Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metF and Shew_0523 are separated by 124 nucleotides Shew_0523 and Shew_0524 are separated by 10 nucleotides
Shew_0522: metF - 5,10-methylenetetrahydrofolate reductase (RefSeq), at 593,019 to 593,909
metF
Shew_0523: Shew_0523 - cytochrome c family protein (RefSeq), at 594,034 to 594,633
_0523
Shew_0524: Shew_0524 - cytochrome c, class I (RefSeq), at 594,644 to 595,705
_0524
Position (kb) 594
595 Strain fitness (log2 ratio) -2
-1
0
1 at 593.299 kb on - strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.400 kb on - strand, within metF at 593.400 kb on - strand, within metF at 593.400 kb on - strand, within metF at 593.400 kb on - strand, within metF at 593.446 kb on - strand, within metF at 593.746 kb on + strand, within metF at 593.844 kb on + strand at 593.844 kb on + strand at 593.845 kb on - strand at 593.852 kb on - strand at 593.886 kb on - strand at 594.027 kb on + strand at 594.128 kb on - strand, within Shew_0523 at 594.337 kb on - strand, within Shew_0523 at 594.400 kb on - strand, within Shew_0523 at 594.465 kb on + strand, within Shew_0523 at 594.895 kb on - strand, within Shew_0524 at 594.922 kb on + strand, within Shew_0524 at 595.132 kb on + strand, within Shew_0524 at 595.177 kb on + strand, within Shew_0524 at 595.378 kb on - strand, within Shew_0524 at 595.388 kb on + strand, within Shew_0524 at 595.488 kb on - strand, within Shew_0524 at 595.492 kb on + strand, within Shew_0524 at 595.492 kb on + strand, within Shew_0524 at 595.492 kb on + strand, within Shew_0524 at 595.500 kb on - strand, within Shew_0524 at 595.500 kb on + strand, within Shew_0524 at 595.500 kb on - strand, within Shew_0524 at 595.530 kb on - strand, within Shew_0524
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 593,299 - metF Shew_0522 0.31 +0.3 593,392 + metF Shew_0522 0.42 -0.2 593,392 + metF Shew_0522 0.42 +0.3 593,392 + metF Shew_0522 0.42 -0.1 593,392 + metF Shew_0522 0.42 -0.2 593,392 + metF Shew_0522 0.42 +0.1 593,392 + metF Shew_0522 0.42 +0.2 593,392 + metF Shew_0522 0.42 +0.0 593,400 - metF Shew_0522 0.43 -0.1 593,400 - metF Shew_0522 0.43 -0.1 593,400 - metF Shew_0522 0.43 +0.0 593,400 - metF Shew_0522 0.43 -2.2 593,446 - metF Shew_0522 0.48 -0.6 593,746 + metF Shew_0522 0.82 -0.2 593,844 + +0.1 593,844 + -0.0 593,845 - +0.2 593,852 - -0.1 593,886 - -0.1 594,027 + +0.2 594,128 - Shew_0523 0.16 -0.5 594,337 - Shew_0523 0.51 -0.6 594,400 - Shew_0523 0.61 -0.6 594,465 + Shew_0523 0.72 +0.2 594,895 - Shew_0524 0.24 +0.1 594,922 + Shew_0524 0.26 -0.4 595,132 + Shew_0524 0.46 +0.5 595,177 + Shew_0524 0.50 -0.3 595,378 - Shew_0524 0.69 -0.1 595,388 + Shew_0524 0.70 +0.0 595,488 - Shew_0524 0.79 +0.3 595,492 + Shew_0524 0.80 +0.0 595,492 + Shew_0524 0.80 +0.0 595,492 + Shew_0524 0.80 -0.1 595,500 - Shew_0524 0.81 +0.0 595,500 + Shew_0524 0.81 -0.3 595,500 - Shew_0524 0.81 +0.2 595,530 - Shew_0524 0.83 -0.0
Or see this region's nucleotide sequence