Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3061 and Shew_3062 are separated by 568 nucleotides Shew_3062 and Shew_3063 are separated by 168 nucleotides Shew_3063 and gcvT are separated by 193 nucleotides
Shew_3061: Shew_3061 - hypothetical protein (RefSeq), at 3,634,107 to 3,634,916
_3061
Shew_3062: Shew_3062 - glycine dehydrogenase (RefSeq), at 3,635,485 to 3,638,373
_3062
Shew_3063: Shew_3063 - glycine cleavage system protein H (RefSeq), at 3,638,542 to 3,638,931
_3063
Shew_3064: gcvT - glycine cleavage system aminomethyltransferase T (RefSeq), at 3,639,125 to 3,640,219
gcvT
Position (kb)
3635
3636
3637
3638
3639 Strain fitness (log2 ratio)
-1
0
1 at 3634.880 kb on - strand at 3634.942 kb on - strand at 3635.017 kb on - strand at 3635.121 kb on - strand at 3635.214 kb on + strand at 3635.418 kb on + strand at 3635.513 kb on - strand at 3635.620 kb on + strand at 3635.667 kb on + strand at 3635.782 kb on - strand, within Shew_3062 at 3635.960 kb on - strand, within Shew_3062 at 3635.965 kb on - strand, within Shew_3062 at 3636.255 kb on + strand, within Shew_3062 at 3636.295 kb on + strand, within Shew_3062 at 3636.510 kb on - strand, within Shew_3062 at 3636.742 kb on - strand, within Shew_3062 at 3637.424 kb on + strand, within Shew_3062 at 3637.424 kb on + strand, within Shew_3062 at 3637.432 kb on - strand, within Shew_3062 at 3637.432 kb on - strand, within Shew_3062 at 3637.696 kb on + strand, within Shew_3062 at 3637.730 kb on + strand, within Shew_3062 at 3637.748 kb on - strand, within Shew_3062 at 3637.883 kb on + strand, within Shew_3062 at 3638.076 kb on + strand, within Shew_3062 at 3638.096 kb on - strand at 3638.114 kb on - strand at 3638.199 kb on + strand at 3638.864 kb on - strand, within Shew_3063 at 3639.113 kb on - strand at 3639.136 kb on + strand at 3639.267 kb on - strand, within gcvT at 3639.306 kb on - strand, within gcvT at 3639.359 kb on - strand, within gcvT
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 3,634,880 - +0.3 3,634,942 - +0.2 3,635,017 - +0.0 3,635,121 - -0.2 3,635,214 + -0.3 3,635,418 + -0.0 3,635,513 - +0.4 3,635,620 + +1.1 3,635,667 + +0.6 3,635,782 - Shew_3062 0.10 +0.4 3,635,960 - Shew_3062 0.16 +0.6 3,635,965 - Shew_3062 0.17 +0.5 3,636,255 + Shew_3062 0.27 +0.9 3,636,295 + Shew_3062 0.28 +0.6 3,636,510 - Shew_3062 0.35 +0.6 3,636,742 - Shew_3062 0.44 +0.6 3,637,424 + Shew_3062 0.67 +0.7 3,637,424 + Shew_3062 0.67 +0.0 3,637,432 - Shew_3062 0.67 +0.6 3,637,432 - Shew_3062 0.67 +0.5 3,637,696 + Shew_3062 0.77 +0.5 3,637,730 + Shew_3062 0.78 +1.0 3,637,748 - Shew_3062 0.78 +0.6 3,637,883 + Shew_3062 0.83 +0.8 3,638,076 + Shew_3062 0.90 +0.7 3,638,096 - +0.7 3,638,114 - +0.5 3,638,199 + +0.8 3,638,864 - Shew_3063 0.83 +0.3 3,639,113 - +0.5 3,639,136 + +0.0 3,639,267 - gcvT Shew_3064 0.13 +0.3 3,639,306 - gcvT Shew_3064 0.17 +0.7 3,639,359 - gcvT Shew_3064 0.21 +0.1
Or see this region's nucleotide sequence