Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1300 and Shew_1301 are separated by 325 nucleotides
Shew_1300: Shew_1300 - lytic transglycosylase, catalytic (RefSeq), at 1,502,607 to 1,504,037
_1300
Shew_1301: Shew_1301 - phosphoribosylformylglycinamidine synthase (RefSeq), at 1,504,363 to 1,508,244
_1301
Position (kb)
1504
1505
1506
1507
1508
1509 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1503.404 kb on + strand, within Shew_1300 at 1503.711 kb on - strand, within Shew_1300 at 1503.758 kb on - strand, within Shew_1300 at 1503.895 kb on + strand at 1503.925 kb on - strand at 1504.265 kb on + strand at 1504.427 kb on + strand at 1504.546 kb on - strand at 1504.578 kb on + strand at 1504.836 kb on - strand, within Shew_1301 at 1504.883 kb on + strand, within Shew_1301 at 1504.883 kb on + strand, within Shew_1301 at 1505.264 kb on + strand, within Shew_1301 at 1505.874 kb on - strand, within Shew_1301 at 1505.935 kb on - strand, within Shew_1301 at 1506.110 kb on + strand, within Shew_1301 at 1506.610 kb on + strand, within Shew_1301 at 1506.610 kb on + strand, within Shew_1301 at 1506.803 kb on + strand, within Shew_1301 at 1506.859 kb on - strand, within Shew_1301 at 1506.897 kb on + strand, within Shew_1301 at 1506.897 kb on + strand, within Shew_1301 at 1506.897 kb on + strand, within Shew_1301 at 1506.897 kb on + strand, within Shew_1301 at 1507.005 kb on - strand, within Shew_1301 at 1507.035 kb on - strand, within Shew_1301 at 1507.150 kb on - strand, within Shew_1301 at 1507.654 kb on - strand, within Shew_1301 at 1508.092 kb on - strand at 1508.097 kb on + strand at 1508.148 kb on + strand at 1508.233 kb on + strand at 1508.622 kb on - strand at 1508.785 kb on + strand at 1508.785 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,503,404 + Shew_1300 0.56 -0.6 1,503,711 - Shew_1300 0.77 +0.5 1,503,758 - Shew_1300 0.80 +0.8 1,503,895 + +0.3 1,503,925 - +0.6 1,504,265 + +1.0 1,504,427 + +2.1 1,504,546 - +2.1 1,504,578 + +2.9 1,504,836 - Shew_1301 0.12 +2.3 1,504,883 + Shew_1301 0.13 +2.1 1,504,883 + Shew_1301 0.13 +2.6 1,505,264 + Shew_1301 0.23 +2.0 1,505,874 - Shew_1301 0.39 +2.1 1,505,935 - Shew_1301 0.40 +2.0 1,506,110 + Shew_1301 0.45 +1.8 1,506,610 + Shew_1301 0.58 +2.1 1,506,610 + Shew_1301 0.58 +1.7 1,506,803 + Shew_1301 0.63 +2.0 1,506,859 - Shew_1301 0.64 +2.1 1,506,897 + Shew_1301 0.65 +2.1 1,506,897 + Shew_1301 0.65 +2.1 1,506,897 + Shew_1301 0.65 +2.3 1,506,897 + Shew_1301 0.65 +2.7 1,507,005 - Shew_1301 0.68 +1.5 1,507,035 - Shew_1301 0.69 +2.1 1,507,150 - Shew_1301 0.72 +2.2 1,507,654 - Shew_1301 0.85 +2.7 1,508,092 - +2.3 1,508,097 + +2.1 1,508,148 + +2.2 1,508,233 + +2.0 1,508,622 - -0.2 1,508,785 + -1.0 1,508,785 + -0.5
Or see this region's nucleotide sequence