Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1018 and Shew_1019 are separated by 60 nucleotides Shew_1019 and anmK are separated by 142 nucleotides anmK and Shew_1021 are separated by 37 nucleotides
Shew_1018: Shew_1018 - hypothetical protein (RefSeq), at 1,177,601 to 1,178,074
_1018
Shew_1019: Shew_1019 - hypothetical protein (RefSeq), at 1,178,135 to 1,178,521
_1019
Shew_1020: anmK - anhydro-N-acetylmuramic acid kinase (RefSeq), at 1,178,664 to 1,179,773
anmK
Shew_1021: Shew_1021 - peptidase M23B (RefSeq), at 1,179,811 to 1,181,286
_1021
Position (kb)
1178
1179
1180 Strain fitness (log2 ratio)
-1
0
1 at 1177.713 kb on - strand, within Shew_1018 at 1177.744 kb on + strand, within Shew_1018 at 1178.060 kb on - strand at 1178.154 kb on - strand at 1178.298 kb on - strand, within Shew_1019 at 1178.438 kb on - strand, within Shew_1019 at 1178.576 kb on - strand at 1178.603 kb on + strand at 1178.644 kb on + strand at 1178.841 kb on - strand, within anmK at 1178.894 kb on + strand, within anmK at 1179.022 kb on - strand, within anmK at 1179.833 kb on + strand at 1179.833 kb on + strand at 1180.005 kb on + strand, within Shew_1021 at 1180.228 kb on - strand, within Shew_1021 at 1180.228 kb on - strand, within Shew_1021 at 1180.228 kb on - strand, within Shew_1021 at 1180.242 kb on + strand, within Shew_1021 at 1180.265 kb on - strand, within Shew_1021 at 1180.436 kb on + strand, within Shew_1021
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,177,713 - Shew_1018 0.24 -1.1 1,177,744 + Shew_1018 0.30 -0.2 1,178,060 - +0.2 1,178,154 - -0.3 1,178,298 - Shew_1019 0.42 +0.2 1,178,438 - Shew_1019 0.78 -0.6 1,178,576 - -0.3 1,178,603 + +0.2 1,178,644 + +0.3 1,178,841 - anmK Shew_1020 0.16 -1.0 1,178,894 + anmK Shew_1020 0.21 -0.2 1,179,022 - anmK Shew_1020 0.32 -0.5 1,179,833 + +0.2 1,179,833 + -0.1 1,180,005 + Shew_1021 0.13 -0.1 1,180,228 - Shew_1021 0.28 +0.3 1,180,228 - Shew_1021 0.28 +0.2 1,180,228 - Shew_1021 0.28 +0.2 1,180,242 + Shew_1021 0.29 -0.0 1,180,265 - Shew_1021 0.31 -0.2 1,180,436 + Shew_1021 0.42 -0.1
Or see this region's nucleotide sequence