Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4138 and Dshi_4139 overlap by 8 nucleotides Dshi_4139 and Dshi_4140 are separated by 46 nucleotides Dshi_4140 and Dshi_4141 are separated by 212 nucleotides
Dshi_4138: Dshi_4138 - hypothetical protein (RefSeq), at 70,715 to 71,707
_4138
Dshi_4139: Dshi_4139 - GDP-mannose 4,6-dehydratase (RefSeq), at 71,700 to 72,764
_4139
Dshi_4140: Dshi_4140 - sulfotransferase (RefSeq), at 72,811 to 73,542
_4140
Dshi_4141: Dshi_4141 - hypothetical protein (RefSeq), at 73,755 to 74,459
_4141
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 70.713 kb on + strand at 70.770 kb on + strand at 70.778 kb on - strand at 70.793 kb on - strand at 70.934 kb on + strand, within Dshi_4138 at 70.942 kb on - strand, within Dshi_4138 at 70.993 kb on - strand, within Dshi_4138 at 71.061 kb on - strand, within Dshi_4138 at 71.128 kb on - strand, within Dshi_4138 at 71.218 kb on - strand, within Dshi_4138 at 71.223 kb on + strand, within Dshi_4138 at 71.269 kb on + strand, within Dshi_4138 at 71.374 kb on + strand, within Dshi_4138 at 71.436 kb on - strand, within Dshi_4138 at 71.589 kb on + strand, within Dshi_4138 at 71.643 kb on + strand at 71.943 kb on + strand, within Dshi_4139 at 72.008 kb on - strand, within Dshi_4139 at 72.128 kb on - strand, within Dshi_4139 at 72.277 kb on + strand, within Dshi_4139 at 72.285 kb on - strand, within Dshi_4139 at 72.285 kb on - strand, within Dshi_4139 at 72.285 kb on - strand, within Dshi_4139 at 72.351 kb on - strand, within Dshi_4139 at 72.369 kb on + strand, within Dshi_4139 at 72.475 kb on - strand, within Dshi_4139 at 72.498 kb on + strand, within Dshi_4139 at 72.555 kb on - strand, within Dshi_4139 at 72.557 kb on + strand, within Dshi_4139 at 72.631 kb on + strand, within Dshi_4139 at 72.683 kb on + strand at 72.683 kb on + strand at 72.691 kb on - strand at 72.699 kb on - strand at 72.721 kb on + strand at 72.721 kb on + strand at 72.731 kb on + strand at 72.748 kb on - strand at 72.776 kb on - strand at 72.810 kb on - strand at 72.820 kb on - strand at 72.834 kb on - strand at 72.917 kb on - strand, within Dshi_4140 at 72.982 kb on - strand, within Dshi_4140 at 72.982 kb on - strand, within Dshi_4140 at 73.024 kb on + strand, within Dshi_4140 at 73.063 kb on + strand, within Dshi_4140 at 73.071 kb on - strand, within Dshi_4140 at 73.083 kb on + strand, within Dshi_4140 at 73.089 kb on + strand, within Dshi_4140 at 73.108 kb on + strand, within Dshi_4140 at 73.127 kb on - strand, within Dshi_4140 at 73.137 kb on - strand, within Dshi_4140 at 73.191 kb on + strand, within Dshi_4140 at 73.228 kb on + strand, within Dshi_4140 at 73.228 kb on + strand, within Dshi_4140 at 