Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2034 and Dshi_2035 overlap by 8 nucleotides Dshi_2035 and Dshi_2036 overlap by 4 nucleotides Dshi_2036 and Dshi_2037 are separated by 15 nucleotides
Dshi_2034: Dshi_2034 - Histidinol dehydrogenase (RefSeq), at 2,153,718 to 2,155,025
_2034
Dshi_2035: Dshi_2035 - short-chain dehydrogenase/reductase SDR (RefSeq), at 2,155,018 to 2,155,782
_2035
Dshi_2036: Dshi_2036 - Alcohol dehydrogenase GroES domain protein (RefSeq), at 2,155,779 to 2,156,762
_2036
Dshi_2037: Dshi_2037 - hypothetical protein (RefSeq), at 2,156,778 to 2,157,068
_2037
Position (kb)
2155
2156 Strain fitness (log2 ratio)
-2
-1
0
1 at 2154.021 kb on - strand, within Dshi_2034 at 2154.027 kb on - strand, within Dshi_2034 at 2154.188 kb on - strand, within Dshi_2034 at 2154.218 kb on + strand, within Dshi_2034 at 2154.228 kb on + strand, within Dshi_2034 at 2154.453 kb on - strand, within Dshi_2034 at 2154.468 kb on + strand, within Dshi_2034 at 2154.541 kb on + strand, within Dshi_2034 at 2154.547 kb on - strand, within Dshi_2034 at 2154.623 kb on - strand, within Dshi_2034 at 2154.628 kb on - strand, within Dshi_2034 at 2154.675 kb on - strand, within Dshi_2034 at 2154.755 kb on + strand, within Dshi_2034 at 2154.787 kb on - strand, within Dshi_2034 at 2154.862 kb on - strand, within Dshi_2034 at 2154.862 kb on - strand, within Dshi_2034 at 2154.893 kb on + strand, within Dshi_2034 at 2154.893 kb on + strand, within Dshi_2034 at 2154.901 kb on - strand at 2154.948 kb on - strand at 2154.949 kb on + strand at 2154.953 kb on + strand at 2155.008 kb on - strand at 2155.048 kb on + strand at 2155.058 kb on - strand at 2155.294 kb on - strand, within Dshi_2035 at 2155.330 kb on - strand, within Dshi_2035 at 2155.482 kb on - strand, within Dshi_2035 at 2155.514 kb on - strand, within Dshi_2035 at 2155.550 kb on - strand, within Dshi_2035 at 2155.613 kb on - strand, within Dshi_2035 at 2155.636 kb on + strand, within Dshi_2035 at 2155.798 kb on - strand at 2155.865 kb on - strand at 2155.938 kb on - strand, within Dshi_2036 at 2156.071 kb on + strand, within Dshi_2036 at 2156.182 kb on - strand, within Dshi_2036 at 2156.425 kb on - strand at 2156.511 kb on - strand, within Dshi_2036 at 2156.531 kb on - strand, within Dshi_2036 at 2156.556 kb on + strand, within Dshi_2036 at 2156.702 kb on + strand at 2156.710 kb on - strand at 2156.718 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,154,021 - Dshi_2034 0.23 -1.3 2,154,027 - Dshi_2034 0.24 -0.2 2,154,188 - Dshi_2034 0.36 -1.0 2,154,218 + Dshi_2034 0.38 -2.4 2,154,228 + Dshi_2034 0.39 -0.2 2,154,453 - Dshi_2034 0.56 -0.2 2,154,468 + Dshi_2034 0.57 -0.3 2,154,541 + Dshi_2034 0.63 +1.1 2,154,547 - Dshi_2034 0.63 -0.6 2,154,623 - Dshi_2034 0.69 -0.9 2,154,628 - Dshi_2034 0.70 +0.3 2,154,675 - Dshi_2034 0.73 -0.4 2,154,755 + Dshi_2034 0.79 -0.3 2,154,787 - Dshi_2034 0.82 +0.1 2,154,862 - Dshi_2034 0.87 -0.2 2,154,862 - Dshi_2034 0.87 +0.4 2,154,893 + Dshi_2034 0.90 -0.4 2,154,893 + Dshi_2034 0.90 +0.1 2,154,901 - +0.1 2,154,948 - -0.3 2,154,949 + +0.7 2,154,953 + +0.3 2,155,008 - +0.1 2,155,048 + -1.0 2,155,058 - -0.9 2,155,294 - Dshi_2035 0.36 -0.7 2,155,330 - Dshi_2035 0.41 -1.4 2,155,482 - Dshi_2035 0.61 -1.2 2,155,514 - Dshi_2035 0.65 -0.9 2,155,550 - Dshi_2035 0.70 -0.4 2,155,613 - Dshi_2035 0.78 -1.2 2,155,636 + Dshi_2035 0.81 -0.6 2,155,798 - -0.2 2,155,865 - -0.9 2,155,938 - Dshi_2036 0.16 +0.5 2,156,071 + Dshi_2036 0.30 +0.3 2,156,182 - Dshi_2036 0.41 +0.6 2,156,425 - -1.4 2,156,511 - Dshi_2036 0.74 -1.4 2,156,531 - Dshi_2036 0.76 +0.1 2,156,556 + Dshi_2036 0.79 -1.8 2,156,702 + -1.0 2,156,710 - -0.1 2,156,718 + -1.2
Or see this region's nucleotide sequence