Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3003 and Dshi_3004 are separated by 134 nucleotides Dshi_3004 and Dshi_3005 are separated by 107 nucleotides Dshi_3005 and Dshi_3006 are separated by 0 nucleotides Dshi_3006 and Dshi_3007 are separated by 113 nucleotides
Dshi_3003: Dshi_3003 - TRAP dicarboxylate transporter- DctP subunit (RefSeq), at 3,169,667 to 3,170,650
_3003
Dshi_3004: Dshi_3004 - transcriptional regulator, GntR family (RefSeq), at 3,170,785 to 3,171,441
_3004
Dshi_3005: Dshi_3005 - hypothetical protein (RefSeq), at 3,171,549 to 3,171,857
_3005
Dshi_3006: Dshi_3006 - Ribulose-phosphate 3-epimerase (RefSeq), at 3,171,858 to 3,172,556
_3006
Dshi_3007: Dshi_3007 - Alpha-mannosidase (RefSeq), at 3,172,670 to 3,175,837
_3007
Position (kb)
3171
3172 Strain fitness (log2 ratio)
-1
0
1 at 3170.564 kb on - strand at 3170.581 kb on - strand at 3170.612 kb on + strand at 3170.676 kb on - strand at 3170.788 kb on + strand at 3170.789 kb on - strand at 3170.809 kb on - strand at 3171.044 kb on - strand, within Dshi_3004 at 3171.095 kb on + strand, within Dshi_3004 at 3171.113 kb on - strand, within Dshi_3004 at 3171.151 kb on + strand, within Dshi_3004 at 3171.227 kb on + strand, within Dshi_3004 at 3171.254 kb on - strand, within Dshi_3004 at 3171.305 kb on - strand, within Dshi_3004 at 3171.488 kb on + strand at 3171.491 kb on - strand at 3171.641 kb on + strand, within Dshi_3005 at 3171.655 kb on - strand, within Dshi_3005 at 3171.660 kb on - strand, within Dshi_3005 at 3171.859 kb on - strand at 3171.882 kb on + strand at 3172.666 kb on - strand at 3172.668 kb on + strand at 3172.668 kb on + strand at 3172.676 kb on - strand at 3172.676 kb on - strand at 3172.702 kb on - strand at 3172.732 kb on + strand at 3172.732 kb on + strand at 3172.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,170,564 - +0.2 3,170,581 - -0.1 3,170,612 + -0.9 3,170,676 - +1.2 3,170,788 + -0.6 3,170,789 - +0.0 3,170,809 - +0.4 3,171,044 - Dshi_3004 0.39 -0.0 3,171,095 + Dshi_3004 0.47 -0.3 3,171,113 - Dshi_3004 0.50 -0.0 3,171,151 + Dshi_3004 0.56 -0.1 3,171,227 + Dshi_3004 0.67 -1.6 3,171,254 - Dshi_3004 0.71 -0.5 3,171,305 - Dshi_3004 0.79 +0.3 3,171,488 + -0.0 3,171,491 - +0.1 3,171,641 + Dshi_3005 0.30 -1.2 3,171,655 - Dshi_3005 0.34 +0.1 3,171,660 - Dshi_3005 0.36 -0.5 3,171,859 - -0.1 3,171,882 + +1.5 3,172,666 - +1.2 3,172,668 + -0.0 3,172,668 + -0.5 3,172,676 - -1.2 3,172,676 - -0.3 3,172,702 - +0.4 3,172,732 + -0.6 3,172,732 + +0.2 3,172,732 - -0.7
Or see this region's nucleotide sequence