Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0447 and Shew_0448 overlap by 17 nucleotides Shew_0448 and Shew_0449 are separated by 60 nucleotides Shew_0449 and Shew_0450 are separated by 235 nucleotides Shew_0450 and Shew_0451 are separated by 24 nucleotides
Shew_0447: Shew_0447 - hypothetical protein (RefSeq), at 497,998 to 499,203
_0447
Shew_0448: Shew_0448 - periplasmic solute binding protein (RefSeq), at 499,187 to 500,098
_0448
Shew_0449: Shew_0449 - ABC-3 protein (RefSeq), at 500,159 to 500,950
_0449
Shew_0450: Shew_0450 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (RefSeq), at 501,186 to 501,920
_0450
Shew_0451: Shew_0451 - hypothetical protein (RefSeq), at 501,945 to 502,352
_0451
Position (kb) 500
501 Strain fitness (log2 ratio) -1
0
1 at 499.378 kb on - strand, within Shew_0448 at 499.542 kb on + strand, within Shew_0448 at 499.644 kb on - strand, within Shew_0448 at 499.700 kb on + strand, within Shew_0448 at 500.006 kb on - strand, within Shew_0448 at 500.331 kb on - strand, within Shew_0449 at 500.575 kb on - strand, within Shew_0449 at 500.708 kb on - strand, within Shew_0449 at 500.902 kb on + strand at 501.451 kb on + strand, within Shew_0450 at 501.459 kb on - strand, within Shew_0450 at 501.462 kb on + strand, within Shew_0450
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 499,378 - Shew_0448 0.21 +0.1 499,542 + Shew_0448 0.39 -0.1 499,644 - Shew_0448 0.50 +0.6 499,700 + Shew_0448 0.56 +0.1 500,006 - Shew_0448 0.90 -0.1 500,331 - Shew_0449 0.22 -1.4 500,575 - Shew_0449 0.53 -0.0 500,708 - Shew_0449 0.69 -0.1 500,902 + +0.2 501,451 + Shew_0450 0.36 +0.1 501,459 - Shew_0450 0.37 -0.3 501,462 + Shew_0450 0.38 +0.1
Or see this region's nucleotide sequence