Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1158 and Dshi_1159 are separated by 444 nucleotides Dshi_1159 and Dshi_1160 are separated by 4 nucleotides Dshi_1160 and Dshi_1161 are separated by 64 nucleotides
Dshi_1158: Dshi_1158 - Inosine/uridine-preferring nucleoside hydrolase (RefSeq), at 1,184,225 to 1,185,181
_1158
Dshi_1159: Dshi_1159 - hypothetical protein (RefSeq), at 1,185,626 to 1,186,144
_1159
Dshi_1160: Dshi_1160 - MazG family protein (RefSeq), at 1,186,149 to 1,186,988
_1160
Dshi_1161: Dshi_1161 - amidohydrolase (RefSeq), at 1,187,053 to 1,188,213
_1161
Position (kb)
1185
1186
1187 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1184.650 kb on + strand, within Dshi_1158 at 1184.782 kb on + strand, within Dshi_1158 at 1184.815 kb on + strand, within Dshi_1158 at 1184.876 kb on - strand, within Dshi_1158 at 1184.887 kb on + strand, within Dshi_1158 at 1184.990 kb on + strand, within Dshi_1158 at 1185.163 kb on + strand at 1185.163 kb on + strand at 1185.238 kb on - strand at 1185.513 kb on + strand at 1185.521 kb on - strand at 1185.911 kb on - strand, within Dshi_1159 at 1185.990 kb on + strand, within Dshi_1159 at 1185.990 kb on + strand, within Dshi_1159 at 1185.998 kb on - strand, within Dshi_1159 at 1186.065 kb on + strand, within Dshi_1159 at 1186.098 kb on - strand at 1186.103 kb on - strand at 1186.240 kb on + strand, within Dshi_1160 at 1186.487 kb on + strand, within Dshi_1160 at 1186.824 kb on + strand, within Dshi_1160 at 1186.832 kb on - strand, within Dshi_1160 at 1186.832 kb on - strand, within Dshi_1160 at 1186.856 kb on - strand, within Dshi_1160 at 1186.884 kb on + strand, within Dshi_1160 at 1187.019 kb on + strand at 1187.039 kb on + strand at 1187.044 kb on + strand at 1187.087 kb on - strand at 1187.107 kb on - strand at 1187.110 kb on + strand at 1187.123 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,184,650 + Dshi_1158 0.44 +0.5 1,184,782 + Dshi_1158 0.58 +0.4 1,184,815 + Dshi_1158 0.62 +0.8 1,184,876 - Dshi_1158 0.68 -0.1 1,184,887 + Dshi_1158 0.69 -1.1 1,184,990 + Dshi_1158 0.80 -0.1 1,185,163 + -0.6 1,185,163 + +0.1 1,185,238 - -1.6 1,185,513 + +0.1 1,185,521 - -1.0 1,185,911 - Dshi_1159 0.55 -0.5 1,185,990 + Dshi_1159 0.70 -0.0 1,185,990 + Dshi_1159 0.70 +0.9 1,185,998 - Dshi_1159 0.72 +0.7 1,186,065 + Dshi_1159 0.85 +1.9 1,186,098 - +0.2 1,186,103 - +0.8 1,186,240 + Dshi_1160 0.11 +0.2 1,186,487 + Dshi_1160 0.40 +0.8 1,186,824 + Dshi_1160 0.80 -0.4 1,186,832 - Dshi_1160 0.81 +0.4 1,186,832 - Dshi_1160 0.81 +0.9 1,186,856 - Dshi_1160 0.84 +0.2 1,186,884 + Dshi_1160 0.88 -0.3 1,187,019 + -0.3 1,187,039 + -0.0 1,187,044 + -0.8 1,187,087 - -1.0 1,187,107 - -1.8 1,187,110 + -0.4 1,187,123 + +1.3
Or see this region's nucleotide sequence