Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc03067 and SMc03068 are separated by 356 nucleotides SMc03068 and SMc03069 are separated by 91 nucleotides SMc03069 and SMc03070 are separated by 8 nucleotides
SMc03067: SMc03067 - transposase, at 765,114 to 766,277
SMc03067
SMc03068: SMc03068 - phosphogluconate dehydratase, at 766,634 to 768,454
SMc03068
SMc03069: SMc03069 - 6-phosphogluconolactonase, at 768,546 to 769,244
SMc03069
SMc03070: SMc03070 - glucose-6-phosphate 1-dehydrogenase, at 769,253 to 770,728
SMc03070
Position (kb) 766
767
768
769 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 766.437 kb on - strand at 766.437 kb on - strand at 766.522 kb on + strand at 766.539 kb on + strand at 766.545 kb on + strand at 766.545 kb on + strand at 766.545 kb on + strand at 766.545 kb on + strand at 766.545 kb on + strand at 766.546 kb on - strand at 766.546 kb on - strand at 766.546 kb on - strand at 766.546 kb on - strand at 766.546 kb on - strand at 766.636 kb on - strand at 766.636 kb on - strand at 766.808 kb on + strand at 766.834 kb on + strand, within SMc03068 at 766.834 kb on + strand, within SMc03068 at 766.835 kb on - strand, within SMc03068 at 766.912 kb on + strand, within SMc03068 at 767.095 kb on - strand, within SMc03068 at 767.308 kb on - strand, within SMc03068 at 767.494 kb on + strand, within SMc03068 at 767.494 kb on + strand, within SMc03068 at 767.778 kb on + strand, within SMc03068 at 767.785 kb on + strand, within SMc03068 at 767.785 kb on + strand, within SMc03068 at 767.786 kb on - strand, within SMc03068 at 767.805 kb on + strand, within SMc03068 at 767.806 kb on - strand, within SMc03068 at 768.189 kb on + strand, within SMc03068 at 768.189 kb on + strand, within SMc03068 at 768.228 kb on + strand, within SMc03068 at 768.228 kb on + strand, within SMc03068 at 768.229 kb on - strand, within SMc03068 at 768.229 kb on - strand, within SMc03068 at 768.382 kb on - strand at 768.424 kb on + strand at 768.435 kb on - strand at 768.478 kb on + strand at 768.559 kb on + strand at 768.559 kb on + strand at 768.560 kb on - strand at 768.560 kb on - strand at 768.560 kb on - strand at 768.560 kb on - strand at 768.561 kb on + strand at 768.561 kb on + strand at 768.561 kb on + strand at 768.562 kb on - strand at 768.685 kb on + strand, within SMc03069 at 768.827 kb on + strand, within SMc03069 at 768.827 kb on + strand, within SMc03069 at 769.254 kb on + strand at 769.254 kb on + strand at 769.330 kb on + strand at 769.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 766,437 - +0.3 766,437 - +1.6 766,522 + +0.1 766,539 + -1.4 766,545 + -0.7 766,545 + -0.4 766,545 + -0.9 766,545 + -0.4 766,545 + -0.9 766,546 - +0.4 766,546 - -0.7 766,546 - +0.1 766,546 - +1.8 766,546 - +0.3 766,636 - +0.4 766,636 - -0.5 766,808 + -1.0 766,834 + SMc03068 0.11 -2.8 766,834 + SMc03068 0.11 -3.2 766,835 - SMc03068 0.11 -2.2 766,912 + SMc03068 0.15 -3.6 767,095 - SMc03068 0.25 -1.0 767,308 - SMc03068 0.37 -3.6 767,494 + SMc03068 0.47 -2.4 767,494 + SMc03068 0.47 -3.2 767,778 + SMc03068 0.63 -3.9 767,785 + SMc03068 0.63 -2.5 767,785 + SMc03068 0.63 -2.2 767,786 - SMc03068 0.63 -2.1 767,805 + SMc03068 0.64 -2.5 767,806 - SMc03068 0.64 -4.6 768,189 + SMc03068 0.85 -3.2 768,189 + SMc03068 0.85 -3.9 768,228 + SMc03068 0.88 -2.9 768,228 + SMc03068 0.88 -2.8 768,229 - SMc03068 0.88 -3.8 768,229 - SMc03068 0.88 -2.9 768,382 - -1.6 768,424 + -3.4 768,435 - -3.3 768,478 + -2.7 768,559 + -2.0 768,559 + -1.8 768,560 - -2.1 768,560 - +0.3 768,560 - -0.5 768,560 - -0.5 768,561 + -2.7 768,561 + -3.0 768,561 + -4.2 768,562 - +0.2 768,685 + SMc03069 0.20 -2.3 768,827 + SMc03069 0.40 -1.1 768,827 + SMc03069 0.40 -1.8 769,254 + -2.5 769,254 + -1.6 769,330 + -4.4 769,331 - -2.8
Or see this region's nucleotide sequence