Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflA and Shew_2392 are separated by 6 nucleotides Shew_2392 and Shew_2393 are separated by 195 nucleotides Shew_2393 and Shew_2394 are separated by 96 nucleotides
Shew_2391: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 2,804,882 to 2,805,622
pflA
Shew_2392: Shew_2392 - hypothetical protein (RefSeq), at 2,805,629 to 2,806,072
_2392
Shew_2393: Shew_2393 - acetate kinase (RefSeq), at 2,806,268 to 2,807,464
_2393
Shew_2394: Shew_2394 - phosphate acetyltransferase (RefSeq), at 2,807,561 to 2,809,699
_2394
Position (kb)
2806
2807
2808 Strain fitness (log2 ratio)
-1
0
1 at 2806.022 kb on - strand, within Shew_2392 at 2806.107 kb on - strand at 2806.197 kb on + strand at 2806.370 kb on - strand at 2806.638 kb on + strand, within Shew_2393 at 2806.705 kb on + strand, within Shew_2393 at 2806.983 kb on - strand, within Shew_2393 at 2807.276 kb on + strand, within Shew_2393 at 2807.312 kb on + strand, within Shew_2393 at 2807.579 kb on - strand at 2807.888 kb on + strand, within Shew_2394 at 2808.260 kb on - strand, within Shew_2394 at 2808.260 kb on - strand, within Shew_2394 at 2808.437 kb on - strand, within Shew_2394
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 2,806,022 - Shew_2392 0.89 -0.2 2,806,107 - -1.1 2,806,197 + -0.1 2,806,370 - +0.3 2,806,638 + Shew_2393 0.31 -0.4 2,806,705 + Shew_2393 0.37 -0.3 2,806,983 - Shew_2393 0.60 -0.4 2,807,276 + Shew_2393 0.84 -0.6 2,807,312 + Shew_2393 0.87 -0.5 2,807,579 - -0.2 2,807,888 + Shew_2394 0.15 -0.2 2,808,260 - Shew_2394 0.33 +0.2 2,808,260 - Shew_2394 0.33 -0.4 2,808,437 - Shew_2394 0.41 +1.1
Or see this region's nucleotide sequence