Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2949 and Dshi_2950 are separated by 141 nucleotides Dshi_2950 and Dshi_2951 are separated by 213 nucleotides Dshi_2951 and Dshi_2952 are separated by 6 nucleotides Dshi_2952 and Dshi_2953 overlap by 4 nucleotides
Dshi_2949: Dshi_2949 - ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent (RefSeq), at 3,109,029 to 3,111,326
_2949
Dshi_2950: Dshi_2950 - hypothetical protein (RefSeq), at 3,111,468 to 3,111,923
_2950
Dshi_2951: Dshi_2951 - Pyridoxal-5'-phosphate-dependent protein beta subunit (RefSeq), at 3,112,137 to 3,113,279
_2951
Dshi_2952: Dshi_2952 - pantoate--beta-alanine ligase (RefSeq), at 3,113,286 to 3,114,131
_2952
Dshi_2953: Dshi_2953 - ATP phosphoribosyltransferase (RefSeq), at 3,114,128 to 3,114,847
_2953
Position (kb)
3112
3113
3114 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3111.162 kb on - strand at 3111.425 kb on + strand at 3111.425 kb on + strand at 3111.554 kb on - strand, within Dshi_2950 at 3111.819 kb on - strand, within Dshi_2950 at 3111.843 kb on + strand, within Dshi_2950 at 3112.125 kb on + strand at 3112.133 kb on - strand at 3112.133 kb on - strand at 3112.220 kb on - strand at 3112.329 kb on - strand, within Dshi_2951 at 3112.360 kb on - strand, within Dshi_2951 at 3112.659 kb on + strand, within Dshi_2951 at 3112.665 kb on - strand, within Dshi_2951 at 3112.884 kb on - strand, within Dshi_2951 at 3112.897 kb on + strand, within Dshi_2951 at 3113.044 kb on - strand, within Dshi_2951 at 3113.062 kb on - strand, within Dshi_2951 at 3113.062 kb on - strand, within Dshi_2951 at 3113.118 kb on - strand, within Dshi_2951 at 3113.120 kb on + strand, within Dshi_2951 at 3113.264 kb on + strand at 3113.288 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,111,162 - -2.2 3,111,425 + -0.5 3,111,425 + -0.2 3,111,554 - Dshi_2950 0.19 -0.1 3,111,819 - Dshi_2950 0.77 +0.1 3,111,843 + Dshi_2950 0.82 -2.5 3,112,125 + -1.1 3,112,133 - -1.4 3,112,133 - +0.6 3,112,220 - -0.5 3,112,329 - Dshi_2951 0.17 -0.4 3,112,360 - Dshi_2951 0.20 -1.2 3,112,659 + Dshi_2951 0.46 -0.1 3,112,665 - Dshi_2951 0.46 +3.6 3,112,884 - Dshi_2951 0.65 -0.7 3,112,897 + Dshi_2951 0.66 -0.9 3,113,044 - Dshi_2951 0.79 -0.3 3,113,062 - Dshi_2951 0.81 +1.2 3,113,062 - Dshi_2951 0.81 -0.3 3,113,118 - Dshi_2951 0.86 +0.6 3,113,120 + Dshi_2951 0.86 +0.2 3,113,264 + +0.2 3,113,288 + -1.2
Or see this region's nucleotide sequence