Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0209 and Shew_0210 overlap by 4 nucleotides Shew_0210 and Shew_0211 overlap by 4 nucleotides Shew_0211 and Shew_0212 are separated by 22 nucleotides
Shew_0209: Shew_0209 - fimbrial assembly family protein (RefSeq), at 224,781 to 225,362
_0209
Shew_0210: Shew_0210 - pilus assembly protein, PilO (RefSeq), at 225,359 to 225,982
_0210
Shew_0211: Shew_0211 - pilus assembly protein, PilQ (RefSeq), at 225,979 to 226,494
_0211
Shew_0212: Shew_0212 - type IV pilus secretin PilQ (RefSeq), at 226,517 to 228,568
_0212
Position (kb) 225
226
227 Strain fitness (log2 ratio) -2
-1
0
1 at 225.076 kb on + strand, within Shew_0209 at 225.084 kb on - strand, within Shew_0209 at 225.424 kb on + strand, within Shew_0210 at 225.424 kb on + strand, within Shew_0210 at 225.432 kb on - strand, within Shew_0210 at 225.516 kb on - strand, within Shew_0210 at 225.516 kb on - strand, within Shew_0210 at 225.516 kb on - strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.710 kb on - strand, within Shew_0210 at 225.710 kb on - strand, within Shew_0210 at 225.825 kb on - strand, within Shew_0210 at 225.837 kb on - strand, within Shew_0210 at 226.204 kb on + strand, within Shew_0211 at 226.271 kb on + strand, within Shew_0211 at 226.289 kb on - strand, within Shew_0211 at 226.380 kb on - strand, within Shew_0211 at 226.480 kb on - strand at 226.480 kb on - strand at 226.572 kb on - strand at 226.572 kb on - strand at 226.620 kb on - strand at 226.628 kb on + strand at 226.640 kb on + strand at 226.821 kb on + strand, within Shew_0212 at 226.824 kb on - strand, within Shew_0212 at 226.844 kb on - strand, within Shew_0212 at 226.854 kb on - strand, within Shew_0212 at 226.873 kb on + strand, within Shew_0212 at 226.916 kb on - strand, within Shew_0212 at 227.076 kb on + strand, within Shew_0212 at 227.096 kb on - strand, within Shew_0212 at 227.141 kb on + strand, within Shew_0212 at 227.141 kb on + strand, within Shew_0212 at 227.358 kb on - strand, within Shew_0212 at 227.423 kb on + strand, within Shew_0212
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 225,076 + Shew_0209 0.51 -0.1 225,084 - Shew_0209 0.52 +0.2 225,424 + Shew_0210 0.10 +0.1 225,424 + Shew_0210 0.10 +0.0 225,432 - Shew_0210 0.12 -0.2 225,516 - Shew_0210 0.25 -0.1 225,516 - Shew_0210 0.25 -0.2 225,516 - Shew_0210 0.25 +0.1 225,702 + Shew_0210 0.55 +0.1 225,702 + Shew_0210 0.55 +0.4 225,702 + Shew_0210 0.55 -0.5 225,710 - Shew_0210 0.56 +0.0 225,710 - Shew_0210 0.56 +0.1 225,825 - Shew_0210 0.75 -0.3 225,837 - Shew_0210 0.77 +0.1 226,204 + Shew_0211 0.44 -0.0 226,271 + Shew_0211 0.57 +0.3 226,289 - Shew_0211 0.60 +1.4 226,380 - Shew_0211 0.78 +0.2 226,480 - -0.1 226,480 - +0.1 226,572 - -0.0 226,572 - -0.1 226,620 - +1.3 226,628 + +0.1 226,640 + -0.1 226,821 + Shew_0212 0.15 +0.1 226,824 - Shew_0212 0.15 +0.1 226,844 - Shew_0212 0.16 +0.1 226,854 - Shew_0212 0.16 -2.0 226,873 + Shew_0212 0.17 -0.0 226,916 - Shew_0212 0.19 +0.7 227,076 + Shew_0212 0.27 -0.6 227,096 - Shew_0212 0.28 +0.0 227,141 + Shew_0212 0.30 +0.1 227,141 + Shew_0212 0.30 -1.2 227,358 - Shew_0212 0.41 +0.2 227,423 + Shew_0212 0.44 -0.0
Or see this region's nucleotide sequence