Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1184 and glyA are separated by 342 nucleotides glyA and Shew_1186 are separated by 124 nucleotides Shew_1186 and Shew_1187 are separated by 38 nucleotides
Shew_1184: Shew_1184 - hypothetical protein (RefSeq), at 1,375,662 to 1,377,287
_1184
Shew_1185: glyA - serine hydroxymethyltransferase (RefSeq), at 1,377,630 to 1,378,883
glyA
Shew_1186: Shew_1186 - transcriptional regulator NrdR (RefSeq), at 1,379,008 to 1,379,481
_1186
Shew_1187: Shew_1187 - riboflavin biosynthesis protein RibD (RefSeq), at 1,379,520 to 1,380,680
_1187
Position (kb)
1377
1378
1379 Strain fitness (log2 ratio)
-1
0
1 at 1376.718 kb on + strand, within Shew_1184 at 1376.718 kb on + strand, within Shew_1184 at 1376.742 kb on - strand, within Shew_1184 at 1376.896 kb on - strand, within Shew_1184 at 1376.962 kb on + strand, within Shew_1184 at 1376.962 kb on + strand, within Shew_1184 at 1376.970 kb on - strand, within Shew_1184 at 1376.970 kb on - strand, within Shew_1184 at 1377.148 kb on + strand at 1377.689 kb on + strand at 1377.929 kb on + strand, within glyA at 1377.937 kb on - strand, within glyA at 1378.126 kb on + strand, within glyA at 1378.325 kb on - strand, within glyA at 1378.669 kb on + strand, within glyA at 1379.140 kb on + strand, within Shew_1186
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,376,718 + Shew_1184 0.65 +0.1 1,376,718 + Shew_1184 0.65 +0.1 1,376,742 - Shew_1184 0.66 -0.1 1,376,896 - Shew_1184 0.76 -0.1 1,376,962 + Shew_1184 0.80 +0.1 1,376,962 + Shew_1184 0.80 +0.1 1,376,970 - Shew_1184 0.80 +0.2 1,376,970 - Shew_1184 0.80 +0.3 1,377,148 + +0.1 1,377,689 + +0.9 1,377,929 + glyA Shew_1185 0.24 +0.8 1,377,937 - glyA Shew_1185 0.24 +1.3 1,378,126 + glyA Shew_1185 0.40 +0.5 1,378,325 - glyA Shew_1185 0.55 +0.6 1,378,669 + glyA Shew_1185 0.83 +0.9 1,379,140 + Shew_1186 0.28 +0.6
Or see this region's nucleotide sequence