4,322,152
_3623
Position (kb)
4319
4320
4321 Strain fitness (log2 ratio)
-1
0
1
2 at 4318.634 kb on - strand, within Shew_3620 at 4318.917 kb on - strand at 4318.924 kb on - strand at 4318.954 kb on + strand at 4318.983 kb on + strand at 4319.046 kb on - strand at 4319.047 kb on + strand at 4319.140 kb on - strand at 4319.218 kb on - strand, within Shew_3621 at 4319.305 kb on - strand, within Shew_3621 at 4319.661 kb on + strand, within hslO at 4319.661 kb on + strand, within hslO at 4319.669 kb on - strand, within hslO at 4319.669 kb on - strand, within hslO at 4320.014 kb on - strand, within hslO at 4320.087 kb on - strand, within hslO at 4320.174 kb on - strand, within hslO at 4320.320 kb on - strand, within hslO
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 4,318,634 - Shew_3620 0.72 -0.2 4,318,917 - +0.0 4,318,924 - +0.1 4,318,954 + -0.7 4,318,983 + +0.1 4,319,046 - +0.4 4,319,047 + +0.1 4,319,140 - +0.1 4,319,218 - Shew_3621 0.16 -0.1 4,319,305 - Shew_3621 0.38 +2.2 4,319,661 + hslO Shew_3622 0.12 +0.9 4,319,661 + hslO Shew_3622 0.12 +0.8 4,319,669 - hslO Shew_3622 0.13 +0.8 4,319,669 - hslO Shew_3622 0.13 +0.7 4,320,014 - hslO Shew_3622 0.53 +1.0 4,320,087 - hslO Shew_3622 0.61 +0.1 4,320,174 - hslO Shew_3622 0.71 +0.6 4,320,320 - hslO Shew_3622 0.88 +1.0
Or see this region's nucleotide sequence