Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3546 and Shew_3547 are separated by 24 nucleotides Shew_3547 and Shew_3548 are separated by 300 nucleotides Shew_3548 and Shew_3549 are separated by 222 nucleotides
Shew_3546: Shew_3546 - hypothetical protein (RefSeq), at 4,233,641 to 4,233,862
_3546
Shew_3547: Shew_3547 - hypothetical protein (RefSeq), at 4,233,887 to 4,234,537
_3547
Shew_3548: Shew_3548 - hypothetical protein (RefSeq), at 4,234,838 to 4,235,455
_3548
Shew_3549: Shew_3549 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 4,235,678 to 4,236,892
_3549
Position (kb) 4234
4235
4236 Strain fitness (log2 ratio) -1
0
1
2 at 4233.847 kb on + strand at 4234.610 kb on + strand at 4234.618 kb on - strand at 4234.663 kb on - strand at 4234.808 kb on + strand at 4235.140 kb on + strand, within Shew_3548 at 4235.306 kb on - strand, within Shew_3548 at 4235.387 kb on - strand, within Shew_3548 at 4235.706 kb on - strand at 4235.708 kb on - strand at 4236.021 kb on + strand, within Shew_3549 at 4236.345 kb on + strand, within Shew_3549
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 4,233,847 + -0.1 4,234,610 + -0.4 4,234,618 - +0.1 4,234,663 - +0.1 4,234,808 + -0.1 4,235,140 + Shew_3548 0.49 -0.0 4,235,306 - Shew_3548 0.76 -0.6 4,235,387 - Shew_3548 0.89 +2.2 4,235,706 - +0.3 4,235,708 - +0.3 4,236,021 + Shew_3549 0.28 +0.2 4,236,345 + Shew_3549 0.55 -0.3
Or see this region's nucleotide sequence