73.229 kb on - strand, within Dshi_4140 at 73.230 kb on - strand, within Dshi_4140 at 73.244 kb on + strand, within Dshi_4140 at 73.264 kb on - strand, within Dshi_4140 at 73.356 kb on + strand, within Dshi_4140 at 73.358 kb on + strand, within Dshi_4140 at 73.399 kb on + strand, within Dshi_4140 at 73.399 kb on + strand, within Dshi_4140 at 73.407 kb on - strand, within Dshi_4140 at 73.409 kb on + strand, within Dshi_4140 at 73.409 kb on + strand, within Dshi_4140 at 73.417 kb on - strand, within Dshi_4140 at 73.417 kb on - strand, within Dshi_4140 at 73.473 kb on - strand at 73.484 kb on - strand at 73.537 kb on - strand at 73.553 kb on + strand at 73.561 kb on - strand at 73.623 kb on + strand at 73.694 kb on + strand at 73.694 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 70,713 + +0.4 70,770 + -0.3 70,778 - -0.6 70,793 - +1.0 70,934 + Dshi_4138 0.22 -0.6 70,942 - Dshi_4138 0.23 +0.6 70,993 - Dshi_4138 0.28 -0.1 71,061 - Dshi_4138 0.35 +0.2 71,128 - Dshi_4138 0.42 -0.1 71,218 - Dshi_4138 0.51 -0.4 71,223 + Dshi_4138 0.51 -0.1 71,269 + Dshi_4138 0.56 +0.9 71,374 + Dshi_4138 0.66 +0.4 71,436 - Dshi_4138 0.73 -1.0 71,589 + Dshi_4138 0.88 +1.6 71,643 + -3.1 71,943 + Dshi_4139 0.23 -2.0 72,008 - Dshi_4139 0.29 -1.4 72,128 - Dshi_4139 0.40 +0.2 72,277 + Dshi_4139 0.54 -1.1 72,285 - Dshi_4139 0.55 -1.3 72,285 - Dshi_4139 0.55 +0.3 72,285 - Dshi_4139 0.55 -0.5 72,351 - Dshi_4139 0.61 +1.8 72,369 + Dshi_4139 0.63 -1.4 72,475 - Dshi_4139 0.73 -3.0 72,498 + Dshi_4139 0.75 -0.4 72,555 - Dshi_4139 0.80 +0.1 72,557 + Dshi_4139 0.80 +1.0 72,631 + Dshi_4139 0.87 -1.4 72,683 + +0.2 72,683 + -0.6 72,691 - -1.1 72,699 - -0.7 72,721 + +0.3 72,721 + -0.9 72,731 + -3.6 72,748 - +1.0 72,776 - -0.2 72,810 - -1.0 72,820 - +0.4 72,834 - -2.1 72,917 - Dshi_4140 0.14 -0.6 72,982 - Dshi_4140 0.23 -1.1 72,982 - Dshi_4140 0.23 -0.1 73,024 + Dshi_4140 0.29 +0.4 73,063 + Dshi_4140 0.34 +0.0 73,071 - Dshi_4140 0.36 +0.1 73,083 + Dshi_4140 0.37 -0.6 73,089 + Dshi_4140 0.38 -1.2 73,108 + Dshi_4140 0.41 +0.6 73,127 - Dshi_4140 0.43 +1.0 73,137 - Dshi_4140 0.45 +0.0 73,191 + Dshi_4140 0.52 -0.6 73,228 + Dshi_4140 0.57 -0.5 73,228 + Dshi_4140 0.57 -0.8 73,229 - Dshi_4140 0.57 -0.8 73,230 - Dshi_4140 0.57 -1.2 73,244 + Dshi_4140 0.59 -0.8 73,264 - Dshi_4140 0.62 -0.9 73,356 + Dshi_4140 0.74 +0.5 73,358 + Dshi_4140 0.75 -0.3 73,399 + Dshi_4140 0.80 -2.8 73,399 + Dshi_4140 0.80 +0.7 73,407 - Dshi_4140 0.81 -0.1 73,409 + Dshi_4140 0.82 -0.6 73,409 + Dshi_4140 0.82 -0.9 73,417 - Dshi_4140 0.83 -0.9 73,417 - Dshi_4140 0.83 -0.2 73,473 - +0.7 73,484 - +0.2 73,537 - -1.0 73,553 + +1.2 73,561 - -1.1 73,623 + -0.2 73,694 + -0.8 73,694 + -0.1
Or see this region's nucleotide sequence